We narrowed to 8,814 results for: CAG

1. px330_P53 exon 7 gRNA

   Plasmid

   #68351

   Purpose

   px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   P53 exon 7 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. px330_PTEN exon 5 gRNA

   Plasmid

   #68348

   Purpose

   px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   PTEN exon 5 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmCellFree_KA1_SARS-COV-2 _NSP1

   Plasmid

   #169376

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _NSP1
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Codon optimized for Saccharomyces cerevisiae

   Promoter

   T7- CMV/CAG

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pmCellFree_KA1_SARS-COV-2_N

   Plasmid

   #169401

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus N with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _N (N SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   July 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmCellFree_KA1_SARS-COV-2_ORF6

   Plasmid

   #169392

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Expression

   Mutation

   Promoter

   T7- CMV/CAG

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJOP-HTT-HR65Q

   Plasmid

   #92249

   Purpose

   HTT donor DNA with 65Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJOP-HTT-HR45Q

   Plasmid

   #92248

   Purpose

   HTT donor DNA with 45Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJOP-HTT-HR81Q

   Plasmid

   #92250

   Purpose

   HTT donor DNA with 81Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJOP-HTT-HR30Q

   Plasmid

   #92247

   Purpose

   HTT donor DNA with 30Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmCellFree_KA1_SARS-COV-2_ORF3a

    Plasmid

    #169390

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3a with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _ORF3a (ORF3a SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. px330_SMAD exon 9 gRNA

    Plasmid

    #68352

    Purpose

    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Insert

    SMAD exon 9 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmCellFree_KA1_SARS-COV-2_ORF7b

    Plasmid

    #169394

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7b with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _ORF7b (ORF7b SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. iF53 PB-Zim3-dCas9-mScarlet-Hygro

    Plasmid

    #204726

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. iF58 PB-Zim3-dCas9-mScarlet-Puro

    Plasmid

    #204728

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmCellFree_KA1_SARS-COV-2_ORF7a

    Plasmid

    #169393

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7a with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _ORF7a (ORF7a SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. iD20 LoxStopLox-Zim3-dCas9-mApple-hygro

    Plasmid

    #204720

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pmCellFree_KA1_Human-ACE2

    Plasmid

    #169406

    Purpose

    Cell-free/mammalian expression vector for Human ACE2 receptor with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    Human ACE2 receptor (ACE2 Human)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

    Plasmid

    #159630

    Purpose

    AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

    Depositor

    Andrew Allen, Ross Bathgate

    Article

    Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

    Insert

    Ast (AstA Fly)
    

    Use

    AAV

    Tags

    HA, IRES, mCherry, and neurophysin-1

    Expression

    Mammalian

    Mutation

    Promoter

    DCA (same as CAG promoter)

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTALYM3B15

    Plasmid

    #47878

    Purpose

    TALE-mClover against mouse major satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mClover
    

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTALYM3-Telo1

    Plasmid

    #47883

    Purpose

    TALE-mClover against telomere sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mClover
    

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTALYM4

    Plasmid

    #47875

    Purpose

    Destination plasmid with mRuby2 for Golden Gate reaction of TALE assembly

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Type

    Empty backbone

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTALYM16-Telo1

    Plasmid

    #47882

    Purpose

    TALE-mRuby2 against telomere sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. AAV:ITR-U6-sgRNA (Backbone) PCB-FlPO-WPRE-syntetisk pA-UTR

    Plasmid

    #68347

    Purpose

    AAV plasmid expressing the FlpO recombinase. with empty gRNA

    Depositor

    Jannik Elverløv-Jakobsen

    Insert

    FlPO
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTALYM9B15

    Plasmid

    #47877

    Purpose

    TALE-Ty1 against mouse major satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-Ty1
    

    Use

    TALEN

    Tags

    Ty1

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTALYM9

    Plasmid

    #47876

    Purpose

    Destination plasmid with 3xTy1 tags for Golden Gate reaction of TALE assembly

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Ty1tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pmCellFree_KA1_SARS-COV-2_RBD -N501Y

    Plasmid

    #169407

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 mutated RBD domain (N510Y, UK strain) of S protein with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _RBD (N501Y) (S SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    N501Y

    Promoter

    T7- CMV/CAG

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmCell-Free_KA1_SARS-COV-2_S-frag3

    Plasmid

    #169403

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus N-term fragment of S protein after the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    N-term frag. S protein after the furin cleavag (N SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmCellFree_KA1_SARS-COV-2_S-frag5

    Plasmid

    #169404

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus C-terminal ectodomain of S protein from the furin cleavag with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    C-term frag. S protein after the furin cleavag (S SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204722

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AICSDP-35:AAVS1-mEGFP

    Plasmid

    #91565

    Purpose

    Homology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP

    Depositor

    Allen Institute for Cell Science

    Insert

    AAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 7, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pTALYM4SpMi-05

    Plasmid

    #47880

    Purpose

    TALE-mRuby2 against Mus spretus/musculus minor satellite sequence

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTALYM3

    Plasmid

    #47874

    Purpose

    Destination plasmid with mClover for Golden Gate reaction of TALE assembly

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Type

    Empty backbone

    Use

    TALEN

    Tags

    mClover

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGag

    Plasmid

    #212630

    Purpose

    Expression vector for MLV Gag

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. CHR2-MBD

    Plasmid

    #21484

    Depositor

    Don Arnold

    Article

    Lewis et al Nat Neurosci. 2009 May . 12(5):568-76.

    Insert

    Channel Rhodopsin 2 EGFP Myosin Binding Domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTAL7b

    Plasmid

    #48706

    Purpose

    TALEN expression in mammalian cells

    Depositor

    Boris Greber

    Article

    Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FokI nuclease

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pTAL7a

    Plasmid

    #48705

    Purpose

    TALEN expression in mammalian cells

    Depositor

    Boris Greber

    Article

    Frank et al BMC Genomics. 2013 Nov 9;14(1):773.

    Type

    Empty backbone

    Use

    TALEN

    Tags

    FokI nuclease

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTALYM4SpMi-01

    Plasmid

    #47879

    Purpose

    TALE-mRuby2 against Mus spretus minor satellite sequence containing two SNPs

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Miyanari et al Nat Struct Mol Biol. 2013 Oct 6. doi: 10

    Insert

    TALE-mRuby2
    

    Use

    TALEN

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA

    Plasmid

    #139513

    Purpose

    B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Insert

    CAG rox stop rox td-sfGFP dre reporter
    

    Use

    Mouse Targeting; Dre/rox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGag-mCherry

    Plasmid

    #212631

    Purpose

    Expression vector for an MLV Gag-mCherry fusion protein

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    MLV Gag (UniProt ID: P03332) (gag MoMLV)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. iF67 PB-Zim3-XTEN-dCas9-mScarlet-Bsd-Neo-mAmetrine

    Plasmid

    #204732

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204729

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

    Plasmid

    #204727

    Purpose

    Frt-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

    Plasmid

    #204724

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. iF51 PB-Zim3-dCas9-mScarlet-Hygro-Neo-SnapTag

    Plasmid

    #204725

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204723

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAAV-FLEX-tTA

    Plasmid

    #149363

    Purpose

    AAV-mediated and Cre-dependent expression of tTA under the CAG promoter.

    Depositor

    Takeshi Imai

    Article

    Leiwe et al bioRxiv 2022.10.20.512984

    Insert

    tTA2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. Ornithine transcarbamylase (OTC)

    Plasmid

    #71877

    Purpose

    For mitochondrial unfolded protein response research

    Depositor

    Nicholas Hoogenraad

    Article

    Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

    Insert

    OTC (Otc Rat)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV2-GCaMP6f-P2A-PAmCherry

    Plasmid

    #133412

    Purpose

    Fluorescent reporter for calcium imaging and photoactivation.

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP6f-P2A-PAmCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSLQ11353

    Plasmid

    #183098

    Purpose

    pHR-CAG-sfGFP* ABE_3-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    GFP*
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV2-GCaMP5g-P2A-PAmCherry

    Plasmid

    #133411

    Purpose

    Fluorescent reporter for calcium imaging and photoactivation.

    Depositor

    Tim Holy

    Article

    Lee et al Science. 2019 Dec 13;366(6471):1384-1389

    Insert

    GCaMP5g-P2A-PAmCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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