We narrowed to 12,783 results for: NUC

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pMALp2x-SCO5461(43-204)

Plasmid

#42509

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Tags

maltose-binding protein

Expression

Bacterial

Mutation

deleted amino acids 1-42 (nucleotide 1-126)

Promoter

tac

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only
BII-gR-PtW-eSpCas9

Plasmid

#133357

Purpose

Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Tags

HA-tagged eSpCas9

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Trex2-bpA-EF1BFP

Plasmid

#111144

Purpose

Expression of mouse Trex2 and BFP reporter in mammalian cells

Depositor

Ralf Kuehn

Article

Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

Inserts

mouse Trex2 coding region (Trex2 Mouse)
BFP (Rnf112 )

Expression

Mammalian

Promoter

CAG and EF1

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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tdminiSOG-H2B-C-10

Plasmid

#57804

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Tags

tdminiSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

Plasmid

#127365

Purpose

Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Tags

GS linker, NLS, V5, and YFP

Expression

Plant

Mutation

R118G

Promoter

Arabidopsis UBQ10 promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-DN-CMV-GFP

Plasmid

#83252

Purpose

Adenoviral expression of STAT5A dominant negative with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Mutation

C-terminal deletion of 135 nucleotides (dominant …

Promoter

CMV

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Gcm2TEASY

Plasmid

#41031

Depositor

Nancy Manley

Article

Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

Insert

Gcm2 in situ probe (Gcm2 Mouse)

Use

In situ probe

Mutation

contains nucleotides 584-1510 of GenBank accessio…

Promoter

T7 sense; SP6 antisense

Available Since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pEY61

Plasmid

#191058

Purpose

acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(3.7k) (acr-2 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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sox10:PDGFRA wild-type

Plasmid

#98696

Purpose

I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

PDGFRA (PDGFRA Human)

Use

Zebrafish expression

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-DNL-CMV-GFP

Plasmid

#83253

Purpose

Adenoviral expression of STAT5A dominant negative with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5A (Stat5a Mouse)
eGFP

Use

Adenoviral

Mutation

C-terminal deletion of 351 nucleotides (dominant …

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHBS1412 GFP-TDP43 FYW-L

Plasmid

#118801

Purpose

To test the effect of sequence on nuclear body formation and splicing of full-length TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Lentiviral

Mutation

All FYW to L in CTD of full-length TDP43

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pp32ΔCT

Plasmid

#67241

Purpose

pp32(1-149) cloned into pProEX HTb

Depositor

Cynthia Wolberger

Article

Huyton et al Protein Sci. 2007 Jul;16(7):1308-15. Epu

Insert

pp32 (ANP32A Human)

Tags

His

Expression

Bacterial

Mutation

contains amino acid residues 1-149

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only
pLV.U6gT28.13.EFS-NS.H2B-RFP

Plasmid

#170388

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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p35S::IRE1a

Plasmid

#135232

Purpose

constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

Depositor

Armin Djamei

Article

Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

Insert

IRE1a (IRE1A Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

Plasmid

#118279

Purpose

Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

Depositor

Dietmar Schmitz

Article

Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

Inserts

mRuby2
ChR2(H134R)

Use

AAV and Cre/Lox

Tags

SV40 NLS and YFP

Promoter

EF1 alpha

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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sox10:PDGFRA mutant

Plasmid

#98697

Purpose

I-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafish

Depositor

Thomas Look

Article

Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

Insert

PDGFRA (PDGFRA Human)

Use

Zebrafish expression

Mutation

exon 8 and 9 deletion

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMyc2ElbLuc,

Plasmid

#53244

Purpose

reporter plasmid containing 2 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc2ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pSEM397 - mosTI unc-119 - NNN - operon - gfp - nls - tbb-2

Plasmid

#182354

Purpose

Nuclear GFP co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::gfp(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM399 - mosTI unc-119 - NNN - operon - mScarlet - nls - tbb-2

Plasmid

#182356

Purpose

Nuclear mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet(2xNLS)::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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(81) pcDNA3.1-BC2-Nup62-Flag-EPEA

Plasmid

#168094

Purpose

Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

Nup62-Ubc-Flag-EPEA

Tags

BC2-Tag, EPEA-tag, and Flag-tag

Expression

Mammalian

Mutation

P229A, S283T on Nup62 native sequence

Promoter

T7/CMV

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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