We narrowed to 5,443 results for: 129

1. mCherry-PREX1

   Plasmid

   #129619

   Purpose

   RhoGEF

   Depositor

   Dorus Gadella

   Article

   Reinhard et al Small GTPases. 2019 Aug 30:1-15. doi: 10

   Insert

   PREX1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH-EFIRES-P-Seipin-miniIAA7-3XFlag

   Plasmid

   #129722

   Purpose

   AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   Seipin (BSCL2 Human)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   miniIAA7-3XFlag

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   EF1a

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

   Plasmid

   #129706

   Purpose

   Express cre-dependent KASH-EGFP and Gmeb1 shRNA

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   KASH-EGFP
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

   Plasmid

   #129668

   Purpose

   A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   OsARF16-PB1 domain
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPomyces-SaCas9

   Plasmid

   #129553

   Purpose

   Contains codon-optimized SaCas9 and gRNA for streptomyces

   Depositor

   Fong Tian Wong, Mingzi M. Zhang

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   SaCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T-RhoA-T19N

   Plasmid

   #12960

   Depositor

   Gary Bokoch

   Insert

   RhoA DN (RHOA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   T19N Dominant negative

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-myc-Cdc42-wt

   Plasmid

   #12972

   Depositor

   Gary Bokoch

   Insert

   Cdc42 (CDC42 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #129418

   Purpose

   Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   SpCas9 and sgRAB18.N (RAB18 Human)
   

   Use

   CRISPR

   Tags

   T2A-mCherry-P2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR5pemIKpKan

   Plasmid

   #129271

   Purpose

   Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoter

   Depositor

   Lindsey Burbank

   Article

   Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-myc-Rac1-wt

    Plasmid

    #12985

    Depositor

    Gary Bokoch

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAH-CTX1-rhadCas9

    Plasmid

    #129391

    Purpose

    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Burkholderia cenocepacia codon-optimized dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Entire dCas9 codon optimized for the GC-rich B. c…

    Promoter

    PrhaBAD

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-FLEx-iSeroSnFR-EnhancedExport

    Plasmid

    #129180

    Purpose

    Fluorescent reporter for serotonin imaging (conditional + membrane localization tag with enhanced export sequence)

    Depositor

    Lin Tian

    Article

    Unger et al Cell. 2020 Dec 12. pii: S0092-8674(20)31

    Insert

    iSeroSnFR
    

    Use

    AAV

    Tags

    Ig-kappa leader, Myc, and PDGFR + enhanced ER exp…

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUG-P2A-tC3-W

    Plasmid

    #129475

    Purpose

    3rd-generation lentiviral vector for hUbC-driven co-expression of EGFP and truncated C3

    Depositor

    Robert Gross

    Insert

    truncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C

    Available since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP

    Plasmid

    #129111

    Purpose

    Homologous recombination vector for excision reporter 1 in the CLYBL locus.

    Depositor

    Bruce Conklin

    Insert

    LSL, eGFP, NLS-RFP
    

    Use

    Cre/Lox and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSL1394

    Plasmid

    #129522

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFLAG-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. K980

    Plasmid

    #129762

    Purpose

    Expresses Drosophila Kinesin-1 full-length for microtubule gliding assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Skylan-S-N1

    Plasmid

    #129737

    Purpose

    A green photo-switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)

    Depositor

    Pingyong Xu

    Article

    Zhang et al ACS Nano. 2015 Mar 24;9(3):2659-67. doi:

    Type

    Empty backbone

    Use

    Tags

    Skylan-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-myc-Rac1-T17N

    Plasmid

    #12984

    Depositor

    Gary Bokoch

    Insert

    Rac1 DN (RAC1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T-1-3xFLAG

    Plasmid

    #129570

    Purpose

    Empty backbone for FLAG tag

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ITPKA_3xmNeonGreen

    Plasmid

    #129606

    Purpose

    In vivo visualization of F-actin (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    F-tractin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pT2ADWpuro_2paRAF

    Plasmid

    #129653

    Purpose

    For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CMV-Tagger

    Plasmid

    #129396

    Purpose

    Expresses 2A-separated rpl22, UPRT, mKate2 and Ago2 driven by CMV promoter

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS Biol. 2019 Aug 8;17(8):e3000374. do

    Insert

    rpl22-HA-2A-UPRT-mKate2-FLAG-V5-Ago2
    

    Use

    Tags

    FLAG, HA, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBBR5pemIKpBAD

    Plasmid

    #129273

    Purpose

    Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoter

    Depositor

    Lindsey Burbank

    Article

    Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA6a-mNeonGreen-HIS3MX6

    Plasmid

    #129100

    Purpose

    For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen

    Depositor

    Wei-Lih Lee

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFP-hSV1_WT

    Plasmid

    #129483

    Purpose

    Mammalian expression of full-length human supervillin, isoform 1.

    Depositor

    Elizabeth Luna

    Article

    Smith et al Cytoskeleton (Hoboken). 2010 Jun;67(6):3

    Insert

    Supervillin (SVIL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3 AIF-mClover

    Plasmid

    #129575

    Purpose

    Expression of mClover fused AIF

    Depositor

    Stephen Tait

    Article

    Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

    Insert

    AIF (AIFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBADHISTEV_PfeE

    Plasmid

    #129120

    Purpose

    pBADHISTEV carrying pfeE for overexpression of PfeE in E. coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed

    Promoter

    PBAD

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCR2.1-KRAS-G12V

    Plasmid

    #129091

    Purpose

    KI KRAS G12V mutation into human KRAS locus

    Depositor

    Toshiro Sato

    Article

    Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

    Insert

    KRAS(G12V) (KRAS Human)
    

    Use

    Homologous recombination donor plasmid

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSCB2-sgRNA

    Plasmid

    #129463

    Purpose

    Template plasmid for guide sequence insertion via inverse PCR. Derived from pSCrhaB2-gRNA (see paper).

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    pgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p1195-pssAAV.U1a.hNme2Cas9

    Plasmid

    #129534

    Purpose

    AAV vector expressing Nme2Cas9

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    human codon-optimized Nme2Cas9
    

    Use

    AAV

    Tags

    2xNLS and NLS-3xHA-NLS

    Expression

    Mutation

    Promoter

    U1a

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge