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1,878 results

Showing: 251 - 300 of 1878 results

  4. pOD2048-csnDEG

    Plasmid
    #89368
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Insert
    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Promoter
    Posm-6
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. pJW2172

    Plasmid
    #163096
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163096-plasmid-map-sequence-id-320618

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  11. pCFJ1955

    Plasmid
    #84830
    Purpose
    Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized tdTomato
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84830-plasmid-map-sequence-id-163668

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  20. pSX2719

    Plasmid
    #200763
    Purpose
    Expresses mCherry fused MS2 coat protein and 24xGCN4 (Suntag) with the tissue-specific promoter semo-1 in C. elegans.
    Depositor
    Suhong Xu
    Article
    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755
    Insert
    MCP-24xGCN4
    Expression
    Worm
    Promoter
    semo-1
    Available Since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    200763-plasmid-map-sequence-id-402659

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  21. pSX2720

    Plasmid
    #200764
    Purpose
    Expresses Superfolder GFP-tag fused single change variable fragment antibody of GCN4 (Suntag) with the tissue-specific promoter col-19 in C. elegans.
    Depositor
    Suhong Xu
    Article
    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755
    Insert
    scFv-sfGFP
    Expression
    Worm
    Promoter
    col-19
    Available Since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    200764-plasmid-map-sequence-id-400888

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  25. pCC324

    Plasmid
    #110165
    Purpose
    miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    R61K change made in ERK-KTR CDS
    Promoter
    lin-31 promoter/enhancer
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110165-plasmid-map-sequence-id-235338

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  26. pCFJ352

    Plasmid
    #30539
    Depositor
    Erik Jorgensen
    Insert
    ttTi4348 region
    Expression
    Worm
    Available Since
    June 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    30539-plasmid-map-sequence-id-14101

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  28. pJW1583

    Plasmid
    #121054
    Purpose
    GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    GFP^SEC^AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, C. elegans codon optimized GFP, and auxin…
    Expression
    Worm
    Available Since
    June 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    121054-plasmid-map-sequence-id-235990

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  34. pJJR81

    Plasmid
    #75029
    Purpose
    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Mike Boxem
    Insert
    tagBFP2^SEC^3xFlag
    Use
    CRISPR
    Tags
    3xFLAG and C. elegans codon optimized tagBFP2
    Expression
    Worm
    Available Since
    June 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    75029-plasmid-map-sequence-id-141917

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  35. pOD2047-bwmControl

    Plasmid
    #100006
    Purpose
    MosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).
    Depositor
    Karen Oegema
    Article
    Wang et al Development. 2017 Jun 15. pii: dev.15009
    Inserts
    zif-1 (zif-1 Nematode)
    unc-119
    Use
    Targeting vector for mos1 transposon mediated sin…
    Available Since
    Sept. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    100006-plasmid-map-sequence-id-193783

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  39. pJJS008

    Plasmid
    #188331
    Purpose
    PCR template for repair templates to tag a gene with a wrmScarlet and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Article
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::wrmScarlet::mIAA7
    Expression
    Bacterial
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188331-plasmid-map-sequence-id-370747

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Showing: 251 - 300 of 1878 results