We narrowed to 336 results for: PREP

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pAD-LyseR (in lacZ deficient strain)

Plasmid

#99245

Purpose

For autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

Depositor

Jeff Hasty

Article

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Insert

phage lambda gene R (R phage lambda)

Expression

Bacterial

Promoter

beta-lactamase promoter

Available Since

Oct. 14, 2017

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mdnTcf4Ben (CT#1130)

Plasmid

#14020

Depositor

Cliff Tabin

Article

Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

Insert

Tcf4B (Tcf7l2 Mouse)

Use

Retroviral; Avian expression

Mutation

Dominant negative Tcf4B, engrailed fusion

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) mTcf4B (CT#1126)

Plasmid

#14019

Depositor

Cliff Tabin

Article

Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

Insert

Tcf4B (Tcf7l2 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pCEP4 Pig4

Plasmid

#16498

Depositor

Bert Vogelstein

Article

Polyak et al Nature. 1997 Sep 18. 389(6648):300-5.

Insert

Pig4 (SAA1 Human)

Tags

HA tag variant - YAYDVPDYA

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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LB1353 (Bcl-2 promoter, no IgH enh)

Plasmid

#15393

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 with full promoter and no IgH enhancers MHS…

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pet16b-Avi-ubiquitin_rbs_BirA

Plasmid

#134897

Purpose

Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Inserts

Ubiquitin (UBB Human)
BirA

Tags

10HIS-AviTag-PreScission(3C)protease

Expression

Bacterial

Promoter

RBS and T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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rAAV2/cMG.QRP

Plasmid

#184543

Purpose

AAV packaging plasmid, expressing Rep2 and AAV-cMG.QRP capsid

Depositor

Minmin Luo

Article

Lin et al Nat Methods. 2022 Jul 25. pii: 10.1038/s

Insert

AAV-cMG.QRP capsid

Use

AAV; Helper vector for packaging

Mutation

changes on AAV9 capsid: K449R; insertion of QRPPR…

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Flag_HsIRE1a_deltaP29_D408_pBabePuro

Plasmid

#58422

Purpose

retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408

Depositor

David Ron

Article

Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

Insert

IRE1a (ERN1 Human)

Use

Retroviral

Tags

FLAG and preprotrypsin signal sequence

Expression

Mammalian

Mutation

Deleted amino acids P29 to D408

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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LB1366 (Bcl-2 promoter with MHS1234)

Plasmid

#15388

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with IgH full enhancer MHS1234

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pJBL7010

Plasmid

#136942

Purpose

Constitutive sfGFP expression (high), bacterial promoter

Depositor

Julius Lucks

Article

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPE4max-Blasticidin

Plasmid

#194905

Purpose

Expressing prime editing PE4max proteins, to work in conjunction with an epegRNA encoding plasmid or Circular Vector as described in the cited article.

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2022 Dec 21;5(1):1393. doi:

Insert

BSD

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMyc4ElbLuc.

Plasmid

#53246

Purpose

reporter plasmid containing 4 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc4ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pVP2A-PIF6

Plasmid

#73369

Purpose

Expresses Adeno-associated virus VP2 with PIF6 insert.

Depositor

Junghae Suh

Article

Gomez et al ACS Nano. 2015 Nov 30.

Insert

PIF6 (PIL2 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pFastbac-Dual-StrepII-BARD1

Plasmid

#137166

Purpose

Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

BARD1 (BARD1 Human)

Tags

StrepII

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

p10

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pFastbac1-Flag-FANCD2

Plasmid

#134904

Purpose

codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

FANCD2 (FANCD2 Human)

Tags

Flag

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

polyhedron

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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WT (REP10)

Plasmid

#21368

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Expression

Mammalian

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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HOXA9-MSCV-CD24

Plasmid

#20975

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

Inserts

HOXA9 (Hoxa9 Mouse)
CD24 (CD24 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Full length cDNA with ~1.1kb 3'UTR with 2 pu…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only

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RISAP act beta-catenin (CT#1147)

Plasmid

#14041

Depositor

Cliff Tabin

Article

Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

Insert

beta catenin (ctnnb1.L Frog)

Use

Retroviral; Avian expression

Mutation

Activated form

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pMyc3ElbLuc

Plasmid

#53245

Purpose

reporter plasmid containing 3 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc3ElbLuc (MYC Human)

Mutation

To prepare plasmids with one, two, three, and fou…

Available Since

July 29, 2014

Availability

Academic Institutions and Nonprofits only