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lentiCRISPR - EGFP sgRNA 1

Plasmid

#51760

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Publication

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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BirA in pET28a (w400-2)

Plasmid

#26624

Depositor

Eric Campeau

Insert

BirA

Tags

His

Expression

Bacterial

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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2XMyc-LRRK2-WT

Plasmid

#25361

Depositor

Mark Cookson

Publication

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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mEGFP-HRas G12V

Plasmid

#18666

Depositor

Karel Svoboda

Publication

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

G12V mutation in HRas

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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multi-shRNA vector

Plasmid

#12391

Depositor

Zuoshang Xu

Publication

Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Insert

mSOD2 shRNA (Sod2 Mouse)

Use

RNAi

Expression

Mammalian

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK792

Plasmid

#8830

Depositor

David Waugh

Publication

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219P mutant

Tags

His, MBP (cleaved), and polyarginine

Expression

Bacterial

Mutation

S219P mutation

Available since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBABE hBRM

Plasmid

#1961

Depositor

Robert Kingston

Publication

Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

Insert

BRM (SMARCA2 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCEP4-myc-ACE2

Plasmid

#141185

Purpose

Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag

Depositor

Erik Procko

Publication

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

35 Articles

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pCS2+HyPer7-NES

Plasmid

#136467

Purpose

Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Publication

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Nuclear export signal from the HIV Rev protein

Expression

Mammalian

Promoter

CMV, SP6

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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C1(1-29)-TurboID-V5_pLX304

Plasmid

#107175

Purpose

expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Publication

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Use

Lentiviral

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR-mCh-Cry2WT

Plasmid

#101221

Purpose

Blue-light dependent optogenetic protein Cry2

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

Cry2PHR

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

SFFV

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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RG6

Plasmid

#80167

Purpose

fluorescent protein splicing reporter

Depositor

Thomas Cooper

Publication

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Expression

Mammalian

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pHR_Gal4UAS_IRES_mC_pGK_tBFP

Plasmid

#79123

Purpose

Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors

Depositor

Wendell Lim

Publication

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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APEX2-GBP

Plasmid

#67651

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

Depositor

Rob Parton

Publication

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAVU6+27-F30-2xdBroccoli

Plasmid

#66842

Purpose

Expresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

Depositor

Samie Jaffrey

Publication

Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

Insert

Two dimeric Broccoli aptamers in the F30 scaffold

Expression

Mammalian

Promoter

U6

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCRCT

Plasmid

#60621

Purpose

Plasmid encoding iCas9, tracrRNA and crRNAs

Depositor

Huimin Zhao

Publication

Bao et al ACS Synth Biol. 2014 Sep 19.

Inserts

iCas9
tracrRNA

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Yeast

Mutation

changed Aspartate 147 to Tyrosine, Proline 411 to…

Promoter

RPR1p and TEF1p

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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PH-Btk-GFP

Plasmid

#51463

Purpose

Is a biosensor for PIP3

Depositor

Tamas Balla

Publication

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

BTK (aa 1–177) (BTK Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCAG-Brainbow3.0

Plasmid

#45176

Depositor

Joshua Sanes

Publication

Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

Insert

pCAG-Brainbow3.0

Expression

Mammalian

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

Plasmid

#44028

Depositor

Celeste Simon

Publication

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Insert

mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P402A, P577A, N813A

Promoter

CMV

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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wtTDP43tdTOMATOHA

Plasmid

#28205

Depositor

Zuoshang Xu

Publication

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

HA tag and tdtomato

Expression

Mammalian

Available since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pBPp65ADZpUw

Plasmid

#26234

Depositor

Gerald Rubin

Publication

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

p65AD-Zip (RELA Human)

Expression

Insect

Available since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

24 Articles

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pMSCV-OPA1

Plasmid

#26047

Depositor

David Chan

Publication

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

OPA1 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMA2640

Plasmid

#25434

Purpose

Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

Depositor

Mikhail Alexeyev

Publication

Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

Insert

reverse tetracycline-regulated transactivator Advanced

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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Myc-TEAD4

Plasmid

#24638

Depositor

Kunliang Guan

Publication

Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

Insert

TEAD-4 (TEAD4 Human)

Tags

Myc

Expression

Mammalian

Available since

May 13, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMXs-DsRed Express

Plasmid

#22724

Depositor

Shinya Yamanaka

Publication

Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

Insert

a variant of Discosoma sp. red fluorescent protein

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pENTR4-GFP-C1 (w392-1)

Plasmid

#17396

Purpose

Gateway entry vector for N-term eGFP fusion, frame C1

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Gateway Cloning

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pRetrosuper Myc shRNA

Plasmid

#15662

Depositor

Martin Eilers

Publication

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Myc shRNA (MYC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pST1374

Plasmid

#13426

Depositor

Keith Joung

Publication

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Type

Empty backbone

Tags

Flag

Expression

Mammalian

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pMXs-c-Myc-T58A

Plasmid

#13372

Purpose

Retroviral expression of mouse c-Myc T58A mutant

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (T58A mutant) (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Tyrosine 58 to Alanine

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEG BacMam

Plasmid

#160451

Purpose

vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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