We narrowed to 8,624 results for: FIE
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Plasmid#62948PurposeCreating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1DepositorInsertT2A-LexA::QFAD-Hsp70
Available SinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6sgbbBsmbI-puro-2A-Fluc
Plasmid#100277PurposeLentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imagingDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
hDAGLb-V5
Plasmid#87677PurposeExpresses human DAGLb protein with intracellular V5 tag located on the C-terminalDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3
Plasmid#62957PurposeTriplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4 in three phases
Available SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP8 (full length))
Plasmid#16361DepositorInserthuman SENP8 full length (SENP8 Human)
TagsHisExpressionBacterialMutationhuman SENP8 full length, see Depositor CommentsAvailable SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1209
Plasmid#44491DepositorInsertMinimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, NeoR]
UseGateway expression cloneAvailable SinceApril 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV Cep164 (Nigg CW325)
Plasmid#41148DepositorAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
penaav-tnt-3xha-p2a-gfp
Plasmid#175090PurposeFor making AAV9DepositorTypeEmpty backboneUseAAVAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-K122-TEAD-p-H2B-mCherry
Plasmid#139648PurposeReporter assayDepositorInsertIRES-GFP
UseLentiviralAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-Decoy
Plasmid#80324PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondiiDepositorInsertDecoy sgRNA
UseCRISPRPromoterU6Available SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgKmt2d-sg1
Plasmid#192166PurposeLenti-sgKmt2d-sg1DepositorTypeEmpty backboneUseLentiviralMutationNAAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
mcherry-Myo 9b R1695M
Plasmid#134958PurposeExpression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.DepositorInsertMyo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
TagsmcherryExpressionMammalianPromoterCMV IEAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-ING2
Plasmid#109502PurposeMuscle specific zebrafish expression of mKate2 fused to 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsertactc1b-mKate2-ING2
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tmem214 pmRFP-N2 (1348)
Plasmid#62047Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLC-241
Plasmid#73194PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorAvailable SinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP5 (catalytic domain)
Plasmid#16358DepositorInserthuman SENP5 catalytic domain (SENP5 Human)
TagsHisExpressionBacterialMutationAmino Acid 567 to 755 catalytic domain of human S…Available SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pOPO717
Plasmid#195302PurposepBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.DepositorInsertMcCAST Cascade and lacZ spacer 5 in native array
ExpressionBacterialPromoterArabinose inducible arapBADAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Syn-FLEX-ExSYTE
Plasmid#216263PurposeCre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoterDepositorInsertExSYTE P2A EGFP - Rat DTE
UseAAVAvailable SinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tmem214 pEGFP-N2 (1072)
Plasmid#62026Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry
Plasmid#139649PurposeReporter assayDepositorInsertH2B-mCherry
UseLentiviralTagsFLAG, His6Available SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pME18ST-d5-HT7
Plasmid#53111PurposeExpression of Drosophila 5-HT7DepositorInsert5HT7 (5-HT7 Fly)
Available SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-YPet-WPRE
Plasmid#193342PurposeAAV-mediated expression of YPet under the TRE promoter in mammalian cellsDepositorInsertYPet
UseAAVExpressionMammalianPromoterTREAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro
Plasmid#170467PurposeThe plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistanceDepositorArticleInsertNucleocapsid
UseLentiviralTagsmNeonGreenExpressionMammalianPromoterEF1Available SinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
FOR human NANOS3 TALEN
Plasmid#59722PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertHD HD NG NH HD NG HD NG HD HD HD NG HD HD NI NG
UseTALENExpressionMammalianPromoterCAGGSAvailable SinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
REV human NANOS3 TALEN
Plasmid#59723PurposeTALEN expressing vector used to allow Knock-in of NANOS3-P2A-mCherry knockin donor plasmid (addgene #59721)DepositorInsertNG NH HD HD HD NI HD HD NG NH NG NI NH NH HD NI
UseTALENExpressionMammalianPromoterCAGGSAvailable SinceDec. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_703128
Plasmid#224394PurposeFor expression of the type I CBASS system encoded by Escherichia coli 703128DepositorInsertEcCdnD_4TM
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_BWH59
Plasmid#224393PurposeFor expression of the type I CBASS system encoded by Escherichia coli BWH59DepositorInsert2TM_EcCdnD
ExpressionBacterialAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-mRuby3-WPRE
Plasmid#193343PurposeAAV-mediated expression of mRuby3 under the TRE promoter in mammalian cellsDepositorInsertmRuby3
UseAAVExpressionMammalianPromoterTREAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-TetR-KRf-TetR
Plasmid#216270PurposeConstruct 3: TetR - Lipid binding domain KRφ - TetRDepositorInsertTetR - Lipid binding motif (KRf) - TetR
TagsEGFPExpressionMammalianAvailable SinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME18ST-d5-HT1B
Plasmid#53108PurposeExpression of Drosophila 5-HT1BDepositorInsert5HT1B (5-HT1B Fly)
Available SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME18ST-d5-HT2A
Plasmid#53109PurposeExpression of Drosophila 5-HT2ADepositorInsert5HT2A (5-HT2 Fly)
Available SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pME18ST-d5-HT1A
Plasmid#53107PurposeExpression of Drosophila 5-HT1ADepositorInsert5HT1A (5-HT1A Fly)
Available SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_TLOC1 (399aa)
Plasmid#45288DepositorAvailable SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMP71-tCD19-LLO-SIINFEKL
Plasmid#174598Purposeexpresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the LLO190 and SIINFEKL epitopesDepositorInsertmembrane bound CD19, LLO antigen, SIINFEKL (ovalbumin) antigen
ExpressionMammalianAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-ING2
Plasmid#109554PurposeMultisite gateway vector for 3' tagging with 3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised. Binds PtdIns5PDepositorInsert3xPHD domain from Mouse inhibitor of growth family member 2 (ING2), codon optimised
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateMutationThe 3’-end of the chloramphenicol resistance gene…Available SinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Bin1b
Plasmid#109509PurposeMuscle specific zebrafish expression of mKate2 fused to Bin1bDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
FGF10-luciferase
Plasmid#24994DepositorAvailable SinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN-Tpl2/Cot
Plasmid#27558DepositorAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only