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Showing: 2361 - 2400 of 17141 results

  3. XLone-Puro SPI1 P2A mCherry

    Plasmid
    #140029
    Purpose
    Tunable and temporal expression control of SPI1 and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SPI1 (SPI1 Human)
    Use
    Synthetic Biology
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    140029-plasmid-map-sequence-id-275844

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  15. 1272 pBabe puroL Myr HA Akt3

    Plasmid
    #9019
    Depositor
    William Sellers
    Insert
    Akt3 (AKT3 Human)
    Use
    Retroviral
    Tags
    HA and Myr
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 2, 2005
    Availability
    Academic Institutions and Nonprofits only

  18. pdCas9-DNMT3A-PuroR (ANV)_v2

    Plasmid
    #74408
    Purpose
    Expression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A S. pyogenes, Human, Synthetic)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    CBh
    Available since
    April 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74408-plasmid-map-sequence-id-145566

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  21. pLenti-CMV-tdTomato-miRE_shRen-Puro

    Plasmid
    #135687
    Purpose
    Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135687-plasmid-map-sequence-id-327656

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  26. pLenti CMV Puro DHFR.dn-cHSF1

    Plasmid
    #134729
    Purpose
    Lentiviral expression vector for constitutively active dominant-negative HSF1 fused to DHFR destabilized domain
    Depositor
    Matthew D. Shoulders
    Article
    Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.
    Insert
    DHFR.dn-cHSF1 (HSF1 Human)
    Use
    Lentiviral
    Tags
    DHFR
    Expression
    Mammalian
    Mutation
    Deletion of amino acids 186-202, 379−529
    Promoter
    CMV
    Available since
    Aug. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    134729-plasmid-map-sequence-id-271703

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  32. pLKO-Tet-puro-hRAF1-shRNA-1

    Plasmid
    #185371
    Purpose
    For tetracycline-inducible mammalian expression of shRNA: CATGAGTATTTAGAGGAAGTA that targets human RAF1
    Depositor
    Ayaz Najafov
    Insert
    RAF1 (RAF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    185371-plasmid-map-sequence-id-363455

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  36. PEA1-Puro-HEK3 CTT ins

    Plasmid
    #171996
    Purpose
    Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171996-plasmid-map-sequence-id-338997

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Showing: 2361 - 2400 of 17141 results