We narrowed to 22,079 results for: his

1. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

   Plasmid

   #205256

   Purpose

   S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS

   Plasmid

   #187358

   Purpose

   Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag

   Depositor

   Simon Davis

   Article

   Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

   Insert

   HPC-4 antibody heavy chain, and 6xHIS tag
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

   Plasmid

   #183069

   Purpose

   Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c_4
   

   Use

   CRISPR

   Tags

   His10 and MBP

   Expression

   Bacterial

   Mutation

   Wild-type

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTWIN1-His6-Ssp-Httex1-23Q

   Plasmid

   #84349

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Use

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28a-mScarlet-TEV-Strep-His

   Plasmid

   #210823

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mScarlet fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mScarlet
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPPI4-Strep-SNAP-CTLA-4-His6

   Plasmid

   #223583

   Purpose

   Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tag from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   Tags

   His6 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-Fgß-ddFLN4-HIS-ybbr

   Plasmid

   #101239

   Purpose

   wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   Fgß peptide, ddFLN4 fingerprint (FGB Human, D. discoideum)
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6

   Plasmid

   #180313

   Purpose

   bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV and sfCherry2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-Duet1_6xHis-TEV-GABARAP-Gly

   Plasmid

   #190930

   Purpose

   Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   GABARAP (GABARAP Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Ends with Glycine 116

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-CohIII-CBM-HIS-LPETGG

    Plasmid

    #91697

    Purpose

    CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    CohIII-CBM-HIS-LPETGG
    

    Use

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1/CIP2A(1-905) L533E V5 His

    Plasmid

    #119288

    Purpose

    CIP2A L533E V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    L533E

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. His6tev CBP BCHZ (1079-1751)

    Plasmid

    #99341

    Purpose

    Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
    

    Use

    Tags

    His6tev

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMJ240: pRS426-His-MBP-MmeFz2_Native

    Plasmid

    #205255

    Purpose

    S. cerevisiae expression vector for MmeFz2 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    MmeFz2
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

    Plasmid

    #78509

    Purpose

    Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD45R0Ex (PTPRC Human)
    

    Use

    Tags

    His10 and SNAPf

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMG81-MBP-t-M.MpeI-N374K-His

    Plasmid

    #197985

    Purpose

    Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

    Depositor

    Rahul Kohli

    Article

    Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

    Insert

    MBP-t-M.MpeI-N374K-His
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    M.MpeI-N374K

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid

    #111573

    Purpose

    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    lysine to glutamine mutation at the following ami…

    Promoter

    polyhedrin promoter

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSUMO His6 SUMO PYL1 (36-211)

    Plasmid

    #177843

    Purpose

    Bacterial Expression of PYL1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    PYR1-like 1 (PYL1 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSUMO His6 SUMO AMPKa (11-281)

    Plasmid

    #177851

    Purpose

    Bacterial Expression of AMPKa1

    Depositor

    Karsten Melcher

    Article

    Li et al Cell Res. 2015 Jan;25(1):50-66. doi: 10.

    Insert

    AMPK a1 (PRKAA1 Human)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-His10-SHP1_PTPase (243-541aa)

    Plasmid

    #180812

    Purpose

    Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SHP1_PTPase (243-541aa) (PTPN6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 LIC 6A HIS-TEV-DPP9S

    Plasmid

    #166791

    Purpose

    Mammalian cell expression vector for His-tagged DPP9S

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. HOXB8 (murine) HIS-tag pET

    Plasmid

    #8540

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB8 (Hoxb8 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    starts at amino acid #2 (Ser)

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3.1V5-His Snk/Plk2 T239D

    Plasmid

    #22220

    Depositor

    Wafik El-Deiry

    Article

    Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

    Insert

    Snk/Plk2 (PLK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    single amino acid substitution mutant, threonine …

    Promoter

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pETNKI-his-SUMO-RNF168ring (1-189)

    Plasmid

    #63570

    Purpose

    Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    6xHis and SUMO3

    Expression

    Bacterial

    Mutation

    Ring domain only (residues 1-189)

    Promoter

    T7

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.1/Myc-His(-)-HSulf-1 deltaCC

    Plasmid

    #13005

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 delta CC (SULF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 87 to Alanine

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

25. pET-His6-Sumo-MBP-CfaN

    Plasmid

    #126016

    Purpose

    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

    Depositor

    Thomas Muir

    Insert

    His5-Sumo-MBP-CfaN
    

    Use

    Tags

    5xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMJ235: pRS426-His-MBP-GtFz1_Native

    Plasmid

    #205254

    Purpose

    S. cerevisiae expression vector for GtFz1 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    GtFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-28a-His6-SUMO-VAMP3

    Plasmid

    #135552

    Purpose

    Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    VAMP3
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNATM3.1/V5-His TOPO-PKCi-K274W

    Plasmid

    #55690

    Purpose

    expresses dominant negative human PKCi tagged with V5

    Depositor

    Pedro Salas

    Article

    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

    Insert

    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    A120E and K274W

    Promoter

    CMV

    Available since

    Aug. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pET29b-eGFP-dspB(E184Q)-6xHis

    Plasmid

    #175783

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and eGFP

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET29b-mVenus-dspB(E184Q)-6xHis

    Plasmid

    #176576

    Purpose

    Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

    Depositor

    Mark Nitz

    Article

    Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

    Insert

    Dispersin B
    

    Use

    Tags

    Hexahistidine tag and mVenus

    Expression

    Bacterial

    Mutation

    E184Q

    Promoter

    T7

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Ana GvpC-SpyTag-His in pET28a

    Plasmid

    #85737

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (fused to SpyTag and His-tagged)
    

    Use

    Tags

    His-tag and SpyTag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCI_E01_mini-mAgrin-Myc-His-WPRE__LG1-3

    Plasmid

    #198141

    Purpose

    Express mini-Agrin in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    mini-Agrin (Agrn Mouse)
    

    Use

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCI_E01_mini-mAgrin-Myc-His-WPRE__LG3

    Plasmid

    #198140

    Purpose

    Express mini-Agrin in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    mini-Agrin (Agrn Mouse)
    

    Use

    Tags

    TEV-Myc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, beta-globin/IgG chimeric intron

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-hsyn_NES-His-rsCaMPARI-mRuby3

    Plasmid

    #120805

    Purpose

    AAV plasmid for expressing rsCaMPARI in neurons, nucleus excluded

    Depositor

    Eric Schreiter

    Article

    Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

    Insert

    rsCaMPARI
    

    Use

    AAV

    Tags

    NES-His and mRuby3

    Expression

    Mutation

    Promoter

    hsyn (synapsin-1)

    Available since

    Oct. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLenti-Blast PhLP1-myc-6xHis

    Plasmid

    #218178

    Purpose

    Used to make a lenti virus for stable expression of phosducin-like protein 1 in mammalian cells

    Depositor

    Barry Willardson

    Article

    Wang et al Mol Cell. 2023 Nov 2;83(21):3852-3868.e6

    Insert

    Phosducin like protein 1 (PDCL Human)
    

    Use

    Lentiviral

    Tags

    6x His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid

    #58711

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    Xyl-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Threonin 120 to Cysteine in Xylanase Doma…

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUb-HisUb-Ubc9-ChE1 v4.5

    Plasmid

    #185756

    Purpose

    Bacterial expression of His-Ub, Ubc9 and ChE1 v4.5

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Inserts

    His-Ub
    Ubc9
    ChE1 v4.5
    

    Use

    Tags

    His6

    Expression

    Mutation

    K14R

    Promoter

    T7

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. YIp128-CUP1-NUbo-GFP-His6

    Plasmid

    #212790

    Purpose

    Copper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeast

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    NUbo-GFP-His6
    

    Use

    Integrative vector

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNATM3.1/V5-His TOPO-PKCi-A120E

    Plasmid

    #55687

    Purpose

    expresses constitutively active human PKCi tagged with V5

    Depositor

    Pedro Salas

    Article

    Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

    Insert

    Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    A120E

    Promoter

    CMV

    Available since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

40. HOXA7 (murine) His-tag pET

    Plasmid

    #8491

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA7 (Hoxa7 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG; makes protein by …

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

41. His-ZZ-TEV-SNAPf-BICD2N(1-400)

    Plasmid

    #111857

    Purpose

    N-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICD2N(1-400)
    

    Use

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    Optimised for Sf9 expression

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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42. p6268 pDEST-59-His-Trx-HPV16E6

    Plasmid

    #24126

    Depositor

    Peter Howley

    Insert

    HPV16 E6 (E6, E6 HPV)
    

    Use

    Tags

    His and Trx

    Expression

    Bacterial and Mammalian

    Mutation

    none

    Promoter

    Available since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

43. pF712-pET28a-Hs-delta44-GCDH-NHis wt

    Plasmid

    #85113

    Purpose

    expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta44) GCDH (GCDH Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    silent SNP: G1041T

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET28a-ybbR-HIS-sfGFP-DocI

    Plasmid

    #58708

    Purpose

    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    sfGFP-DocI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pcDNA3.1 LIC 6A HIS-TEV-DPP8

    Plasmid

    #166793

    Purpose

    Mammalian cell expression vector for Flag-tagged DPP8

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP8 (DPP8 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

    Plasmid

    #167268

    Purpose

    Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HOB
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

    Plasmid

    #146392

    Purpose

    Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmeIF4G-trunc (eIF4G1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    322-1666 truncated version of elF4G sequence with…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. YIp128-P30-His-pol30(K164R)

    Plasmid

    #99544

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; Leu2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K164R

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pET29-hMIGA2(170-593)-6xhis

    Plasmid

    #192834

    Purpose

    Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pET-His6-Sumo-IntNtr-eSrt2A

    Plasmid

    #126015

    Purpose

    Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)

    Depositor

    Thomas Muir

    Insert

    His6-Sumo-IntNtr-eSrt2A
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge