We narrowed to 4,185 results for: Gca

1. ELAVL1 E6.3 gRNA

   Plasmid

   #90679

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ELAVL1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   ELAVL1 (Guide Designation E6.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRIP13 H4.2 gRNA

   Plasmid

   #90921

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIP13

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   TRIP13 (Guide Designation H4.2)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. CDC20 D4.1 gRNA

   Plasmid

   #90585

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDC20

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CDC20 (Guide Designation D4.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. BUB1 A6.1 gRNA

   Plasmid

   #90553

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BUB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BUB1 (Guide Designation A6.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. DSN1 C12.1 gRNA

   Plasmid

   #90657

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human DSN1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   DSN1 (Guide Designation C12.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSicoR-ATF7IPi1

   Plasmid

   #59614

   Purpose

   Expression of shRNA against human ATF7IP

   Depositor

   Miguel Ramalho-Santos

   Article

   Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

   Insert

   ATF7IP (ATF7IP Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

7. CMV-sgE1TSS-short-3'box

   Plasmid

   #92165

   Purpose

   sgRNA expression vector for Evx1as RNA tethering assay

   Depositor

   Xiaohua Shen

   Article

   Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

   Insert

   Evx1as short isoform
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-memRFP-3xPax7gRNA

   Plasmid

   #224569

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   CRISPR

   Tags

   Membrane localization signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-MCS-shCHD5 #2

   Plasmid

   #68877

   Purpose

   CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

    Plasmid

    #213973

    Purpose

    AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

    Depositor

    Zhonghua Lu

    Article

    Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

    Insert

    Shank3 sgRNA
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid

    #181745

    Purpose

    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    human/zebrafish codon optimized SpCas9
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. shRNA_circHUWE1_2

    Plasmid

    #215235

    Purpose

    Supression of shcircHUWE1(19,20)_2 expression

    Depositor

    Sefi Rosenbluh

    Article

    Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

    Insert

    circHUWE1 shRNA 2 (HUWE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9nucl-T2A-EGFP - BAXgRNA1- BAXgRNA2

    Plasmid

    #167296

    Purpose

    Plasmid encoding for 2 gRNAs targeting the human BAX gene and a CMV driven nuclease Cas9 followed by self-cleaving EGFP

    Depositor

    Agnel Sfeir

    Article

    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

    Insert

    BAX (BAX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MYH11-gRNA1

    Plasmid

    #132587

    Purpose

    gRNA used for knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele (MYH11-NanoLuc-2A-tdTomato vector) )

    Depositor

    James Thomson

    Article

    Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

    Insert

    MYH11-gRNA1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIV515

    Plasmid

    #177703

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmIL-6 mut AP-1+C/EBP

    Plasmid

    #61292

    Purpose

    drives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sites

    Depositor

    Gail Bishop

    Article

    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

    Insert

    IL-6 promoter (Il6 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Mutated both AP-1 binding site from TGAGTCT to TG…

    Promoter

    IL-6 promoter with mutant AP-1 and C/EBP binding …

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)

    Plasmid

    #195741

    Purpose

    Base editing for making a pre-stop codon in mmoX using phenol inducible system

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Po (phoenol-inducible promoter)

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDF0430 huDisCas7-11-S1006-GGGS-D1221-U6-pro-Gluc

    Plasmid

    #186987

    Purpose

    AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of Gluc crRNA guide

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    cas7-11-s1006-gggs-d122, Gluc crRNA guide
    

    Use

    AAV

    Tags

    Expression

    Mutation

    s1006-gggs-d1221 deletion

    Promoter

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAPM-miR30-MAVS-ts1

    Plasmid

    #174274

    Purpose

    MAVS knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    MAVS shRNA (MAVS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. L2_lacZgRNA-Cas9-CsA

    Plasmid

    #136138

    Purpose

    Plasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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