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We narrowed to 17,737 results for: EMB

Showing: 3151 - 3200 of 17737 results
  1. pJET-62-SFFV-T2A-PuroR-polyA-R11b

    Plasmid
    #179893
    Purpose
    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)
    Depositor
    Insert
    RAB11B, member RAS oncogene family (RAB11B Human)
    Use
    CRISPR; Donor for homologous based knock in
    Expression
    Bacterial and Mammalian
    Promoter
    SFFV
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pJET-64-SFFV-T2A-BlaR-polyA-R11b

    Plasmid
    #179895
    Purpose
    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)
    Depositor
    Insert
    RAB11B, member RAS oncogene family (RAB11B Human)
    Use
    CRISPR; Donor for homologous based knock in
    Expression
    Bacterial and Mammalian
    Promoter
    SFFV
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pIRESneo2 mycBioID-Tollip M240A,F241A

    Plasmid
    #178055
    Purpose
    myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
    Depositor
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Tags
    mycBioID
    Expression
    Mammalian
    Mutation
    Changed Methionine 240 to Alanine and changed Phe…
    Promoter
    CMV
    Available Since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
  4. FB026

    Plasmid
    #119711
    Purpose
    Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Insert
    Promoter gpdA:YFP coding sequence:Terminator trpC
    Use
    Synthetic Biology
    Available Since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pXeDNA3.1-HsTRPV1

    Plasmid
    #244454
    Purpose
    pXeDNA3.1-HsTRPV1 encodes human TRPV1 in CMV/Xenopus β‑globin UTR scaffold for robust expression in mammalian cells and Xenopus oocytes; BGH polyA, AmpR, NeoR, lacZα for blue‑white screening.
    Depositor
    Insert
    Transient receptor potential cation channel subfamily V member 1 (TRPV1 Human)
    Use
    Xenopus oocyte expression
    Expression
    Mammalian
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid
    #178057
    Purpose
    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.
    Depositor
    Insert
    XRI-FLAG
    Use
    AAV
    Tags
    FLAG-MBP
    Expression
    Mammalian
    Promoter
    Human ubiquitin C (UBC) promoter
    Available Since
    Oct. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pKK-TEV-ProteinA

    Plasmid
    #105788
    Purpose
    Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-ProteinA
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pKK-MBP-TEV

    Plasmid
    #105771
    Purpose
    Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    MBP-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pKK-TEV-EGFP

    Plasmid
    #105793
    Purpose
    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 8885998, 8805248). EGFP is not truly monomeric (PMID: 11988576, 22869113)
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-EGFP
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pKK-mCherry-TEV

    Plasmid
    #105779
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 15558047). mCherry descends from the first truly monomeric red FP mRFP1.
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mCherry-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pKK-TEV-mRuby3

    Plasmid
    #105797
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mRuby3
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pKK-mRuby3-TEV

    Plasmid
    #105780
    Purpose
    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 26879144). mRuby3 is brighter than mCherry and has shifted ex and em spectra.
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mRuby3-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pcDNA3.1-nAChr-Alpha6-mCherry

    Plasmid
    #50480
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    mCherry fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  14. pKK-TEV-MBP

    Plasmid
    #105787
    Purpose
    Expression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-MBP
    Expression
    Mammalian
    Available Since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 3151 - 3200 of 17737 results