We narrowed to 29,446 results for: REP

1. pACEBac1_Strep-Psc-BICD2(711-800)_L786A

   Plasmid

   #228838

   Purpose

   Insect cell expression of the mutant C-terminus of BICD2

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 711-800, with L786A

   Promoter

   Available since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACEBac1_Strep-Psc-BICD2(711-800)_L742A

   Plasmid

   #228839

   Purpose

   Insect cell expression of the mutant C-terminus of BICD2

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 711-800, with L742A

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPICZ A-SLC-His+Strep-Opa1 (SB254)

   Plasmid

   #227603

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   Opa1
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 253-960 only

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

   Plasmid

   #227608

   Purpose

   Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   aa 2-83 deleted

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

   Plasmid

   #227609

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   contains aa 2-544, 651-757 only [TM deleted]

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPICZ A-SLC-His+MFN2-Strep (SB255)

   Plasmid

   #227604

   Purpose

   Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Inserts

   SLC25A46 (SLC25A46 Human)
   MFN2 (MFN2 Human)
   

   Use

   Tags

   10xHis and Twin-StrepTag

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPPI4-Strep-SNAP-CD28-GCN4-His6

   Plasmid

   #223585

   Purpose

   Protein purification of human CD28 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD28 (CD28 Human)
   

   Use

   Tags

   GCN4, His6 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPPI4-Strep-SNAP-CTLA-4-GCN4-His6

   Plasmid

   #223582

   Purpose

   Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CTLA4 (CTLA4 Human)
   

   Use

   Tags

   GCN4, His6 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPI4-Strep-SNAP-CD80 (I92R)-His10

   Plasmid

   #223580

   Purpose

   Protein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CD80 (CD80 Human)
   

   Use

   Tags

   His10 and Twin-Strep, SNAP

   Expression

   Mammalian

   Mutation

   I92R

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJS-BCD-Strep-SAVED-CHAT-cbm

    Plasmid

    #214032

    Purpose

    Bacterial expression plasmid for strep-tagged SAVED-CHAT cA3-binding pocket mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    K121E, Q122A, N274A, R276E, Y285A, F286A

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid

    #208625

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 72~197, other residues not included

    Promoter

    T7

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep

    Plasmid

    #213542

    Purpose

    AAV viral vector packaging plasmid that expresses CoV2

    Depositor

    Brandon Harvey, Christopher Richie

    Insert

    CoV2 Orf3a-2xStrep (ORF3a Severe acute respiratory syndrome coronavirus 2, Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOTTC1993 - pAAV EF1a CoV2 protE-2xStrep

    Plasmid

    #213543

    Purpose

    AAV viral vector packaging plasmid that expresses CoV2

    Depositor

    Brandon Harvey, Christopher Richie

    Insert

    CoV2 protE-2xStrep (E Severe acute respiratory syndrome coronavirus 2, Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIB-2STREP-HsHelz_1051-1942-GSSG-MBP_AG

    Plasmid

    #148795

    Purpose

    Mammalian Expression of HsHelz_1051-1942-GSSG

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    HsHelz_1051-1942-GSSG (HELZ Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTol2-F-actin-microtubule plus ends reporters

    Plasmid

    #196134

    Purpose

    zebrafish expression

    Depositor

    Reinhard Köster

    Insert

    F-actin-microtubule plus ends reporters
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNL(CMV)Rep78-III/CMV/WPREΔU3

    Plasmid

    #190726

    Purpose

    Expression of AAV2 Rep78-III in mammalian cells

    Depositor

    Jakob Reiser

    Article

    Li et al Hum Gene Ther Methods. 2018 Jun;29(3):13

    Insert

    AAV2 Rep78-III
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter

    Plasmid

    #172599

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-D842V+R914W (PDGFRA Synthetic, Human)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-D842V+R914W

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter

    Plasmid

    #172600

    Purpose

    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.

    Depositor

    Daniel Kiss

    Article

    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

    Insert

    myc-PDGFRA-K627R+R914W (PDGFRA Synthetic, Human)
    

    Use

    Egfp-pest_reporter

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    PDGFRA-K627R+R914W

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. human KANK2-A670V Ankyrin Repeats pET151

    Plasmid

    #164055

    Purpose

    Expresses human KANK2 Ank domain - A670V mutant in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 2 A670V mutant (KANK2 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mouse KANK4-Y801H Ankyrin Repeats pET151

    Plasmid

    #164058

    Purpose

    Expresses mouse KANK4 Ank domain -Y801H mutant in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 4 Y801H mutant (Kank4 Mouse)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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