We narrowed to 8,586 results for: Ott

1. pBSV2G_P0031-sgRNAflaB

   Plasmid

   #149630

   Purpose

   flaB gRNA expression in B. burgdorferi

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   sgRNAflaB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P0031

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBSV2_Psyn-sgRNAflaB

   Plasmid

   #149631

   Purpose

   flaB gRNA expression in B. burgdorferi

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   sgRNAflaB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBSV2_Psyn-sgRNAftsI

   Plasmid

   #149632

   Purpose

   ftsI gRNA expression in B. burgdorferi

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   sgRNAftsI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBSV2B_Psyn-sgRNA500

   Plasmid

   #149558

   Purpose

   gRNA expression in B. burgdorferi, parental vector

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBSV2G_Psyn-sgRNAflaB

   Plasmid

   #149560

   Purpose

   flaB gRNA expression in B. burgdorferi

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   sgRNAflaB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBSV2G_Psyn-sgRNAftsI

   Plasmid

   #149562

   Purpose

   ftsI gRNA expression in B. burgdorferi

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   sgRNAftsI
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBSV2G_Psyn-sgRNAmreB

   Plasmid

   #149566

   Purpose

   mreB gRNA expression in B. burgdorferi

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBSV2G_Psyn-sgRNArodA

   Plasmid

   #149568

   Purpose

   rodA gRNA expression in B. burgdorferi

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   sgRNArodA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Psyn

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. OA-986C

   Plasmid

   #125000

   Purpose

   Expresses dCas9-VPR driven by the bottleneck promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   dCas9-VPR
   

   Use

   Piggyback

   Tags

   Expression

   Insect

   Mutation

   Promoter

   bottleneck

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. OA-986E

    Plasmid

    #125002

    Purpose

    Expresses dCas9-VP64 driven by the bottleneck promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

    Insert

    dCas9-VP64
    

    Use

    Piggyback

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bottleneck

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p3E-Rab31

    Plasmid

    #109755

    Purpose

    Multisite gateway vector for 3' tagging with Rab31

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab31 (rab31 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p3E-Rab19

    Plasmid

    #109753

    Purpose

    Multisite gateway vector for 3' tagging with Rab19

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab19 (rab19 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3E-rab38a

    Plasmid

    #109699

    Purpose

    Multisite gateway vector for 3' tagging with rab38a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab38a (rab38a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p3E-rab32b

    Plasmid

    #109690

    Purpose

    Multisite gateway vector for 3' tagging with rab32b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab32b (rab32b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. actc1b-mKate2-rab39a

    Plasmid

    #109632

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab39a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab39a
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3E-EHD2a

    Plasmid

    #109568

    Purpose

    Multisite gateway vector for 3' tagging with EHD2a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    EHD2a
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p3E-CaaX(tK)

    Plasmid

    #109540

    Purpose

    Multisite gateway vector for 3' tagging with the CaaX domain from Kras

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CaaX domain from Kras
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-MBP1-K295TAG-W340A.pcDNA3-k

    Plasmid

    #126627

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site.

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon and W34…

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNIC28-BpWcbL

    Plasmid

    #125590

    Purpose

    Overexpression plasmid for production of WcbL from Burkholderia pseudomallei

    Depositor

    Nicholas Harmer

    Article

    Vivoli et al Chem Biol. 2015 Dec 17;22(12):1622-32. d

    Insert

    BpWcbL (wcbL Burkholderia pseudomallei)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAW218

    Plasmid

    #113240

    Purpose

    E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader IHF binding site flip

    Depositor

    Jennifer Doudna

    Article

    Wright et al Science. 2017 Sep 15;357(6356):1113-1118

    Inserts

    Cas1
    Cas2
    Leader-repeat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AAAAAATCATTAATTAATAATAGGTTATG->CATAACCTATTATTA…

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEHIShqprtK139A

    Plasmid

    #92403

    Purpose

    hQPRTase harbouring K139A mutation

    Depositor

    James Naismith, Huanting Liu

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    hQPRTase (QPRT Human)
    

    Use

    Tags

    TEV cleacable 6xHis

    Expression

    Bacterial

    Mutation

    K139A

    Promoter

    T7

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJBL-RNAOUTA4

    Plasmid

    #69458

    Purpose

    RNA-OUT A4 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-OUT A4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJBL-RNAINS4

    Plasmid

    #69456

    Purpose

    RNA-IN S4 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S4 + SFGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    mutations for S4 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJBL-RNAINS3

    Plasmid

    #69455

    Purpose

    RNA-IN S3 variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    mutations for S3 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJBL-RNAOUTA3

    Plasmid

    #69457

    Purpose

    RNA-OUT A3 variant - antisense (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-OUT A3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRII-Topo Tcrb-V13 in situ probe

    Plasmid

    #45622

    Depositor

    Jeffrey Macklis

    Article

    Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

    Insert

    Tcrb-V13 in situ probe (Tcrb Mouse)
    

    Use

    In situ

    Tags

    Expression

    Mutation

    fragment contains bp# 533-1058 of X67128

    Promoter

    Available since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

27. RCAS (A) Radical Fringe (CT#475)

    Plasmid

    #13981

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Radical Fringe (RFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

28. RCAS (B) Radical Fringe (CT#476)

    Plasmid

    #13982

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Radical Fringe (RFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

29. RCAS (B) flag Radical Fringe (CT#472)

    Plasmid

    #13983

    Depositor

    Cliff Tabin

    Article

    Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

    Insert

    Radical Fringe (RFNG Chicken)
    

    Use

    Retroviral; Avian expression

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

30. pmScarlet_peroxisome_C1

    Plasmid

    #85063

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Peroxisomal targeting sequence
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEIGW roGFP2-Orp1

    Plasmid

    #64993

    Purpose

    Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Lentiviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRSF-G1(6/7FTrp)RS

    Plasmid

    #207621

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPAmCherry-b-actin

    Plasmid

    #31949

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    b-actin

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GCaMP-AMPK-SPARK

    Plasmid

    #226257

    Purpose

    Dual reporter of Ca2+ levels and AMPK activity

    Depositor

    Roland Malli

    Article

    Erdogan et al ACS Sens. 2024 Aug 21. doi: 10.1021/acss

    Insert

    GCaMP-AMPK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pCAIP-hXRCC1wt-eGFP

    Plasmid

    #206032

    Purpose

    Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pmScarlet_NES_C1

    Plasmid

    #85060

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-Ii-OVA.I+II

    Plasmid

    #64596

    Purpose

    Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

    Depositor

    Martin Zenke, Sandra Diebold

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Invariant chain

    Expression

    Mammalian

    Mutation

    Only amino acids 242-353 (class I and class II ep…

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET15b-RvLEAMshort

    Plasmid

    #218122

    Purpose

    Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

    Depositor

    Ci Ji Lim

    Article

    Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

    Insert

    RvLEAMshort
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Truncated form (58-181aa)

    Promoter

    T7

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CS2 Flag-Smad2

    Plasmid

    #14042

    Depositor

    Joan Massague

    Article

    Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. 6xHis-SNAP-PI(4,5)P2 Probe

    Plasmid

    #211513

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    PLCdelta1-PH
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3-myc-CUL3

    Plasmid

    #19893

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    3xmyc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. eSpCas9(1.1)

    Plasmid

    #71814

    Purpose

    Expresses high specificity SpCas9. Px330-like plasmid.

    Depositor

    Feng Zhang

    Article

    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

    Has Service

    Cloning Grade DNA

    Insert

    enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tTRE, hPGK

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. 6xHis-SNAP-PI(4)P Probe

    Plasmid

    #211509

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

    Depositor

    Hannes Maib, David Murray

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    SidC (P4C)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pB-EF1a-NLuc-IRES-Puro

    Plasmid

    #130936

    Purpose

    PiggyBac vector for constitutive NanoLuc expression in mammalian cells

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    NanoLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLPCX roGFP2-Orp1

    Plasmid

    #64991

    Purpose

    Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

49. pET-MCN-10His-mStayGold (E138D)

    Plasmid

    #211361

    Purpose

    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

    Depositor

    Mohan Balasubramanian

    Article

    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

    Insert

    mStayGold
    

    Use

    Tags

    10xHis-tag

    Expression

    Bacterial

    Mutation

    E138D

    Promoter

    T7

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMTS-HA-GFP11

    Plasmid

    #177725

    Purpose

    Eleventh β-strand of GFP (GFP11) fused to a mitochondria signal sequence for mitochondrial targeting, HA tagged

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    MTS-HA-GFP11
    

    Use

    Tags

    GFP11 and HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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