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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,553 results for: pcDNA3.1+

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Showing: 3201 - 3250 of 5553 results

  15. NSarQ27G-VMAn-FKBP

    Plasmid
    #70230
    Purpose
    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pcDNA3-NGFP-VMAn-FKBP

    Plasmid
    #70225
    Purpose
    Expresses N-terminal portion of GFP along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    eGFP
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Glu 125 to Ile, Deleted amino acids 129-2…
    Promoter
    CMV
    Available Since
    Nov. 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. pcDNA-LBR-EGFP-polyA

    Plasmid
    #59748
    Purpose
    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
    Depositor
    Yi Zhang
    Article
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Insert
    LBR-EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    59748-plasmid-map-sequence-id-94052

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  35. pcDNA-LBR-mRFP1-polyA

    Plasmid
    #59749
    Purpose
    Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
    Depositor
    Yi Zhang
    Article
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Insert
    LBR-mRFP1
    Tags
    mRFP1
    Expression
    Mammalian
    Mutation
    aa 1-249 of LBR
    Promoter
    CMV and T7
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59749-plasmid-map-sequence-id-94053

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  38. mito-mGarnet

    Plasmid
    #104309
    Purpose
    codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito
    Depositor
    Ulrich Nienhaus
    Article
    Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103
    Insert
    mito-mGarnet
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    104309-plasmid-map-sequence-id-225634

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  40. mVenus-hCOL1A1

    Plasmid
    #140111
    Purpose
    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Insert
    Type I procollagen α1 (COL1A1 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    COL1A1 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available Since
    May 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140111-plasmid-map-sequence-id-296751

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Showing: 3201 - 3250 of 5553 results