20,039 results

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pcDNA3-CD68 promoter/enhancer

Plasmid

#34837

Depositor

Peter Murray

Article

Lang et al J Immunol. 2002 Apr 1;168(7):3402-11.

Insert

CD68 promoter/enhancer -680/+140 (CD68 Human)

Expression

Mammalian

Available Since

Jan. 18, 2012

Availability

Academic Institutions and Nonprofits only

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pGWB505

Plasmid

#74847

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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lenti_dCas9-KRAB-MeCP2

Plasmid

#122205

Purpose

Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Use

Lentiviral

Tags

KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 (D10A, H840A)

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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DP6

Plasmid

#140446

Purpose

Untargetted bacterial drift

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926 dam seqA emrR ugi CDA1 gIII

Expression

Bacterial

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-SRRM2-mCherry

Plasmid

#174089

Purpose

Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

Depositor

Wesley Sundquist, Katie Ullman

Article

Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

Insert

SRRM2 (SRRM2 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

TREtight

Available Since

Sept. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVSV-G

Plasmid

#138479

Purpose

Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

VSV-G envelop protein

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAG79-attL1-pegRNA-attL2

Plasmid

#213050

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1-GFP

Plasmid

#51407

Purpose

Is a biosensor for PI(4,5)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP

Plasmid

#86639

Purpose

Expresses mCherry-eGFP tandem (cytoplasmic)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

mCherry-EGFP

Tags

mCherry-EGFP

Expression

Mammalian

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pci-EGFP-UPF1-WT

Plasmid

#135994

Purpose

WT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-RLuc8

Plasmid

#87121

Purpose

Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.

Depositor

Sanjiv Sam Gambhir

Article

Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Mutation

A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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CROP-seq-opti

Plasmid

#106280

Purpose

A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.

Depositor

Jay Shendure

Article

Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

Insert

EF1a-Puro-WPRE-hU6-gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTet-IRES-EGFP

Plasmid

#64238

Purpose

Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

Depositor

Maria Lung

Article

Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

pTet

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-NbALFA

Plasmid

#159987

Purpose

For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mCherry-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mCherry and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-AID-BubR1

Plasmid

#47330

Purpose

for plasmid integration using the Flp-In system. AID at N-terminal

Depositor

Don Cleveland

Article

Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

Insert

BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)

Tags

AID and GFP

Expression

Mammalian

Mutation

siRNA resistant

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pPSU1

Plasmid

#89439

Purpose

High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders

Depositor

Song Tan

Article

Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.

Inserts

500 bp EcoRV fragment
1000 bp EcoRV fragment
1500 bp EcoRV fragment
2000 bp EcoRV fragment
500 bp PstI fragment
700 bp PstI fragment
800 bp PstI fragment (EHMT2 Human)
900 bp PstI fragment
1000 bp PstI fragment (BMI1 Human)
2000 bp PstI fragment

Expression

Bacterial

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBOB-VE-cadherin-GFP

Plasmid

#118732

Purpose

To express VE-cadherin tagged with GFP on C-terminus

Depositor

John Cooper

Article

Kim et al Mol Biol Cell. 2018 Jul 15;29(13):1693-1

Insert

Cadherin 5 (CD144) (CDH5 Human)

Use

Lentiviral

Tags

GFP

Mutation

I503T (Common SNP-see comments)

Promoter

CMV

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMV762-Peredox-mCherry

Plasmid

#90217

Purpose

Fluorescent reporter for NADH:NAD+ ratios in Mycobacteria.

Depositor

Ashwani Kumar

Article

Bhat et al Front Cell Infect Microbiol. 2016 Nov 8;

Insert

Codon Optimized Peredox-mCherry

Use

Shuttle vector

Expression

Bacterial

Promoter

groEL

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO SV40 small + Large T (w612-1)

Plasmid

#22298

Depositor

Eric Campeau

Insert

SV40 small + Large T antigen

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pMK243 (Tet-OsTIR1-PURO)

Plasmid

#72835

Purpose

Expresses OsTIR1 under the control of the Tet promoter

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

AAVS1-Tet-OsTIR1-PURO-AAVS1

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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