We narrowed to 11,193 results for: ENA
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Plasmid#205002PurposeThe vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.DepositorInsertmCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
UseTardigrade expressionAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJMP1171
Plasmid#119253Purposerfp "test" strainDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1170
Plasmid#119252Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK4-S
Plasmid#172425PurposeExpression of full-length, human GIRK4 with C-terminal StrepTag IIDepositorAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH2523
Plasmid#191358PurposePunc-25-GCaMP3::UrSL2 ::wCherry unc-54 3' UTR C.elegans DD neurou expression of GCaMP3 RFPDepositorInsertGCaMP3
TagsmCherryExpressionWormPromoterPunc-25Available SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109HgAmp30E
Plasmid#123149PurposeMercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerT, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
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Delta DED FLIP-L
Plasmid#13790DepositorInsertDELTA DED FLIP LONG (CFLAR Human)
Tags8 x His tagExpressionBacterialMutationDelta DED removes first 186 residuesAvailable SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
SauCas9 EMX1-nicking sgRNA
Plasmid#169860PurposeSauCas9 nicking sgRNA for EMX1DepositorInsertSauCas9 EMX1-nicking sgRNA
ExpressionMammalianPromoterhuman U6 promoterAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
G1397 DddAtox-C–dSpCas9
Plasmid#157836Purposeexpresses split DddAtox-Cas9 construct in mammalian cellsDepositorInsertbpNLS–G1397 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
UseCRISPRAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-ADAM29i1
Plasmid#59606PurposeExpression of shRNA against human ADAM29DepositorAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
Plasmid#125225PurposeExpresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cellsDepositorInsertgLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianPromoterCMVAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-sfGFP
Plasmid#114598Purpose"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisPromoterPBenAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisPromoterOR2-OR1-PrAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB11 (pDEST-DHFR F[1,2] C-term, NatR)
Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB12 (pDEST-DHFR F[3] C-term, HygR)
Plasmid#210486PurposepDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…ExpressionYeastPromoterADH1Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQCas12j2
Plasmid#189780PurposeGateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoterDepositorInsertCas12j2
UseCRISPR; Gateway compatible cas12j2 entry cloneTagsNLSExpressionPlantAvailable SinceOct. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SHMT1
Plasmid#106304PurposeExpress SHMT1DepositorAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPKm-202
Plasmid#90492PurposeExpresses pcDNA3 - CMVmin - 5X UAS - pFR - CMVmin LuciferaseDepositorInsertCMVmin Luciferase Fluc
ExpressionMammalianAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-LbCas12a-Plus-mCherry
Plasmid#191230PurposeTo express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificityDepositorInsertLbCas12a-Plus-P2A-mCherry
UseCRISPRMutationD156R, R883K, R887AAvailable SinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-Gateway
Plasmid#114011Purpose3rd generation lentiviral empty sgRNA expression vector with a Gateway cassette for co-expression of a reporter cDNADepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0601
Plasmid#91016PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_D10A nickase
UseCRISPRMutationD10APromoter35SAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Bongwoori
Plasmid#63720PurposeConsists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227DDepositorInsertBongwoori
TagsSuper ecliptic pHlorin A227DExpressionMammalianPromoterCMVAvailable SinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Wnt3a (CT#169)
Plasmid#13976DepositorInsertWnt3a (Wnt3a Mouse)
UseRetroviral; Avian expressionAvailable SinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSilencer2.1-U6-4JC1RR
Plasmid#190847PurposeFor mammalian expression of 4JC1RR, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.DepositorInsert4JC1RR (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyExpressionMammalianPromoterU6Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSilencer2.1-U6-NL1F50
Plasmid#190831PurposeFor mammalian expression of NL1F50, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a flexible linker.DepositorInsertNL1F50 (A Dual-Specificity aptamer targeting OGT and GFP)
UseAffinity Reagent/ Antibody and Synthetic BiologyExpressionMammalianPromoterU6Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
J23103_GB
Plasmid#65991PurposeMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23107_GB
Plasmid#65999PurposeMoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23116_GB
Plasmid#66003PurposeMoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A
Plasmid#83483PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477AAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds
Plasmid#119074PurposeZebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporterDepositorInsertubb:MCP-p65-HSF1-2a-Cerulean
ExpressionBacterialPromoterZebrafish ubiquitin promoterAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds
Plasmid#119075PurposeZebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporterDepositorInsertubb:Com-KRAB-2a-Cerulean
ExpressionBacterialPromoterZebrafish ubiquitin promoterAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-com-Ds
Plasmid#119077PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loopsDepositorInsertU6a:2xCom-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-MS2-Ds
Plasmid#119076PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loopsDepositorInsertU6a:2xMS2-sgRNA
ExpressionBacterialPromoterZebrafish U6a promoterAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
YEpURA-CUP1-HBTubiquitin
Plasmid#26900DepositorInsertHBT-tagged ubiquitin
ExpressionYeastAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
Plasmid#125230PurposeFor generating SPARK NanoLuc-oβ2AR transmembrane component lentivirusDepositorInsertgLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
UseLentiviralTagsHA,FlagExpressionMammalianPromoterCMVAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-mCherry-N
Plasmid#210271PurposeN-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.DepositorTypeEmpty backboneTagsmCherryExpressionMammalianAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_791
Plasmid#216079PurposeMinimal plasmid; contains only ori and ampR, destination vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseDestinationExpressionBacterialAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA295
Plasmid#215986PurposeFragmid fragment: (N' terminus) SV40 Nuclear Localization SignalDepositorHas ServiceCloning Grade DNAInsertStart; SV40NLS_v1.19
UseFragmentAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA014
Plasmid#216057PurposeFragmid fragment: (2A Selection) Puromycin resistanceDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; PuroR_v1.1; Stop
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA163
Plasmid#216061PurposeFragmid fragment: (2A Selection) Stop onlyDepositorHas ServiceCloning Grade DNAInsertStop
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA065
Plasmid#215965PurposeFragmid fragment: (Pol 2 promoter) human EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEF1a_v1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2813
Plasmid#91092PurposeModule C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorInsertCsy4 (+ BaeI site for GT donor cloning)
UseCRISPRPromoterPvUbi2Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1p shDna2
Plasmid#31951DepositorAvailable SinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJMP1217
Plasmid#119258Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEY22
Plasmid#191028Purposelim-4 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorAvailable SinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEY16
Plasmid#191022Purposeflp-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)DepositorAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only