19,614 results

1. pKD236-4b

   Plasmid

   #90956

   Purpose

   Expresses ThsS constitutively

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Insert

   ThsS
   

   Expression

   Bacterial

   Promoter

   Bba_J23104

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. EF1a-Cas9-2XNES-2ERT2

   Plasmid

   #120551

   Purpose

   Express Cas9, 2xNES and 2 tandem ERT2 in mammalian cells

   Depositor

   Yu Wang

   Article

   Zhao et al Mol Ther Nucleic Acids. 2018 Dec 7;13:20

   Insert

   Cas9, NES and ERT2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSET FLIPPi-200u

   Plasmid

   #13555

   Depositor

   Wolf Frommer

   Article

   Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

   Insert

   FLIPPi G162A
   

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   G162A in binding protein

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO.1-puro U6 sgRNA BfuAI large stuffer

   Plasmid

   #52628

   Purpose

   lentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiency

   Depositor

   Scot Wolfe

   Insert

   Kanamycin cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHRm-GFP(1-10)-VP64-NLS

   Plasmid

   #70228

   Purpose

   Expresses GFP(1-10)-VP64-NLS in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP(1-10)-VP64-NLS
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKD237-3a-2

   Plasmid

   #90957

   Purpose

   Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Thiosulfate response regulator
   Superfolder GFP
   mCherry
   

   Expression

   Bacterial

   Promoter

   Bba_J23100, Bba_J23105, and PphsA

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV/D377Y-mPCSK9

   Plasmid

   #58376

   Purpose

   Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Has Service

   AAV8

   Insert

   proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   D377Y

   Promoter

   HCRApoE/hAAT

   Available Since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPGKpuro

   Plasmid

   #11349

   Depositor

   Rudolf Jaenisch

   Article

   Tucker et al Genes Dev. 1996 Apr 15. 10(8):1008-20.

   Insert

   pgk-1 5' and 3' regions (Pgk1 Mouse)
   

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTH100

   Plasmid

   #84458

   Purpose

   pJB38-NWMN29-30 + SarA_P1-sGFP-Term

   Depositor

   Reindert Nijland

   Article

   de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

   Insert

   SarA_P1-sGFP from pCM29
   

   Tags

   6xHis (on sfGFP)

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA6 asyn E46K

    Plasmid

    #105730

    Purpose

    Mammalian expression of mutant human alpha synuclein

    Depositor

    Hilal Lashuel

    Article

    Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

    Insert

    alpha-synuclein E46K (SNCA Human)
    

    Expression

    Mammalian

    Mutation

    E46K

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiV_Cas9_puro

    Plasmid

    #108100

    Purpose

    Lentiviral expression plasmid of spCas9 with puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS promoter

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. OCT4-2A-eGFP-PGK-Puro

    Plasmid

    #31938

    Purpose

    Human OCT4 genomic targeting donor

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Targeting donor

    Tags

    eGFP

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. -1748 human cyclin D1 promoter pGL3Basic

    Plasmid

    #32726

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase; Reporter gene

    Promoter

    -1748CCND1 promoter

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LV-AR

    Plasmid

    #213819

    Purpose

    Lenti-viral expression of human androgen receptor wild-type (WT).

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR (human androgen receptor) (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    No, it is AR wild-type.

    Promoter

    EF-1a

    Available Since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEM705 Gs

    Plasmid

    #109350

    Purpose

    Wildtype Gαs

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    GαS (GNAS Human)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-GFP

    Plasmid

    #37825

    Depositor

    Edward Boyden

    Has Service

    AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDR196

    Plasmid

    #36029

    Depositor

    Dominique Loque, Wolf Frommer, Doris Rentsch

    Article

    Meyer et al J Biol Chem. 2006 Mar 17;281(11):7197-20

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7 RNA POL

    Plasmid

    #138352

    Purpose

    Expressing T7 RNA Polymerase in mammalian cells

    Depositor

    Baisong Lu

    Insert

    T7 RNA POL
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-ATF6

    Plasmid

    #32955

    Purpose

    For visualization of GFP-ATF6 in mammalian cells.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    M67L and M313I

    Promoter

    CMV

    Available Since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKM200

    Plasmid

    #13079

    Depositor

    Kenan Murphy

    Article

    Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

    Insert

    Ptac-gam-bet-exo operon
    

    Expression

    Bacterial

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge