162,676 results

1. pEGFP-C1 Lifeact-EGFP

   Plasmid

   #58470

   Purpose

   Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   Lifeact
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-alphasynuclein-A53T

   Plasmid

   #40823

   Depositor

   David Rubinsztein

   Article

   Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

   Insert

   SNCA (SNCA Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A53T (aggregates more rapidly than the wild-type …

   Promoter

   CMV

   Available since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInducer20-Blast

   Plasmid

   #109334

   Purpose

   pInducer20 with added blasticidin resistance

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Type

   Empty backbone

   Use

   Lentiviral; Inducible expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOxyR_gfp

   Plasmid

   #194154

   Purpose

   Green Fluorescent Reporter of intracellular hydrogen peroxide

   Depositor

   Sven Panke

   Article

   Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

   Insert

   transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PoxyR, PoxyS

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB_TRE3G_scFV-sfGFP-Ezh2FL

   Plasmid

   #235575

   Purpose

   Dox-inducible expression of epigenetic effector Ezh2 fused with scFV to programme H3K27me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Ezh2 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRT043-CLYBL-DDdcas9-VPH

   Plasmid

   #158091

   Purpose

   Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

   Depositor

   Martin Kampmann

   Article

   Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

   Insert

   CLYBL-CAG-DDdCas9VPH-T2A-GFP
   

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZS2oxySp-GFP-proD-oxyR

   Plasmid

   #78224

   Purpose

   synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

   Insert

   synthetic gene circuit
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOxyR_rfp

   Plasmid

   #194155

   Purpose

   Red Fluorescent Reporter of intracellular hydrogen peroxide

   Depositor

   Sven Panke

   Article

   Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

   Insert

   transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PoxyR, PoxyS

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-IRES-Blast

    Plasmid

    #85133

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSPObooster

    Plasmid

    #216160

    Purpose

    Plasmid expressing the RME1(ins-308A) and MKT1(30G) genes from S. cerevisiae to correct sporulation defect of S288C derived yeast lab strains

    Depositor

    Grant Brown

    Article

    Loll-Krippleber et al Yeast. 2024 Sep 9. doi: 10.1002/yea.3978

    Inserts

    MKT1(30G) (MKT1 Budding Yeast)
    RME1(ins-308A) (RME1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    MKT1 gene containing an aspartic acid to glycine …

    Promoter

    endogenous promoter

    Available since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a_6H-MMLV_RT_D524N-6H

    Plasmid

    #166945

    Purpose

    Bacterial expression of M-MLV reverse transcriptase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    gag-pol
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D524N, H8Y

    Promoter

    T7

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDisplay-BirA-ER

    Plasmid

    #20856

    Depositor

    Alice Ting

    Article

    Howarth et al Nat Methods. 2008 May . 5(5):397-9.

    Insert

    BirA-ER
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C-terminal ER targeting sequence (KDEL)

    Promoter

    Available since

    May 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST-CMV-3xFLAG-gateway-EGFP

    Plasmid

    #122845

    Purpose

    N-terminal 3xFLAG tag and C-terminal EGFP tag. Gateway destination vector for mammalian expression.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P1-GFP-Nanobody

    Plasmid

    #61838

    Purpose

    Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    GFP-Nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T1 SHP2 WT

    Plasmid

    #8322

    Depositor

    Ben Neel

    Article

    O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-loxPSTOPloxP-ZsGreen

    Plasmid

    #51269

    Purpose

    Cre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    loxP-STOP-loxP-ZsGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-BAZ1B

    Plasmid

    #65372

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BAZ1B (BAZ1B Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMitoTimer

    Plasmid

    #52659

    Purpose

    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

    Depositor

    Zhen Yan

    Article

    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

    Insert

    DsRed1-E5
    

    Use

    Dsred

    Tags

    human cytochrome c oxidase subunit VIII mitochond…

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. C1(1-29)-TurboID-V5_pCDNA3

    Plasmid

    #107173

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    Available since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge