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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#71237

Purpose

Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

Depositor

Charles Gersbach

Publication

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A GFP
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

66 Articles

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pCAG-mGFP

Plasmid

#14757

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Has service

Cloning Grade DNA

Insert

membrane bound form of EGFP

Tags

Palmitoylation signal

Expression

Mammalian

Promoter

pCAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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pSico

Plasmid

#11578

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Publication

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Has service

Cloning Grade DNA

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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HRE-luciferase

Plasmid

#26731

Purpose

Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

Depositor

Navdeep Chandel

Publication

Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

Insert

Hypoxia Responsive Elements (3)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

90 Articles

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pLJC5-Tmem192-3xHA

Plasmid

#102930

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-HA)

Depositor

David Sabatini

Publication

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

HA

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

65 Articles

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pCMV-PEmax-P2A-hMLH1dn

Plasmid

#174828

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-hMLH1dn

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript; deletion of residues 754-…

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

28 Articles

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myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Publication

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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HA GSK3 beta S9A pcDNA3

Plasmid

#14754

Purpose

Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

Depositor

Jim Woodgett

Publication

Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

Insert

GSK3 beta S9A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A. Constitutively active mutant.

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

68 Articles

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FHRE-Luc

Plasmid

#1789

Purpose

Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

Depositor

Michael Greenberg

Publication

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FHRE (FOXO3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

3 copies of FRE

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

48 Articles

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1036 pcDNA3 Myr HA Akt1

Plasmid

#9008

Purpose

Mammalian expression of human Akt1 with myr, HA tags

Depositor

William Sellers

Publication

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

77 Articles

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pcDNA3-S28 histone 3 phosphorylation reporter-NLS

Plasmid

#32787

Depositor

Alice Ting

Publication

Lin et al Angew Chem Int Ed Engl. 2004 May 24;43(2

Insert

CFP-14-3-3tau-H3 peptide-YFP

Tags

CFP and YFP

Expression

Mammalian

Available since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only
CMV-R-GECO1

Plasmid

#32444

Purpose

Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Publication

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Expression

Mammalian

Mutation

Substitutions relative to the mApple-derived anal…

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

63 Articles

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Publication

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

34 Articles

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pcDNA3-EGFP-RhoA-Q63L

Plasmid

#12968

Depositor

Gary Bokoch

Publication

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA constitutively active (RHOA Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q63L Constitutively active

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

48 Articles

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mCh-alpha-tubulin

Plasmid

#49149

Purpose

expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

Depositor

Gia Voeltz

Publication

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

alpha tubulin (TUBA1B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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GFP-Nucleolin

Plasmid

#28176

Depositor

Michael Kastan

Publication

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

Nucleolin (NCL Human)

Tags

GFP

Expression

Mammalian

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pEGFP-C3-hYAP1

Plasmid

#17843

Depositor

Marius Sudol

Publication

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

36 Articles

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piRFP

Plasmid

#31857

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Publication

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Has service

Cloning Grade DNA

Insert

iRFP

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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pcDNA3 MDM2 WT

Plasmid

#16233

Depositor

Mien-Chie Hung

Publication

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Expression

Mammalian

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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