We narrowed to 4,881 results for: phy

1. pET21c(+)-mtLeuRS

   Plasmid

   #31079

   Depositor

   Linda Spremulli

   Article

   Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

   Insert

   Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-HsSENP3

   Plasmid

   #71467

   Purpose

   Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCW57.1-Luciferase

   Plasmid

   #99283

   Purpose

   All-in-one lentivector with tet-inducible luciferase

   Depositor

   Stephen Tapscott

   Article

   Shadle et al PLoS Genet. 2017 Mar 8;13(3):e1006658. d

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQmod3C-GG

   Plasmid

   #191350

   Purpose

   Clostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Plac-RFP (IPTG-inducible red fluorescent protein)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-TDsmURFP-IRES-eGFP

   Plasmid

   #80344

   Purpose

   Expresses Tandem Dimer smURFP and eGFP in mammalian cells.

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and IRES

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHAGE-IRES-puro-NLS-dPguCas13b-3xEGFP-NLS-3xFlag

   Plasmid

   #132399

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKPI-MtLAP1

   Plasmid

   #193521

   Purpose

   Anthocyanin pigmentation based visual marker for arbuscular mycorrhizal fungal colonization of Medicago truncatula roots

   Depositor

   Jeremy Murray

   Article

   Kumar et al New Phytol. 2022 Sep 20. doi: 10.1111/np

   Inserts

   MtLAP1
   DsRed
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ATUBI10 and Medtr8g059790(KPI)

   Available since

   June 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCI-neo:alpha4-mcherry

   Plasmid

   #45073

   Depositor

   Henry Lester

   Article

   Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

   Insert

   alpha4-mcherry (Chrna4 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132400

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19-Mu-switch-R-loop

    Plasmid

    #134899

    Purpose

    Generation and purification of R-loops for in vitro biochemistry

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    mouse IgH switch repeat region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNW-sfGFP(SPS6)

    Plasmid

    #133814

    Purpose

    Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    sfGFP(SPS6)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ppta

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. a1Ic-HE3-pcDNA3

    Plasmid

    #45810

    Depositor

    Edward Perez-Reyes

    Article

    Gomora et al Biophys J. 2002 Jul;83(1):229-41.

    Insert

    CACNA1I (CACNA1I Human)
    

    Use

    Making crna for oocyte injections

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR-ARF19

    Plasmid

    #83846

    Purpose

    Gateway donor vector for ARF19

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    ARF19 (ARF19 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSc-humanADAR2

    Plasmid

    #171106

    Purpose

    Expresses human ADAR2 in yeast.

    Depositor

    Brenda Bass

    Article

    Macbeth et al Methods Enzymol. 2007;424:319-31. doi: 1

    Insert

    human ADAR2 (ADARB1 Human)
    

    Use

    Tags

    His12_TEV

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA1-GATA6

    Plasmid

    #51929

    Purpose

    mammalian expression of mouse GATA6

    Depositor

    Jeffery Molkentin

    Article

    Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

    Insert

    GATA6 (Gata6 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pEGB 35S:YFP:Tnos (GB0209)

    Plasmid

    #68219

    Purpose

    Transcriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    YFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MAC_C_TYRO3

    Plasmid

    #187793

    Purpose

    MAC-tagged gene expression of human TYRO3

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    TYRO3 (TYRO3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMB-SaCas9

    Plasmid

    #105998

    Purpose

    SaCas9 expression vector

    Depositor

    Mike McManus

    Article

    Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

    Insert

    Cas9 nuclease
    

    Use

    CRISPR and Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    short EF1alpha

    Available since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-C1-Nup35-mut

    Plasmid

    #87341

    Purpose

    To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP35 (NUP35 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTYF-super-mfCCK-0.5k-Venus-WPRE

    Plasmid

    #156392

    Purpose

    Expresses Venus under CCK gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. LZF97_hSyn-spine-jRGECO1a

    Plasmid

    #119198

    Purpose

    Targets jRGECO1a to spines in living neurons.

    Depositor

    Don Arnold

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    jRGECO1a-TPR3-Utrophin(1-78)-TPR3-Zfn(CCR5-L) (Utrn Synthetic, Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin 1

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKESaPar

    Plasmid

    #211829

    Purpose

    Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. SadCas9 VPR

    Plasmid

    #188514

    Purpose

    Expresses FLAG tagged SadCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKERepPar

    Plasmid

    #211827

    Purpose

    Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKCcas9dO

    Plasmid

    #62552

    Purpose

    High efficiency Streptomyces genome editing by CRISPR/Cas9 system

    Depositor

    Weihong Jiang

    Article

    Huang et al Acta Biochim Biophys Sin (Shanghai). 201

    Inserts

    Scocas9
    sgRNA ()
    act-orf4 homology arm
    

    Use

    CRISPR; Bacterial genome editing

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    j23119 and ptipA

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pQmod2C-GG

    Plasmid

    #191347

    Purpose

    Clostridium expression vector (pBP1 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SL016: AAV-CAG-FLEX-CheRiff-GFP

    Plasmid

    #124776

    Purpose

    For AAV based expression of CheRiff in Cre-Positive cells

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    CheRiff-GFP
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

28. SadCas9 KRAB

    Plasmid

    #188504

    Purpose

    Expresses FLAG tagged SadCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/N580A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MAC_C_EphA7

    Plasmid

    #187765

    Purpose

    MAC-tagged gene expression of human EphA7

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EphA7 (EPHA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFL-EGFP-3xFlag-DEST

    Plasmid

    #134903

    Purpose

    Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Type

    Empty backbone

    Use

    Tags

    3x-Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3.1+-PAFAH1B1

    Plasmid

    #92430

    Purpose

    mammalian expression of PAFAH1B1

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BA31_pU.CAG.SaCas9-D10A.rBGpA

    Plasmid

    #199251

    Purpose

    Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SaCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3/Myc-DNMT3A2

    Plasmid

    #36941

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3A2 (DNMT3A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MAC_C_EPHB2

    Plasmid

    #187797

    Purpose

    MAC-tagged gene expression of human EPHB2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EPHB2 (EPHB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pALOD4 non-binding mutant

    Plasmid

    #111027

    Purpose

    Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

    Depositor

    Arun Radhakrishnan

    Article

    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

    Insert

    Domain 4 of Anthrolysin O
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: S404C, C472A, G501A, T502A, T503A, L5…

    Promoter

    T7

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MAC_C_MUSK

    Plasmid

    #187781

    Purpose

    MAC-tagged gene expression of human MUSK

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    MUSK (MUSK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHDE-35S-Cas9-mCherry

    Plasmid

    #78931

    Purpose

    For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants

    Depositor

    Yunde Zhao

    Article

    Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pATmin-GG

    Plasmid

    #191352

    Purpose

    Clostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mEos2-Mito-7

    Plasmid

    #57401

    Purpose

    Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits