We narrowed to 6,480 results for: poly

1. pCAGGS_VP30_EBOV

   Plasmid

   #103051

   Purpose

   Encodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   VP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYFP-RPB1aAmr

   Plasmid

   #75284

   Purpose

   Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cells

   Depositor

   Xavier Darzacq, Robert Singer

   Article

   Darzacq et al Nat Struct Mol Biol. 2007 Sep;14(9):796-

   Insert

   POLR2A (POLR2A Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   N792D alpha-amanitin–resistant

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti CMVie-IRES-BlastR

   Plasmid

   #119863

   Purpose

   3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

   Depositor

   Ghassan Mouneimne

   Article

   Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pOPINB-AMSH*

   Plasmid

   #66712

   Purpose

   Expresses activated AMSH in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Inserts

   hbp (Stam2 Mouse)
   AMSH (STAMBP Human)
   

   Use

   Tags

   His-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. gRNA-trp-HYB

   Plasmid

   #64331

   Purpose

   trp1 deletion gRNA cassette carried by pRS42H

   Depositor

   Yong-Su Jin

   Article

   Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

   Insert

   gBlock product of trp1 deletion gRNA cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCMVTAG-NEMO

   Plasmid

   #11970

   Depositor

   Jon Ashwell

   Article

   Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

   Insert

   NEMO (IKBKG Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV6-AN-HA_WT REV1

   Plasmid

   #201586

   Purpose

   Expresses human DNA repair protein REV1 with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA repair protein REV1 (REV1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JT113-pETDuet1-(R)-hREV3L

   Plasmid

   #64872

   Purpose

   bacterial expression of human REV3L

   Depositor

   Richard Wood

   Article

   Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

   Insert

   REV3L (REV3L Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOG-Cre

   Plasmid

   #233265

   Purpose

   Plasmid expressing Cre recombinase that is used to insert DNA CARGO into RMCE homing locus in Rosa26

   Depositor

   Mauro Calabrese

   Article

   Trotman et al Mol Cell. 2025 Mar 20;85(6):1117-1133.e1

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLJM1-Flag-Rheb

    Plasmid

    #165031

    Purpose

    expression of Flag tagged Rheb protein in mammalian cells

    Depositor

    Ken Inoki

    Article

    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d

    Insert

    Rheb (RHEB Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. gRNA-ura-HYB

    Plasmid

    #64330

    Purpose

    ura3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of ura3 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Flag-ATXN1

    Plasmid

    #48189

    Purpose

    Expresses Ataxin1

    Depositor

    Huda Zoghbi

    Article

    Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

    Insert

    Ataxin1 (ATXN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTSara-C

    Plasmid

    #60722

    Purpose

    Expresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    Residues 180-880 of T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Only amino acids 180-880 of T7 RNAP

    Promoter

    PBAD

    Available since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTSara-N

    Plasmid

    #60721

    Purpose

    Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    Residues 1-179 of T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Only amino acids 1-179 of T7 RNAP

    Promoter

    PBAD

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GAL FLAG103QDProGFP p306

    Plasmid

    #15834

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

16. GAL FLAG103QDProGFPp303

    Plasmid

    #15587

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 103Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pLyGo-Ec-8

    Plasmid

    #163136

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P(rhaB)

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GAL FLAG72QDProGFP p306

    Plasmid

    #15833

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 72Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pOPINB-OTUB1*

    Plasmid

    #65441

    Purpose

    Expresses activated OTUB1 in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Inserts

    UbcH5b (UBE2D2 Human)
    otubain-1 (OTUB1 )
    

    Use

    Tags

    His-3C

    Expression

    Bacterial

    Mutation

    Active site cysteine mutated to alanine

    Promoter

    T7

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

20. GAL FLAG25QDProGFP p306

    Plasmid

    #15831

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Oct. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pPRIME-TET-GFP-FF3

    Plasmid

    #11662

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a tetracycline-responsive promoter (TET) controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Myo1E-pEGFP

    Plasmid

    #124658

    Purpose

    Mouse myosin-1e fused with EGFP

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    Myo1e (Myo1e Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPRIME-CMV-GFP-FF3

    Plasmid

    #11663

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with CMV promoter controlling expression of GFP and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-ELL pBacPAK8

    Plasmid

    #15322

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    eleven nineteen lysine rich in leukemia (ELL Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pYLL201

    Plasmid

    #53454

    Purpose

    Expresses HA-tagged mouse DNA topoisomerase IIbeta (full length cDNA, in pCS2+ backbone)

    Depositor

    Leroy Liu, Lisa Lyu

    Article

    Ban et al Mol Cell Biol. 2013 Oct;33(20):4008-16.

    Insert

    HA-tagged Mus musculus topoisomerase (DNA) II beta (Top2b) (Top2b Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTRY-GFP

    Plasmid

    #15301

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    hrGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pCMV-Neo-Bam E2F6

    Plasmid

    #37965

    Depositor

    Jacqueline Lees

    Article

    Trimarchi et al Proc Natl Acad Sci U S A. 2001 Feb 13;98

    Insert

    E2F6 (E2F6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLyGo-Kp-1

    Plasmid

    #163143

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCPP6450

    Plasmid

    #128731

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

    Insert

    HopAD1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pVETL-Cmcs-Flag-Ataxin3Q80

    Plasmid

    #22120

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pVETL-Cmcs-Flag-Ataxin3Q25

    Plasmid

    #22118

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

32. pVenus-N-SLC35B2

    Plasmid

    #155038

    Purpose

    Transient expression of SLC35B2-Venus

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV

    Available since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. GAL 103Q+ProGFPp416

    Plasmid

    #15583

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt103Q + Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

34. pET19b-EcMscL-TEV-mEGFP

    Plasmid

    #165097

    Purpose

    Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

    Depositor

    Neha Kamat

    Article

    Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

    Insert

    E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
    

    Use

    Synthetic Biology

    Tags

    monomeric Enhanced Green Fluorescent Protein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKP3915

    Plasmid

    #113335

    Purpose

    Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Insert

    Artificial Transcription Factor (McIsaac et al, 2013)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBMN-AS-HNF4α

    Plasmid

    #139305

    Purpose

    Knocking down of human HNF4α through shRNA and amiRNA targeting HNF4α

    Depositor

    Claes Wadelius

    Article

    Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

    Insert

    shRNA and amiRNA against HNF4α
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. gRNA-leu-HYB

    Plasmid

    #64332

    Purpose

    leu2 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of leu2 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pDEST26-USP47

    Plasmid

    #64326

    Purpose

    Mammalian expression and purification of USP47.

    Depositor

    Grigory Dianov

    Article

    Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

    Insert

    USP47 (USP47 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. gRNA-his-HYB

    Plasmid

    #64333

    Purpose

    his3 deletion gRNA cassette carried by pRS42H

    Depositor

    Yong-Su Jin

    Article

    Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

    Insert

    gBlock product of his3 deletion gRNA cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pOPINS-AREL1

    Plasmid

    #66710

    Purpose

    Expresses AREL1 HECT domain in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    KIAA0317 (AREL1 )
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

41. p5M-hPR-B

    Plasmid

    #89114

    Purpose

    mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-B (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 restriction sites deleted in polylinker

    Promoter

    CMV

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pGEX6P1-optimizedNedd4.2d

    Plasmid

    #165103

    Purpose

    bacterial expression of a GST fusion of Human Nedd4.2d

    Depositor

    Arthur Haas

    Article

    Todaro et al J Biol Chem. 2017 Nov 24;292(47):19521-1

    Insert

    Nedd4.2d (NEDD4L Human)
    

    Use

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    codon optimized for E. coli expression

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAOJ15

    Plasmid

    #91567

    Purpose

    Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

    Depositor

    Harry Mobley

    Article

    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tetracycline promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. -227 hTF

    Plasmid

    #15442

    Depositor

    Nigel Mackman

    Article

    Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

    Insert

    Tissue factor promoter (F3 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

45. pCAP03-ermE*p

    Plasmid

    #120234

    Purpose

    Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring.

    Depositor

    Bradley Moore

    Article

    Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

    Insert

    promoter cassette containing ermE*p promoter and ampicillin resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    bla (amp resistance gene)

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. LentiV_Neo_Sik3

    Plasmid

    #119972

    Purpose

    Lentiviral expression plasmid of mouse Sik3 cDNA with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    Sik3 (Sik3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pIC306

    Plasmid

    #52257

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska3 (SKA3 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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48. pPRIME-CMV-dsRed-FF3

    Plasmid

    #11664

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of dsRed and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

49. pMALcPP-MS2cp

    Plasmid

    #203351

    Purpose

    Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

    Depositor

    Thomas Cech

    Article

    Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

    Insert

    MS2 coat protein
    

    Use

    Tags

    Hx6-MBP

    Expression

    Bacterial

    Mutation

    V75E and A81G, to prevent capsid assembly

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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50. NylB'-SCY_pET21b

    Plasmid

    #215417

    Purpose

    Expression construct for NylB'-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB'-SCY
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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