We narrowed to 22,307 results for: CRISPR

1. pRU320

   Plasmid

   #167682

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUBQ4-2::asCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6_(GLuc)_sgRNA

   Plasmid

   #68422

   Purpose

   Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   Gluc sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p426-SNR52p-gRNA.clr-2.Y-SUP4t

   Plasmid

   #89692

   Purpose

   gRNA for clr-2 locus

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gRNA for clr-2 locus
   

   Use

   CRISPR; Grna

   Tags

   No

   Expression

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS316-RGR-GFP-mHDV

   Plasmid

   #51058

   Purpose

   Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Yeast ADH1 promoter

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS316-RGR-GFP-mHH

   Plasmid

   #51057

   Purpose

   Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Yeast ADH1 promoter

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Mesp-1916/-1>nls::Cas9::nls

   Plasmid

   #59988

   Purpose

   Mesp driver driving nls::Cas9::nls

   Depositor

   Lionel Christiaen

   Article

   Stolfi et al Development. 2014 Nov;141(21):4115-20. d

   Insert

   nls::Cas9::nls
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Reverted mutations from dCas9 to wiltdtype

   Promoter

   Ciinte.Mesp -1916/-1

   Available since

   Nov. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-H11r1-2-DD-Cas9

   Plasmid

   #85978

   Purpose

   Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ158

   Plasmid

   #69307

   Purpose

   Gateway entry vector with hSpCas9 (Human codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   hSpCas9 (Human codon optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   NLS fusion

   Promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA EMX1

   Plasmid

   #128121

   Purpose

   Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

   Depositor

   Dominik Niopek

   Article

   Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

   Insert

   sgRNA EMX1 + GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV/U6

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330A_FokI-1x6

    Plasmid

    #63606

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX458_ZNF331_1

    Plasmid

    #135739

    Purpose

    Encodes gRNA for 3' target of human ZNF331

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF331 gRNA (ZNF331 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX458_ZNF331_2

    Plasmid

    #135740

    Purpose

    Encodes gRNA for 3' target of human ZNF331

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF331 gRNA (ZNF331 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF820_U6-sgRNA-EF1a-mCherry2_lenti

    Plasmid

    #138316

    Purpose

    Lenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFP

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    SpyCas9 GFP sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSB1C3-hsg(M)

    Plasmid

    #138262

    Purpose

    Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

    Insert

    sgRNA M
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458_SAP130_1

    Plasmid

    #86310

    Purpose

    Encodes gRNA for 3' target of human SAP130

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against SAP130 (SAP130 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330A_FokI-1x3

    Plasmid

    #63603

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330A_dCas9-1x5

    Plasmid

    #63599

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEPOR0SP0011

    Plasmid

    #117530

    Purpose

    Level0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-xCas9 3.7 (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458_SP5_2

    Plasmid

    #86363

    Purpose

    Encodes gRNA for 3' target of human SP5

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against SP5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. hIRF-1 gRNA #409 (Sa)

    Plasmid

    #98134

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    gRNA_hIRF1 promoter #409
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. hIRF-1 gRNA #351 (Sa)

    Plasmid

    #105283

    Purpose

    Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the Sa-Cas9 system

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Insert

    gRNA_hIRF1 promoter #351
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pX330A_dCas9-1x3

    Plasmid

    #63597

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCCM937

    Plasmid

    #58982

    Purpose

    Expresses avr-15 sgRNA in C.elegans.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    avr-15 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pCCM936

    Plasmid

    #58981

    Purpose

    Expresses avr-14 sgRNA in C.elegans.

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    avr-14 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pGY18

    Plasmid

    #221708

    Purpose

    Empty Cas9 vector for multiplex gene editing in fungi

    Depositor

    Kyle Pomraning

    Article

    Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

    Type

    Empty backbone

    Use

    CRISPR; Aspergillus

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRISPEY-Z3-Kan

    Plasmid

    #232102

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCRISPEY-Z3-Hyg

    Plasmid

    #232101

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMRS-AraC-dCas9

    Plasmid

    #220195

    Purpose

    mini-Tn7 dCas9 expression vector - AraC/pBAD promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    AraC/pBAD

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMRS-dCas9

    Plasmid

    #220193

    Purpose

    mini-Tn7 dCas9 expression vector

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    promoterless

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pDL25_Cas9-His Δcys E1207C

    Plasmid

    #206286

    Purpose

    Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    C80S, C574S, E1207C

    Promoter

    T7

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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