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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,137 results for: his

Showing: 3041 - 3080 of 22137 results

  1. pET-His6-Sumo-MBP-CfaN

    Plasmid
    #126016
    Purpose
    Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)
    Depositor
    Thomas Muir
    Insert
    His5-Sumo-MBP-CfaN
    Use
    Tags
    5xHis-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126016-plasmid-map-sequence-id-248770

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  5. Ana GvpC-SpyTag-His in pET28a

    Plasmid
    #85737
    Purpose
    Expresses His-tagged GvpC from Anabaena flos-aquae fused to SpyTag in E.coli
    Depositor
    Mikhail Shapiro
    Article
    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi
    Insert
    Gas vesicle protein C (fused to SpyTag and His-tagged)
    Use
    Tags
    His-tag and SpyTag
    Expression
    Bacterial
    Mutation
    Added an extra glycine after the methionine at th…
    Promoter
    T7
    Available Since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    85737-plasmid-map-sequence-id-173841

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  9. pCI_E01_mini-mAgrin-Myc-His-WPRE__LG1-3

    Plasmid
    #198141
    Purpose
    Express mini-Agrin in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    mini-Agrin (Agrn Mouse)
    Use
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available Since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    198141-plasmid-map-sequence-id-390123

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  10. pCI_E01_mini-mAgrin-Myc-His-WPRE__LG3

    Plasmid
    #198140
    Purpose
    Express mini-Agrin in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    mini-Agrin (Agrn Mouse)
    Use
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available Since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    198140-plasmid-map-sequence-id-390122

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  12. pET28a-ybbR-HIS-Xyl-DocI

    Plasmid
    #58711
    Purpose
    Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag
    Depositor
    Hermann Gaub
    Article
    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm
    Insert
    Xyl-DocI
    Use
    Tags
    HIS, HRV 3C, and ybbR
    Expression
    Bacterial
    Mutation
    changed Threonin 120 to Cysteine in Xylanase Doma…
    Promoter
    T7
    Available Since
    Aug. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58711-plasmid-map-sequence-id-90776

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  18. p6268 pDEST-59-His-Trx-HPV16E6

    Plasmid
    #24126
    Depositor
    Peter Howley
    Insert
    HPV16 E6 (E6, E6 HPV)
    Use
    Tags
    His and Trx
    Expression
    Bacterial and Mammalian
    Mutation
    none
    Promoter
    Available Since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  26. pET-His6-Sumo-IntNtr-eSrt2A

    Plasmid
    #126015
    Purpose
    Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)
    Depositor
    Thomas Muir
    Insert
    His6-Sumo-IntNtr-eSrt2A
    Use
    Tags
    6xHis-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    126015-plasmid-map-sequence-id-248768

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  30. AAP-COMP-blac-flag-his

    Plasmid
    #111001
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    aminopeptidase P (APP) (PF14_0517 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available Since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111001-plasmid-map-sequence-id-224546

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  33. pHybr_r2a>m2a(silent)-srt-his

    Plasmid
    #124807
    Purpose
    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.
    Depositor
    Ferenc Scheeren, Martijn Verdoes
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a (Fc silent)
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    L234A/L235A/N297A
    Promoter
    Available Since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124807-plasmid-map-sequence-id-251832

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  34. HOXA5 (human) HIS-tag pET

    Plasmid
    #8562
    Depositor
    Corey Largman
    Insert
    HOXA5 (HOXA5 Human)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    SacI site at amino acids 1-2 was used to clone in…
    Promoter
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  35. HOXC9 (murine) HIS-tag pET

    Plasmid
    #8559
    Depositor
    Corey Largman
    Insert
    HOXC9 (Hoxc9 Mouse)
    Use
    Tags
    His and T7
    Expression
    Bacterial
    Mutation
    makes protein by Western
    Promoter
    Available Since
    May 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  39. pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

    Plasmid
    #44072
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    6xGly linker, ADH1 transcription terminator, His3…
    Expression
    Mutation
    Promoter
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44072-plasmid-map-sequence-id-61692

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Showing: 3041 - 3080 of 22137 results