We narrowed to 16,849 results for: SHE

1. jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)

   Plasmid

   #79785

   Purpose

   genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a barcode landing pad

   Depositor

   Anthony Zador

   Insert

   MAPP-nL
   

   Use

   Synthetic Biology; Sindbis virus genomic plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pExp-GST

   Plasmid

   #112566

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-GST and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGM1192

   Plasmid

   #115683

   Purpose

   E. coli-Streptomyces shuttle vector for gene expression

   Depositor

   Günther Muth

   Insert

   mCherry, aac(3)IV, tsr
   

   Use

   Tags

   Strep-tagII on mCherry, codon optimized

   Expression

   Bacterial

   Mutation

   mCherry codon optimized for Streptomyces

   Promoter

   SF14*

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS2+ H2B-mTagBFP2

   Plasmid

   #99267

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   mTagBFP2-3XHA
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHN922

   Plasmid

   #178975

   Purpose

   Expression of GST-His8-GFP-nanobody

   Depositor

   Kazuhiro Abe

   Insert

   GST-His8-GFP-nanobody
   

   Use

   Affinity Reagent/ Antibody

   Tags

   GST, His8

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mPA-GFP-N1

   Plasmid

   #54712

   Purpose

   Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. mPA-GFP-C1

   Plasmid

   #54522

   Purpose

   Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pLenti-hSyn1-mScarlet/CAAX

   Plasmid

   #197592

   Purpose

   Lentiviral transfer vector for the neuronal expression of membrane-bound mScarlet (good visualization of neuronal processes)

   Depositor

   Patrik Verstreken

   Insert

   mScarlet-CAAX
   

   Use

   Lentiviral

   Tags

   CAAX

   Expression

   Mutation

   Promoter

   hSynI

   Available since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmSTARRseq1

   Plasmid

   #96945

   Purpose

   CpG free plasmid for testing effects of methylation on regulatory activity

   Depositor

   Jenny Tung

   Type

   Empty backbone

   Use

   Mstarr-seq screening vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CpG -free EF1 promoter

   Available since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZP06B

    Plasmid

    #232321

    Purpose

    Make sacB a counterselection marker to replace galK

    Depositor

    Chenggang Wu

    Insert

    PrpsJ-sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

    Plasmid

    #52421

    Purpose

    Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

    Depositor

    Bertrand Collet

    Insert

    Fusion NLS-EGFP-P2A-Cherry-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mu-GM-CSF-pCIneo

    Plasmid

    #163518

    Purpose

    Expresses murine GM-CSF in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine GM-CSF
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEC1.2/EC1.1_spCas9_GFP

    Plasmid

    #161933

    Purpose

    CRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selection

    Depositor

    Christian Fankhauser

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid

    #72299

    Purpose

    Express gene of interest under CB promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin + CMV enhancer

    Available since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. AAV-hSYN-TdTomato

    Plasmid

    #193019

    Purpose

    AAV vector plasmid expressing TdTomato under the human synapsin (hSYN) promoter

    Depositor

    Bart Nieuwenhuis

    Insert

    TdTomato
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MZB174

    Plasmid

    #228487

    Purpose

    10xHis-SUMO (Smt3) fused, HRV-3C cleavable, GST-tag for rapid cloning/DNA assembly of pulldown targets via NotI restriction site for E. coli based protein expression

    Depositor

    Richard Baker

    Type

    Empty backbone

    Use

    Tags

    10xHis-SUMO(Smt3)-GST-HRV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAdx-CMV-iCre-P2A-tdTomato

    Plasmid

    #73351

    Purpose

    Express iCre and tdTomato under CMV promoter

    Depositor

    Kazuhiro Oka

    Insert

    CMV-iCre-tdtomato
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEIAV-SIN6.1 CBGFPW

    Plasmid

    #44173

    Depositor

    John Olsen

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    chromophore mutations

    Promoter

    CB, CMV enhancer/chicken beta-actin promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SUMO-PNGaseF

    Plasmid

    #228498

    Purpose

    10xHis-SUMO (Smt3) tagged PNGaseF (E. coli based protein expression)

    Depositor

    Richard Baker

    Insert

    ngl
    

    Use

    Tags

    10xHis-SUMO(Smt3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SV40-eGFP-Z1

    Plasmid

    #127638

    Purpose

    Minimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistance

    Depositor

    Jordan Green

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.3_SARS2_JN.1

    Plasmid

    #218678

    Purpose

    expressing SARS-CoV-2 JN.1 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Insert

    Spike (JN.1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L455S on the basis of BA.2.86

    Promoter

    CMV

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMK33-GW-TurboID-V5

    Plasmid

    #196281

    Purpose

    Gateway destination vector to express C-terminal TurboID fusion under metallothionein promoter, for making stable cell lines

    Depositor

    Norbert Perrimon

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-Syn-Flex-TREx2-tTA

    Plasmid

    #191210

    Purpose

    Cre dependent TRE-tTA expression under the control of Syn promoter

    Depositor

    Janelia Viral Tools

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDR125

    Plasmid

    #37150

    Depositor

    Dale Ramsden

    Insert

    lig4 (LIG4 Human)
    

    Use

    Tags

    H6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

25. SPLICSFAST-ER-PO-P2A

    Plasmid

    #214894

    Purpose

    Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-PO-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX2-Cas9

    Plasmid

    #85811

    Purpose

    Arabinose inducible Cas9 in a broad host range backbone.

    Depositor

    Ryan Gill

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CaMKII-GFP

    Plasmid

    #64545

    Purpose

    AAV-mediated expression of GFP under the CaMKII promoter.

    Depositor

    Edward Boyden

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Nov. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. R6K_tac_GFPmut3_ChInt

    Plasmid

    #187381

    Purpose

    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible GFPmut3

    Depositor

    Roi Avraham

    Insert

    GFPmut3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLentiGuide-BlastR

    Plasmid

    #196970

    Purpose

    Lentiviral acceptor vector for pegRNAs with blasticidin resistance

    Depositor

    Leopold Parts

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA4_ChR2opt_mKATE

    Plasmid

    #67958

    Purpose

    optical depolarization of mammalian cells

    Depositor

    Andreas Offenhäusser

    Insert

    Channelrhodopsin 2 opt
    

    Use

    Tags

    mKatushka

    Expression

    Mammalian

    Mutation

    synthetically produced for mammalian optimized co…

    Promoter

    CMV -TetON

    Available since

    Sept. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. K44A dynamin 2 pEGFP

    Plasmid

    #34687

    Depositor

    Sandra Schmid

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-hSyn-GRAB_NTS1.0

    Plasmid

    #180006

    Purpose

    Expresses the neurotensin sensor GRAB_NTS1.0 in neurons

    Depositor

    Yulong Li

    Insert

    neurotensin sensor GRAB_NTS1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1 ShhN

    Plasmid

    #37680

    Depositor

    Philip Beachy

    Insert

    shh (Shh Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    soluble form (see comments)

    Promoter

    CMV

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET-21a(+)/MBP-His

    Plasmid

    #38006

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    MBP (Maltose binding protein)
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

35. pLinker-2xTy-HXGPRT-LoxP

    Plasmid

    #86664

    Purpose

    CRISPR tagging with 2xTy in Toxoplamsa gondii

    Depositor

    David Sibley

    Insert

    Linker-2xTy-HXGPRT 3'UTR
    

    Use

    Expression in toxoplasma gondii

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MSCV-Six1

    Plasmid

    #49263

    Purpose

    retroviral expression of human Six1 and GFP

    Depositor

    Heide Ford

    Insert

    Six1 (SIX1 Human)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGLO-Magenta-AZA

    Plasmid

    #220784

    Purpose

    Expresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    magenta chromoprotein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJA5 H-2K(b)

    Plasmid

    #180441

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    H-2K(b)
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. SPLICSFAST-LY-MT-P2A

    Plasmid

    #214897

    Purpose

    Expresses equimolar Lysosome- and Mitochondria- targeted split-FAST fragments for Short-range LY-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-LY-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCS2+ H2B-Katushka2

    Plasmid

    #99266

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein Katushka2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Insert

    Katushka2-3XHA
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. SPLICSFAST-PO-MT-P2A

    Plasmid

    #214899

    Purpose

    Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-PO-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pYZ205

    Plasmid

    #104586

    Purpose

    Express SNAP-6His-CBD

    Depositor

    Yinhua Zhang, New England Biolabs

    Insert

    SNAP-6His-CBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

43. pLenti-hSyn1-mClover3/CAAX

    Plasmid

    #197591

    Purpose

    Lentiviral transfer vector for the neuronal expression of membrane-bound mClover3 (good visualization of neuronal processes)

    Depositor

    Patrik Verstreken

    Insert

    mClover3-CAAX
    

    Use

    Lentiviral

    Tags

    CAAX

    Expression

    Mutation

    Promoter

    hSynI

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSRKBB-empty

    Plasmid

    #59449

    Purpose

    Contains BioBrick prefixed and suffixed insert with a modified lac promoter, Shine-Delgarno sequence, 5’ & 3’ MCS, 3’ 6xHis tag.

    Depositor

    Claudia Schmidt-dannert

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    modified lac

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. RHPMP192 (pLifeAct sfGFP)

    Plasmid

    #88843

    Purpose

    Localization by Fluorescence microscopy of lifeAct fused to super folder GFP

    Depositor

    Ricardo Henriques

    Insert

    LifeAct
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. EcMscL

    Plasmid

    #92418

    Purpose

    E. coli Mechanosensitive Channel of Large Conductance

    Depositor

    Douglas Rees

    Insert

    MscL (mscL E. coli)
    

    Use

    Tags

    6x Histidine Tag and Thrombin Cleavage Site betwe…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. AAV-U6-gRNA-TnT-Cre

    Plasmid

    #132551

    Purpose

    For loss-of-function experiments in cardiomyocytes

    Depositor

    William Pu

    Inserts

    TnT-Cre
    U6-gRNA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TnT and U6

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCS2+ H2B-TagRFP-T

    Plasmid

    #99271

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein TagRFP-T in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Insert

    TagRFP-T
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. SPLICSFAST-NU-MT-P2A

    Plasmid

    #214898

    Purpose

    Expresses equimolar Nucleus- and Mitochondria- targeted split-FAST fragments for Short-range NU-MT Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-NU-MT-P2A
    

    Use

    Tags

    none

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMZ379

    Plasmid

    #74215

    Purpose

    Combined sgRNA/Cas9 vector with NatMX marker

    Depositor

    Mikel Zaratiegui

    Inserts

    Cas9
    rrk1:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Silent muation of the CspCI site

    Promoter

    adh1 and rrk1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge