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pEFmEGFP-mDia2

Plasmid

#25407

Depositor

Arthur Alberts

Article

Tominaga et al Mol Cell. 2000 Jan . 5(1):13-25.

Insert

mDia2 (Diaph3 Mouse)

Tags

EGFP, His, and Myc

Expression

Mammalian

Mutation

Encodes mDia2

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +1

Plasmid

#66940

Purpose

CRISPaint frame selector +1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +1

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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AAV.TBG.PI.Cre.rBG

Plasmid

#107787

Purpose

AAV vector expressing Cre from TBG promoter

Depositor

James M. Wilson

Has Service

AAV8

Insert

Cre

Use

AAV

Expression

Mammalian

Promoter

TBG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE GFP

Plasmid

#10668

Purpose

Empty backbone for retroviral gene expression. Select recipient cells using GFP.

Depositor

William Hahn

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-pBAD

Plasmid

#54572

Purpose

Localization: Protein Expression Vector , Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis, T7, and mTagBFP2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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hCas9

Plasmid

#41815

Purpose

Expresses human codon optimized Cas9 nuclease for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pAAVS1-tet-iCas9-BFP2

Plasmid

#125519

Purpose

all-in-one-inducible Cas9

Depositor

Jason Moffat

Article

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Insert

AAVS1-TET-ON 3G-SpCas9-P2A-BFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO1-shYAP1

Plasmid

#27368

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

shYAP1

Expression

Mammalian

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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EF.GFP

Plasmid

#17616

Depositor

Linzhao Cheng

Article

Cui et al Blood. 2002 Jan 15. 99(2):399-408.

Insert

EF-1a promoter (EEF1A1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pINDUCER11 (miR-RUG)

Plasmid

#44363

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-cdk5GFP

Plasmid

#1346

Depositor

Li-Huei Tsai

Insert

CDK5 (CDK5 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-STAT3

Plasmid

#111934

Purpose

Expresses human STAT3 fused to GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

Insert

Signal transducer and activator of transcription 3 (STAT3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T MDM2 WT

Plasmid

#16237

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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SepOTSλ

Plasmid

#68292

Purpose

Phosphoserine orthogonal translation system (preferred variant)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 4xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-SARS2-B.1.617.2

Plasmid

#172320

Purpose

expressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.2 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

T19R, 156G, 157-158del, L452R, T478K, D614G, P681…

Promoter

CMV-F

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS2-Spike

Plasmid

#145032

Purpose

Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV-2 Spike (S Synthetic)

Tags

C9

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-Ataxin3Q84

Plasmid

#22123

Depositor

Henry Paulson

Article

Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

Insert

Ataxin 3 (ATXN3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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3XFlagNICD1

Plasmid

#20183

Depositor

Raphael Kopan

Article

Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

Insert

Notch 1 Intracellular Domain (Notch1 Mouse)

Tags

3XFLAG

Expression

Mammalian

Mutation

Contains murine Notch 1 Intracellular Domain (Val…

Promoter

CMV

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mRuby2

Plasmid

#40260

Purpose

RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

Depositor

Michael Lin

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Insert

mRuby2

Tags

His6 and Xpress

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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