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4,202 results

Showing: 1541 - 1560 of 4202 results

  2. pDD162 (Peft-3::Cas9 + Empty sgRNA)

    Plasmid
    #47549
    Purpose
    Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    Empty sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47549-plasmid-map-sequence-id-228099

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  11. AAV.TBG.PI.Cre.rBG

    Plasmid
    #107787
    Purpose
    AAV vector expressing Cre from TBG promoter
    Depositor
    James M. Wilson
    Has Service
    AAV8
    Insert
    Cre
    Use
    AAV
    Expression
    Mammalian
    Promoter
    TBG
    Available Since
    May 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    107787-plasmid-map-sequence-id-259375

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  13. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid
    #92099
    Purpose
    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Expression
    Mammalian
    Promoter
    Tet-On 3G Bidirectional
    Available Since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92099-plasmid-map-sequence-id-180428

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  14. pcDNA-RLuc8

    Plasmid
    #87121
    Purpose
    Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.
    Depositor
    Sanjiv Sam Gambhir
    Article
    Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-
    Insert
    Renilla Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…
    Promoter
    CMV
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87121-plasmid-map-sequence-id-175252

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  17. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Expression
    Insect
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112645-plasmid-map-sequence-id-217990

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  18. ERM-APEX2

    Plasmid
    #79055
    Purpose
    Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    APEX2 anchored to the cytosolic face of the ER membrane
    Use
    Lentiviral
    Tags
    Residues 1-27 of P450 oxidase 2C1 and V5
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    79055-plasmid-map-sequence-id-419573

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Showing: 1541 - 1560 of 4202 results