162,619 results

1. pNDGG054

   Plasmid

   #231323

   Purpose

   BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmR

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEDA5_GFPmut3_Y66H

   Plasmid

   #80085

   Purpose

   Positive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.

   Depositor

   Timothy Whitehead

   Article

   Wrenbeck et al Nat Methods. 2016 Nov;13(11):928-930. do

   Insert

   GFPmut3_Y66H
   

   Use

   Tags

   6x-His tag

   Expression

   Bacterial

   Mutation

   changed Tyrosine 66 to Histidine

   Promoter

   proB

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBR_attB(bxb)_ccdB_lox_v2

   Plasmid

   #198040

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-PDE4D2cat-ICUE4

   Plasmid

   #181847

   Purpose

   ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
   

   Use

   Tags

   ECFP, PDE4D2(86-418), and cpVenus(L194)

   Expression

   Mammalian

   Mutation

   Glutamine 122 in Epac1 domain mutated to glutamat…

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX305_3xFLAG_STAT1

   Plasmid

   #220323

   Purpose

   Expresses full length human STAT1 with an N-terminal 3xFLAG tag

   Depositor

   Jonathan Brown

   Insert

   STAT1 alpha (STAT1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   PGK

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MIB2-FLAG

   Plasmid

   #37117

   Depositor

   Martin Dorf

   Article

   Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

   Insert

   MIB2, mindbomb E3 ubiquitin protein ligase 2 (MIB2 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pLenti-SF3B1-K700E-reporter

   Plasmid

   #200318

   Purpose

   A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

   Depositor

   Lili Wang

   Article

   Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

   Insert

   BSD-2A-EGFPi
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-ClpX

   Plasmid

   #234733

   Purpose

   Human-codon optimized E. coli ClpX in mammalian cells

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ClpX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCPP5372

   Plasmid

   #128733

   Purpose

   Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

   Depositor

   Alan Collmer

   Article

   Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNCS-mCyRFP1

    Plasmid

    #84545

    Purpose

    Bacterial expression of mCyRFP1

    Depositor

    Michael Lin

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCyRFP1
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. LT3GEP

    Plasmid

    #111172

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    T3G

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDH

    Plasmid

    #133129

    Purpose

    A recombinant plasmid expressing mdh (malate dehydrogenase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    malate dehydrogenase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    malate dehydrogenase promoter

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNANOG-Luc

    Plasmid

    #25900

    Depositor

    Ren-he Xu

    Article

    Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

    Insert

    NANOG promoter (NANOG Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUCM-CLYBL-hNIL

    Plasmid

    #105841

    Purpose

    Donor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuron

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    mCherry
    rtTA3G
    

    Use

    CRISPR and TALEN

    Tags

    Expression

    Mutation

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. ARR3tk-eGFP/SV40-mCherry

    Plasmid

    #132360

    Purpose

    To measure transcriptional activity of Androgen Receptor

    Depositor

    Charles Sawyers

    Article

    Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

    Insert

    AR responsive elements (AR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUb PspCas13b + PspCas13b guide RNA

    Plasmid

    #176305

    Purpose

    Expresses PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Use

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E113K, N265S- please depositor comments

    Promoter

    Ubi-p63e

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW-SPI1

    Plasmid

    #162824

    Purpose

    Doxycycline-inducible overexpression of human SPI1

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB18cmBFPAzurite

    Plasmid

    #14034

    Depositor

    Patrick Daugherty

    Article

    Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

    Insert

    Azurite
    

    Use

    Yeast/bacteria optimized codons

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-3xHA-Puro

    Plasmid

    #207729

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

    Plasmid

    #67976

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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