We narrowed to 5,256 results for: pcdna3.1

1. GFPxm163

   Plasmid

   #193480

   Purpose

   Turn-off fluorescent sensor for chloride

   Depositor

   Sheel Dodani

   Article

   Peng et al Chem Sci. 2022 Oct 6;13(43):12659-12672.

   Insert

   GFPxm163
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ZipGFP1-10_TEV

   Plasmid

   #81242

   Purpose

   Zipped GFP1-10 with TEV cleavage sequence T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

   Insert

   zipped GFP11 with TEV cleavage sequence T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRPML1-CFP

   Plasmid

   #18827

   Depositor

   Craig Montell

   Article

   Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

   Insert

   TRPML1 (MCOLN1 Human)
   

   Use

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2008

   Availability

   Academic Institutions and Nonprofits only

4. mito-mGarnet

   Plasmid

   #104309

   Purpose

   codes for a fusion of fluorescent protein mGarnet and mito, stains mictochondrial content in live cells, i.e. human cytochrome c oxidase subunit VIII, the mitochondrial targeting sequence mito

   Depositor

   Ulrich Nienhaus

   Article

   Hense et al Sci Rep. 2015 Dec 9;5:18006. doi: 10.103

   Insert

   mito-mGarnet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. rASAP

   Plasmid

   #178205

   Purpose

   Ratiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.

   Depositor

   Stefan Heinemann

   Article

   Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

   Insert

   rASAP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 4xHRE_minCMV-sfGFP-CMV_dsRed Exp

   Plasmid

   #117401

   Purpose

   sfGFP expressed from hypoxia-inducible minimal CMV promoter and dsRed expressed from consitutive CMV promoter

   Depositor

   Yvonne Chen

   Article

   Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

   Insert

   SuperFolder GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Mammalian_SpCas9_Target-AID2S

   Plasmid

   #188648

   Purpose

   Expresses SpCas9_Target-AID2S in mammalian cells

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Use

   Tags

   UGI

   Expression

   Mammalian

   Mutation

   D10A for SpCas9

   Promoter

   pCMV

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTS1017-Tier1-OTetO7-PCMVmin-SEAP

   Plasmid

   #169586

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP (OTetO7-PCMVmin-SEAP-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO7-PCMVmin

   Available since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAL175

   Plasmid

   #22276

   Purpose

   Expresses YFP-PIF for red-light-induced binding to Phy

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   mYFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. DHFR-myc

    Plasmid

    #20214

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    DHFR (folA E. coli)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Mammalian_SpCas9_Target-AID3S

    Plasmid

    #188649

    Purpose

    Expresses SpCas9_Target-AID3S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID3S-UGI
    

    Use

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3 Rod Opsin

    Plasmid

    #109361

    Purpose

    Human rod opsin

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Rod opsin (RHO Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-GATA3

    Plasmid

    #1332

    Depositor

    Gokhan Hotamisligil

    Article

    Tong et al Science 2000 Oct 6;290(5489):134-8.

    Insert

    GATA3 (Gata3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YFP Gamma 9

    Plasmid

    #36107

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 9
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    HIs tag between YFP and insert

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

15. ER2-mhYFP

    Plasmid

    #186528

    Purpose

    Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    Monomeric hyperfolder YFP
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    hfYFP-S147P/L195M/V206K

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNIR-Fbactin

    Plasmid

    #184677

    Purpose

    NIR-Fb to actin

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fbactin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-Mirk (pHX9F)

    Plasmid

    #17846

    Depositor

    Eileen Friedman

    Article

    Lee et al Cancer Res. 2000 Jul 1. 60(13):3631-7.

    Insert

    Mirk (DYRK1B Human)
    

    Use

    Tags

    6xHis and Anti-XPress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

18. miniCMV-iRFP670-tDeg

    Plasmid

    #185403

    Purpose

    iRFP670-tDeg fluorogenic protein

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    iRFP670-tDeg
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    miniCMV (GGTAGGCGTGTACGGTGGGAGGCCTATATAAGCAGAGCT)

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZ149-pcDNA3-BhCas12b_v4

    Plasmid

    #122446

    Purpose

    Expresses BhCas12b_v4 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    CMV-NLS-BhCas12b_v4-NLS-3xHA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOVC1_TtCarH-CBD_X

    Plasmid

    #131179

    Purpose

    Expresses TtCarH-CBD (Thermus termophilus) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    TtCarH-CBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOVC1_MxCarH-CBD_X

    Plasmid

    #131178

    Purpose

    Expresses MxCarH-CBD (Myxococcus xanthus) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    MxCarH-CBD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EGFP-Tubulin.K40Q

    Plasmid

    #32912

    Depositor

    Tso-Pang Yao

    Article

    Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

    Insert

    Tubulin (TUBA1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K40Q

    Promoter

    CMV

    Available since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

23. rArc

    Plasmid

    #178206

    Purpose

    Ratiometric version of the genetically encoded voltage indicator ArcLight-Q239.

    Depositor

    Stefan Heinemann

    Article

    Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

    Insert

    rArc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Calreticulin-SuperNova-KDEL

    Plasmid

    #131857

    Purpose

    Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Use

    Tags

    KDEL ER-retention signal and Signal sequence of h…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mNeonGreen-IETD-NanoLuc

    Plasmid

    #98288

    Purpose

    caspase-8 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-ER-ZapCY1

    Plasmid

    #36321

    Depositor

    Amy Palmer

    Article

    Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Insert

    ER-ZapCY1 high affinity Zn(II) sensor
    

    Use

    Tags

    KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-LBR-EGFP-polyA

    Plasmid

    #59748

    Purpose

    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pZ148-BhCas12b-sgRNA-scaffold

    Plasmid

    #122448

    Purpose

    Expresses sgRNA for BhCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BhCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3 Plk4(Sak) D154A (Nigg HR26)

    Plasmid

    #41164

    Depositor

    Erich Nigg

    Article

    Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

    Insert

    Plk4 D154A (PLK4 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    D154A

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. VinculinTS-A50I/I997A

    Plasmid

    #111829

    Purpose

    Vinculin tension sensor with point mutations affecting ability to bind talin and actin

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (I997A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed vinculin alanine 50 to isoleucine and iso…

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMM310-Myc

    Plasmid

    #80053

    Purpose

    Expression of a Myc tagged version of the BlaM-Vpr fusion protein

    Depositor

    Wesley Sundquist

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    Myc-BlaM-Vpr
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    N-terminal Myc tag added to construct pMM310

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV_mCherry_2A_HA_2xMS2-VP64

    Plasmid

    #68419

    Purpose

    Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    MS2
    

    Use

    Synthetic Biology

    Tags

    HA and VP64

    Expression

    Mammalian

    Mutation

    MS2: V75E/A81G

    Promoter

    CMV

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Cenp-B INCENP GFP

    Plasmid

    #45238

    Purpose

    Target INCENP to centromeres via full length CENP-B

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B, INCENP-Δcen, GFP
    

    Use

    Tags

    GFP and full length CENP-B

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pVH231-Tier1-PmPGK1-mTagBFP2

    Plasmid

    #169520

    Purpose

    Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric Tag blue fluorescent protein 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA-LBR-mRFP1-polyA

    Plasmid

    #59749

    Purpose

    Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    LBR-mRFP1
    

    Use

    Tags

    mRFP1

    Expression

    Mammalian

    Mutation

    aa 1-249 of LBR

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA-Golgi-ZapCY1

    Plasmid

    #36322

    Depositor

    Amy Palmer

    Article

    Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Insert

    Golgi-ZapCY1 high affinity Zn(II) sensor
    

    Use

    Tags

    GalT (first 60 amino acids)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCD/NL-BH*deltavpu/RT-

    Plasmid

    #136985

    Purpose

    Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity

    Depositor

    Jakob Reiser

    Article

    Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

    Insert

    HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    carries D110E mutation in the reverse transcripta…

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Fra-1

    Plasmid

    #187906

    Purpose

    Expresses Fra-1 in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

    Insert

    Fra1 (Fosl1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVH233-Tier1-PmPGK1-eYFP

    Plasmid

    #169522

    Purpose

    Tier-1 vector encoding PmPGK1-driven eYFP expression (PmPGK1-eYFP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. FRB-myc

    Plasmid

    #20228

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only