We narrowed to 13,473 results for: Mpl

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eSpCas9(1.1)_No_FLAG

Plasmid

#79877

Purpose

FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P22

Plasmid

#120033

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PROM5_MpEF1a

Plasmid

#136113

Purpose

Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM5_MpEF1a Mapoly0024s0116

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5 Flag-NFRKB

Plasmid

#19789

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

NFRKB (NFRKB Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

V1200A

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax(M47)

Plasmid

#23286

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

Tax(M47)

Expression

Mammalian

Mutation

L319R L320S

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-hAtg14

Plasmid

#24295

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 27, 2010

Availability

Academic Institutions and Nonprofits only

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H6hSDHA

Plasmid

#113045

Purpose

Hetero expression of His-tagged human SDHA

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

H6hSDHA (SDHA Human)

Tags

His6 (MGSSHHHHHHSQDP-)

Expression

Bacterial

Mutation

Insert begins at amino acid 43

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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1307 pcDNA3 GFP PTEN A4

Plasmid

#10760

Depositor

William Sellers

Article

Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Mutation

A4: S380A, T382A, T383A, S385A

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pMaCTag-P26

Plasmid

#120037

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-BMAL1

Plasmid

#186824

Purpose

Expresses BMAL1 in mammalian cells

Depositor

Hung-Chun Chang

Article

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

bmal1 (Bmal1 Mouse)

Expression

Mammalian

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1 WT IRES GFP

Plasmid

#133816

Purpose

Encodes full-length wild type synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Promoter

hSyn (human synapsin I promoter)

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS gag-gfp/deltavpx

Plasmid

#115807

Purpose

SFFV promoter expresses gag-gfp fusion with no ORF after CypA promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gag-gfp

Use

Lentiviral

Promoter

SFFV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRRP12_K

Plasmid

#146766

Purpose

Mammalian Expression of HsRRP12

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsRRP12 (RRP12 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 deltaKH

Plasmid

#17688

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

aa 157-256 deleted (KH domain)

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA-LBR-mRFP1-polyA

Plasmid

#59749

Purpose

Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

LBR-mRFP1

Tags

mRFP1

Expression

Mammalian

Mutation

aa 1-249 of LBR

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CcVA

Plasmid

#24588

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pSplit-CO-hDib1

Plasmid

#58788

Purpose

Express LucC-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLBM0010

Plasmid

#172816

Purpose

Level 2 module containing HSP21-GFP

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

[35S-P19][35S-CTPSSU-mHSP21-GFP]

Use

Synthetic Biology

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_BIRC6

Plasmid

#197974

Purpose

Entry clone for wt BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Use

Other

Mutation

L1332V

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTP924 pHAGE2 CMV GFP

Plasmid

#199392

Purpose

Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLX-UBC_NKX2-1_Short_V5-GFP

Plasmid

#221499

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

UBC

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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PX429 SpCas9 N863A nickase

Plasmid

#68706

Purpose

SpCas9 N863A nickase

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

SpCas9 N863A nicking mutant

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pISFba1

Plasmid

#226820

Purpose

Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISFba1

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pISFba1reRNA

Plasmid

#226822

Purpose

Expressing ISFba1 guide RNA targeting maeB site controled by PJ23119

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISFba1 none target guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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LAMP1-ABKAR

Plasmid

#65068

Purpose

Expression of ABKAR biosensor targeted to the lysozome

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

ABKAR biosensor

Tags

Lamp1

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only
Nuc-ABKAR

Plasmid

#61511

Purpose

Expression of nuclear AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

NLS (see comments)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
modRNAc0-Cas9

Plasmid

#170180

Purpose

IVT template plasmid for making modified Cas9 RNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

Cas9

Use

Ivt template plasmid

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P33

Plasmid

#120018

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with FLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

FLAG

Use

Pcr template

Tags

FLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo VPS51-13Myc

Plasmid

#175094

Purpose

13Myc tagged VPS51 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Gershlick et al Hum Mol Genet. 2019 May 1;28(9):1548-156

Insert

VPS51 (VPS51 Human)

Tags

13Myc

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-TAX1BP1(ΔUBD)

Plasmid

#119689

Purpose

For mammalian expression of TAX1BP1 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

UBD deletion (1-755)

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-5X1 Flag RbBP5

Plasmid

#15551

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

RbBP5 (RBBP5 Human)

Tags

Flag and GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pSPlit-OC-hDib1

Plasmid

#58789

Purpose

Express hDib1-LucC fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDib1 (TXNL4A Human)

Tags

Flag and Luciferase (394-550aa)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z34

Plasmid

#120062

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXWJ109Hg-gfp

Plasmid

#123148

Purpose

A mercury sensor with optimized intracellular receptor density. pSB3K3 carrying J109-32merR-t-PmerT-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-Z22

Plasmid

#120065

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE6

Plasmid

#79476

Purpose

sgRNA shuttle vector; module 1E

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

pAtU6-ccdB-sgRNA (ccdB(letD) )

Use

CRISPR and Synthetic Biology

Mutation

BsaI site eliminated

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Integrin beta1(1-455)-tev-BHCys

Plasmid

#27315

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin beta1 (ITGB1 Human)

Expression

Mammalian

Mutation

beta1(1-455)+GG+TEV+GGKN+BHCys+TG+His6

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only
pET28b::mAID

Plasmid

#15923

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

AID (Aicda Mouse)

Tags

His

Expression

Bacterial

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Golgi-ABKAR

Plasmid

#61507

Purpose

Expression of AMPK biosensor targeted to the Golgi

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

Golgi-targeting signal (see comments)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
CCR5-donor-MCS

Plasmid

#192269

Purpose

Donor template for CRISPR Genome Editing

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

MCS within donor template

Use

CRISPR

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
JAF2.13

Plasmid

#45608

Depositor

Gregory Pazour

Article

Follit et al Mol Biol Cell. 2006 Sep . 17(9):3781-92.

Insert

IFT20 (Ift20 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pDF0386 pcmv-hudiscas7-11-s1006-gggs-d1221

Plasmid

#186983

Purpose

mammalian expression of cas7-11-s1006-gggs-d1221

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122

Expression

Mammalian

Mutation

s1006-gggs-d1221 deletion

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE5400_pMBP_CDS dropout vector

Plasmid

#221189

Purpose

Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Tags

10x His-tag + E. coli maltose binding protein (MB…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCsC_spacer

Plasmid

#136077

Purpose

Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCsB

Plasmid

#136068

Purpose

Acceptor plasmid for even level position 2, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCsC

Plasmid

#136069

Purpose

Acceptor plasmid for even level position 3, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCsE

Plasmid

#136070

Purpose

Acceptor plasmid for even level position 4, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
PXWABS62(E11-TEV)

Plasmid

#123157

Purpose

For TEV-based leakiness regulation. pSB4A3 carrying ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

ParsR-ABS62-32ECF11-t-Pecf11-32malE-te-TEV-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

ParsR-ABS62, Pecf11

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 13,473 results for: Mpl