2,202 results

Showing: 281 - 320 of 2202 results

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pCS2-BiNLS-fYap

Plasmid

#176623

Purpose

expresses optofYap in zebrafish

Depositor

Timothy Saunders

Article

Toh et al EMBO Rep. 2022 Sep 5;23(9):e54401. doi:

Insert

Yes associated protein (yap1 Zebrafish)

Use

Zebrafish expression

Tags

mCherry

Promoter

SP6

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSYC-97

Plasmid

#31565

Depositor

Seok-Yong Choi

Article

Kim et al PLoS One. 2011 . 6(4):e18556.

Insert

pCS4+-NLS-EGFP-P2A-mCherry-CAAX

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-TagBFP

Plasmid

#117777

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pT2KXIG (Tol2)-tp63:ikk1KD:GFP

Plasmid

#105645

Purpose

Kinase-deleted Ikk1(alpha) expression in basal keratinocytes

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

tp63:ikk1KD:GFP (chuk Zebrafish)

Tags

GFP

Expression

Mammalian

Mutation

p63 promoter + ikk1 (kinase domain deleted) + GFP

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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p3E_he1a:ECFP

Plasmid

#113880

Purpose

p3E entry vector for Three Multisite Gateway

Depositor

Marnie Halpern

Insert

he1a (he1.1 Zebrafish)

Use

Zebrafish danio expression

Tags

ECFP

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rac1

Plasmid

#109529

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Zebrafish ArcLight Q175

Plasmid

#53616

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Zebrafish ArcLight-Q175 (tpte Synthetic, Aequorea victoria, Zebrafish)

Expression

Mammalian

Mutation

Dr-VSD contains R153Q mutation and an amino acid …

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin4a-Clover

Plasmid

#163853

Purpose

Zebrafish muscle specific expression of Cavin4a-Clover, Parton lab clone DPV

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4a (cavin4a Zebrafish)

Use

Zebrafish expression

Tags

Clover

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTol2_LSEC:Cas9green; erbb2_gRNA171

Plasmid

#199338

Purpose

pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

LSEC
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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Zed_vector_tol2_-2.1prox1a_1:EGFP_XCA:DsRed2

Plasmid

#218207

Purpose

Zebrafish -2.1prox1a_1 (mouse conserved) enhancer reporter in the lymphatic valve from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-2.1prox1a_1

Use

Zebrafish expression

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rac1-DN

Plasmid

#109530

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p155ME-loxP-dsR-loxP-Cas9-t2A-GFP

Plasmid

#227774

Purpose

Middle-entry vector with dsRed flanked by loxP sites and spCas9-GFP for Gateway cloning

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

loxP-dsR-loxP

Use

CRISPR

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Phat2-dr-cPla2-C2-EGFP-6H

Plasmid

#187001

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Tol2-cmlc2-Trpa1b-2A-EGFP

Plasmid

#106405

Purpose

For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

Depositor

Randall Peterson

Article

Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

Inserts

cmlc2 promoter
Trpa1b (trpa1b Zebrafish)

Use

Gateway Cloning

Tags

2A-EGFP

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCK052 p5E exorh:mCherry

Plasmid

#195953

Purpose

Gateway p5E vector with reporter

Depositor

Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

exorh:mCherry

Use

Other

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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M58 pPET28b-TEV-full-length zebrafish beta catenin

Plasmid

#17199

Depositor

Randall Moon

Insert

beta catenin (ctnnb1 Zebrafish)

Tags

HIS

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
MiniCoopR mitfa:KIT L576P

Plasmid

#118848

Purpose

Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

KIT L576P (KIT Human, Zebrafish)

Use

Zebrafish expression, tol4

Mutation

KIT L576P

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -0

Plasmid

#180010

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-acta1:QFGal4-SV40pA

Plasmid

#155121

Purpose

Tol2 vector for skeletal-muscle expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

acta1:QFGal4

Use

Zebrafish expression

Promoter

acta1

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-Cavin4b-TurboID

Plasmid

#163851

Purpose

In vitro transcription of Cavin4b-TurboID. Parton lab clone JTA

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4b (cavin4b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pT3TS-CD44b-TurboID

Plasmid

#163850

Purpose

In vitro transcription of CD44b-TurboID. Parton lab clone JTJ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

CD44b (cd44b Zebrafish)

Use

In vitro transcription of rna

Tags

TurboID

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pPRISM-gal4-VP16-gcry-eGFP

Plasmid

#117779

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-U6a-polg guide

Plasmid

#107598

Purpose

An entry vector with U6a promoter driving polg guide RNA expression

Depositor

Qing Deng

Article

Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

Insert

polg guide RNA sequence

Use

CRISPR

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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mpeg:lamp2-mCherry-stop; cmlc2:GFP

Plasmid

#213740

Purpose

Expresses Lamp2-mCherry in the macrophage lineage of zebrafish with a green heart transgenesis marker

Depositor

William Talbot

Article

Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

Insert

lamp2-mCherry

Use

Zebrafish

Promoter

mpeg1.1

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

Plasmid

#118842

Purpose

Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

cdkn2a gRNA (LOC100329528 Zebrafish)

Use

CRISPR; Tol2

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk18

Plasmid

#209000

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk18 (kcnk18 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ZE31 Prickle-1-pCS2P+

Plasmid

#17072

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pGTag-eGFP-CAAX-SV40

Plasmid

#117815

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E Lck

Plasmid

#135201

Purpose

T-cell marker

Depositor

David Tobin

Insert

Lck promoter

Use

Synthetic Biology

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag-eGFP-B-actin

Plasmid

#117814

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Zebrafish aBb crystallin 2kb promoter/AcGFP

Plasmid

#98099

Purpose

Zebrafish aBb crystallin 2kb promoter segment driving GFP expression

Depositor

Mason Posner

Article

Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

Insert

Alpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)

Use

Gfp expressing

Tags

GFP

Promoter

Danio alpha Bb-crystallin 2 kb fragment (-2092/-1)

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2-actinb-kcna1a-IRES-EGFP

Plasmid

#164965

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcna1a (kcna1a Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPRISM-Stop-gcry-mRFP

Plasmid

#117772

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

Plasmid

#180011

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Phat2-dr-cPla2-C2-Y90F-V91F-EGFP-6H

Plasmid

#187186

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Mutation

Y90F, V91F

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-Rab13 in pCSDEST2

Plasmid

#140879

Purpose

Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab13 (rab13 Zebrafish)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGTag-TagRFP-B-actin

Plasmid

#117808

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

Plasmid

#172535

Purpose

Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

-3.5ubb:plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F and R415H mutations obse…

Promoter

zebrafish ubb

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab7a

Plasmid

#109723

Purpose

Multisite gateway vector for 3' tagging with rab7a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7a (rab7a Zebrafish)

Use

Zebrafish plasmids

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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2,202 results