We narrowed to 5,784 results for: pcas

1. pCAM105

   Plasmid

   #86479

   Purpose

   Expresses p9-FLAG-FMO

   Depositor

   Pamela Silver

   Article

   Myhrvold et al ACS Synth Biol. 2016 Aug 3.

   Insert

   p9-FLAG-FMO
   

   Use

   Synthetic Biology

   Tags

   FLAG and FMO

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAT205

   Plasmid

   #134882

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT220

   Plasmid

   #134888

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAS9i_TRP

   Plasmid

   #141253

   Purpose

   Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas12a

   Plasmid

   #186446

   Purpose

   Encodes Cas12a from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12a
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9_U6_sgRNA_Mettl3C

   Plasmid

   #165420

   Purpose

   sgRNA construct for targeting Mettl3 C-terminus

   Depositor

   Neil Brockdorff

   Article

   Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

   Insert

   PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCasX1

   Plasmid

   #87685

   Purpose

   p15A plasmid expressing CasX locus from Deltaproteobacteria

   Depositor

   Jennifer Doudna

   Article

   Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

   Insert

   CasX1
   

   Expression

   Bacterial

   Promoter

   None

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAM111

   Plasmid

   #86481

   Purpose

   Expresses p9-6His-tnaA

   Depositor

   Pamela Silver

   Article

   Myhrvold et al ACS Synth Biol. 2016 Aug 3.

   Insert

   p9-6His-tnaA
   

   Use

   Synthetic Biology

   Tags

   6His and tnaA

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCA0.421

   Plasmid

   #203950

   Purpose

   Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position

   Depositor

   Alistair McCormick

   Article

   Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCast

    Plasmid

    #200169

    Purpose

    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

    Depositor

    Yong-Su Jin

    Article

    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas2C

    Plasmid

    #82374

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas34

    Plasmid

    #82386

    Purpose

    sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAT105

    Plasmid

    #180510

    Purpose

    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas9CR5-NG

    Plasmid

    #120228

    Purpose

    pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.

    Depositor

    Christopher Reisch

    Insert

    SpCas9-NG
    

    Use

    Synthetic Biology

    Mutation

    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

    Promoter

    pTet

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAT202

    Plasmid

    #134879

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    deletion of Y208

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG:ECFP

    Plasmid

    #32597

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

    Insert

    ECFP
    

    Expression

    Mammalian

    Available Since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pCAGGS_2paCRY2

    Plasmid

    #129651

    Purpose

    2P-activatable CRY2

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCARSF63

    Plasmid

    #64695

    Purpose

    H6-TRX-Smt3-GB1-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Tags

    CBD, HisTag, NpuDnaE-N102, Smt3, and TRX

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCA_e-2

    Plasmid

    #202097

    Purpose

    Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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