We narrowed to 5,482 results for: pcas

1. pCas9CR5-NG

   Plasmid

   #120228

   Purpose

   pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.

   Depositor

   Christopher Reisch

   Insert

   SpCas9-NG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

   Promoter

   pTet

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCasX1

   Plasmid

   #87685

   Purpose

   p15A plasmid expressing CasX locus from Deltaproteobacteria

   Depositor

   Jennifer Doudna

   Article

   Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

   Insert

   CasX1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas1+2

   Plasmid

   #72676

   Purpose

   Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

   Depositor

   Udi Qimron

   Article

   Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

   Insert

   Cas1, Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT105

   Plasmid

   #180510

   Purpose

   Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   DpbCasX with R3 loop
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and U6

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS_2paCRY2

   Plasmid

   #129651

   Purpose

   2P-activatable CRY2

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG:ECFP

   Plasmid

   #32597

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Hadjantonakis et al BMC Biotechnol. 2002 Jun 11;2:11.

   Insert

   ECFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pCA_e-2

   Plasmid

   #202097

   Purpose

   Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCas_2+5

   Plasmid

   #60594

   Purpose

   encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cas_2+5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAT217

   Plasmid

   #134885

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCas49

    Plasmid

    #82391

    Purpose

    rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    rbcL-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    PccmK2

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas4_1_2_VA

    Plasmid

    #186438

    Purpose

    Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCARSF63

    Plasmid

    #64695

    Purpose

    H6-TRX-Smt3-GB1-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Use

    Tags

    CBD, HisTag, NpuDnaE-N102, Smt3, and TRX

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGmRuby2smFPMyc

    Plasmid

    #71816

    Purpose

    encodes multiple copies of an epitope tag Myc ( not detected by anti GFP ab)

    Depositor

    Loren Looger

    Insert

    mRuby2_smFP_Myc
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG:Kaede

    Plasmid

    #39261

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

    Insert

    Kaede
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCAT024

    Plasmid

    #171636

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BFP
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and U6

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGImC_Empty

    Plasmid

    #92015

    Purpose

    Empty backbone, includes IRES-mCherry

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMBPCas2/3

    Plasmid

    #89238

    Purpose

    Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

    Depositor

    Blake Wiedenheft

    Article

    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

    Insert

    Cas2/3
    

    Use

    Tags

    Strep II-MBP

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9CR4-NG

    Plasmid

    #120225

    Purpose

    pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.

    Depositor

    Christopher Reisch

    Insert

    SpCas9-NG
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Mutation

    Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

    Promoter

    pTet

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas3-Sm

    Plasmid

    #208001

    Purpose

    Expresses all necessary components of Type I-C CRISPR-Cas system

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Inserts

    RhaR (rhaR )
    RhaS (rhaS )
    pRhaBAD
    I-C crRNA
    Cas3
    Cas5
    Cas8
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCas1_Cas2/3

    Plasmid

    #89240

    Purpose

    Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

    Depositor

    Blake Wiedenheft

    Article

    Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

    Insert

    Cas1 and Cas2/3
    

    Use

    Tags

    Strep II tag on Cas1

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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