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We narrowed to 6,530 results for: nls

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Showing: 3161 - 3200 of 6530 results

  1. iPEmax-WT

    Plasmid
    #239385
    Purpose
    For inverse prime editor using Cas9-R221K-N394K in HEK294T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    Cas9-R221K-N393K, M-MLV-RT
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    R221K, N394K in Cas9
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239385-plasmid-map-sequence-id-481781

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  2. iPEmax-D10A

    Plasmid
    #239386
    Purpose
    For inverse prime editor using nCas9-D10A-R221K-N394K in HEK295T cells
    Depositor
    Caixia Gao
    Article
    Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:
    Insert
    nCas9-D10A-R221K-N394K, M-MLV-RT
    Use
    CRISPR
    Tags
    BPNLS and SV40 NLS, c-myc NLS
    Expression
    Mammalian
    Mutation
    D10A, R221K, N394K in Cas9
    Promoter
    CMV
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239386-plasmid-map-sequence-id-481780

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  10. SpCas9-NRCH-CGBE1

    Plasmid
    #198557
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-CGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198557-plasmid-map-sequence-id-388917

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  29. BPK4387 - human expression plasmid for SpCas9 Cluster 5

    Plasmid
    #101191
    Purpose
    Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster5(K918A/V922A/R925A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K918A/V922A/R925A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101191-plasmid-map-sequence-id-194254

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  30. MMW3645 - human expression plasmid for SpCas9 Cluster 3

    Plasmid
    #101187
    Purpose
    Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster3(T657A/G658A/W659A/R661A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    T657A/G658A/W659A/R661A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101187-plasmid-map-sequence-id-194250

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  31. MMW3770 - human expression plasmid for SpCas9 Cluster 4

    Plasmid
    #101189
    Purpose
    Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG
    Depositor
    Jennifer Doudna, Keith Joung
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-Cluster4(F491A/M495A/T496A/N497A)
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    F491A/M495A/T496A/N497A
    Promoter
    CMV
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101189-plasmid-map-sequence-id-194252

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  38. STEME-1

    Plasmid
    #138137
    Purpose
    Targeted simultaneous C-to-T and A-to-G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
    Expression
    Plant
    Available Since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    138137-plasmid-map-sequence-id-320280

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  39. A3A-PBE-NG

    Plasmid
    #138141
    Purpose
    Targeted C-to-T in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    APOBEC3A-nCas9-NG-UGI-NLS
    Expression
    Plant
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138141-plasmid-map-sequence-id-311740

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  40. pAdx-CMV-YFP

    Plasmid
    #73348
    Purpose
    Express YFP-NLS under CMV promoter
    Depositor
    Kazuhiro Oka
    Insert
    CMV-YFP
    Use
    Adenoviral
    Promoter
    CMV
    Available Since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73348-plasmid-map-sequence-id-138095

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Showing: 3161 - 3200 of 6530 results