We narrowed to 5,867 results for: nls

1. PX382 Staphylococcus pseudintermedius Cas9

   Plasmid

   #68319

   Purpose

   Staphylococcus pseudintermedius Cas9

   Depositor

   Feng Zhang

   Insert

   SpsCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX386 Lactobacillus farciminis Cas9

   Plasmid

   #68323

   Purpose

   Lactobacillus farciminis Cas9

   Depositor

   Feng Zhang

   Insert

   LfCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX395 Roseburia intestinalis Cas9

   Plasmid

   #68332

   Purpose

   Roseburia intestinalis Cas9

   Depositor

   Feng Zhang

   Insert

   RiCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX393 Gluconacetobacter diazotrophicus Cas9

   Plasmid

   #68330

   Purpose

   Gluconacetobacter diazotrophicus Cas9

   Depositor

   Feng Zhang

   Insert

   GdCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. AmCyan-P2A-Xerry

   Plasmid

   #48686

   Purpose

   Expresses AmCyan while the mCherry gene has been mutated

   Depositor

   Ilpo Huhtaniemi

   Insert

   AmCyan
   

   Use

   Tags

   mutant mCherry (truncated)

   Expression

   Mammalian

   Mutation

   SV40 NLS

   Promoter

   CMV

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX389 Fluviicola taffensis Cas9

   Plasmid

   #68326

   Purpose

   Fluviicola taffensis Cas9

   Depositor

   Feng Zhang

   Insert

   FtCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX388 Bacteroides coprophilus Cas9

   Plasmid

   #68325

   Purpose

   Bacteroides coprophilus Cas9

   Depositor

   Feng Zhang

   Insert

   BcCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PX378 Sutterella wadsworthensis Cas9

   Plasmid

   #68315

   Purpose

   Sutterella wadsworthensis Cas9

   Depositor

   Feng Zhang

   Insert

   SwCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PX387 Mycoplasma mobile Cas9

   Plasmid

   #68324

   Purpose

   Mycoplasma mobile Cas9

   Depositor

   Feng Zhang

   Insert

   MmCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PX392 Azospirillum B510 Cas9

    Plasmid

    #68329

    Purpose

    Azospirillum B510 Cas9

    Depositor

    Feng Zhang

    Insert

    AzoCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. SGEP-sh-Hs-PHGDH-67

    Plasmid

    #188671

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SGEP-sh-Hs-PHGDH-1968

    Plasmid

    #188673

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SGEP-sh-Hs-PHGDH-1957

    Plasmid

    #188674

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ipUSEPR-sg-Hs-PHGDH-2

    Plasmid

    #188676

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SGEP-sh-Hs-PHGDH-1967

    Plasmid

    #188672

    Purpose

    shRNA

    Depositor

    Lewis Cantley

    Insert

    shPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ipUSEPR-sg-Hs-PHGDH-5

    Plasmid

    #188679

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ipUSEPR-sg-Hs-PHGDH-6

    Plasmid

    #188680

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ipUSEPR-sg-Hs-PHGDH-4

    Plasmid

    #188678

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ipUSEPR-sg-Hs-PHGDH-3

    Plasmid

    #188677

    Purpose

    sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTagRFP-T2-PCNA-19

    Plasmid

    #58043

    Purpose

    Localization: Nucleus, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Insert

    PCNA (PCNA Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. PX380 Treponema denticola Cas9

    Plasmid

    #68317

    Purpose

    Treponema denticola Cas9

    Depositor

    Feng Zhang

    Insert

    TdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSL1180-HR-PUbECFP

    Plasmid

    #47917

    Purpose

    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

    Depositor

    Leslie Vosshall

    Insert

    ECFP
    

    Use

    Tags

    NLS

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti polyubiquitin (PUb)

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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24. pSBtet-RB-PE2

    Plasmid

    #173903

    Purpose

    SB-transposon with inducible expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TC9nickpAB

    Plasmid

    #71311

    Purpose

    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9 nickase
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    D10A nickase

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ipUSEPR-sg-Hs-PHGDH-int2

    Plasmid

    #188682

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH intron control (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ipUSEPR-sg-Hs-PHGDH-int1

    Plasmid

    #188681

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH intron control (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ipUSEPR-sg-Hs-PHGDH-int3

    Plasmid

    #188683

    Purpose

    control sgRNA

    Depositor

    Lewis Cantley

    Insert

    sgPHGDH intron control (PHGDH Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSBbi-RB-PE2

    Plasmid

    #173902

    Purpose

    SB-transposon with constitutive expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR-UCOE-SFFV-Zim3-dCas9-P2A-EGFP

    Plasmid

    #188899

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS, P2A-GFP, and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

    Plasmid

    #188902

    Purpose

    dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-KRAB(Kox1)-MeCP2
    

    Use

    Lentiviral

    Tags

    NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRL_CT_GI_del1

    Plasmid

    #98744

    Purpose

    For red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

    Inserts

    phytochrome interacting factor 3 (PIF3 Mustard Weed)
    phytochrome B (PHYB Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    SV40-NLS, VP64-AD, and synTALE-DBD

    Expression

    Yeast

    Mutation

    mutated nucleotide 576 C to A, deleted amino acid…

    Promoter

    FBA1 and TDH3

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. hV1aR-GFP

    Plasmid

    #67846

    Purpose

    mammalian expression of human V1a receptor with GFP tag

    Depositor

    Christian Gruber

    Article

    Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

    Insert

    V1a receptor (AVPR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

35. hV1b-GFP-1

    Plasmid

    #67847

    Purpose

    mammalian expression of human V1b receptor with GFP tag

    Depositor

    Christian Gruber

    Article

    Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

    Insert

    V1b receptor (AVPR1B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pM2AH + Slo1

    Plasmid

    #16195

    Depositor

    Larry Salkoff

    Article

    McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

    Insert

    calcium-activated potassium channel (KCNMA1, Kcnma1 Mouse, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PX458-3xHA-FnCas9

    Plasmid

    #130969

    Purpose

    3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    FnCas9
    

    Use

    CRISPR

    Tags

    3xHA-NLS and NLS-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYUU

    Plasmid

    #159751

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid

    #220611

    Purpose

    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMS73

    Plasmid

    #110629

    Purpose

    CRISPR/Cas9 vector for mutliplexed genome editing in Ustilago maydis. Expresses U. maydis codon-optimized Cas9 under the hsp70 promoter, contains a short version of U6 promoter, is self-replicating

    Depositor

    Regine Kahmann

    Article

    Schuster et al Fungal Genet Biol. 2018 Mar;112:21-30. d

    Inserts

    short U6 promoter
    cas9
    tnos terminator
    

    Use

    CRISPR; Self-replicating in ustilago maydis, conf…

    Tags

    N-terminal NLS, C-terminal HA-tag +NLS

    Expression

    Bacterial

    Mutation

    Streptococcus pyogenes gene codon-optimized for U…

    Promoter

    U. maydis hsp70 promoter (Kronstad and Leong, 198…

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge