We narrowed to 5,563 results for: SPI
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Plasmid#145577PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAd-Track SIRT1 H355A
Plasmid#8441DepositorInsertSIRT1 (Sirt1 Mouse)
UseAdenoviralTagsExpressionMammalianMutationHistidine 355 mutated to AlaninePromoterAvailable sinceDec. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLacI-LAP-BUB1
Plasmid#115905PurposeUsed to express BUB1-WT fused to LacI for recruitment of BUB1 to LacO spots.DepositorInsertLacI-BUB1 (BUB1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Plexin D1 in situ probe
Plasmid#45621DepositorInsertPlexin D1 in situ probe (Plxnd1 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 5751-6406 of NM_026376.3PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-75-2
Plasmid#73036PurposeExpress an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cellsDepositorInsertp75-2
UseLentiviralTagsExpressionMammalianMutationMature Human NT-3 gene exchanged Aspartic 15 to a…PromoterAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RV
Plasmid#188544PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editingDepositorInsertLbCas12a-RV
UseCRISPR; Gateway compatible lbcas12a-rv entry cloneTagsNLSExpressionPlantMutationG146R, R182VPromoterAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
CoV-NSP3-c001
Plasmid#173082PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep
UseTagsmCeruleanExpressionBacterialMutationE1000-K1173, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGT8162 (AATG_dttLbCas12a-i_TTCG)
Plasmid#192350PurposeL0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expressionDepositorInsertAATG_dttLbCas12a-i_TTCG
UseCRISPRTagsExpressionMutationD156R; E925QPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-1
Plasmid#126053PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pY015 (pcDNA3.1-hLiCpf1)
Plasmid#69987PurposeExpresses humanized LiCpf1DepositorInserthLiCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046940
Plasmid#149261PurposeBacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
PSUC2-yEGFPCLN2PEST-pRS406
Plasmid#64405PurposeSUC2 promoter driven GFP expressionDepositorInsertSUC2 promoter (SUC2 Budding Yeast)
UseTagsyEGFPCLN2PEST (destabilized yeast EGFP)ExpressionYeastMutationPromoterSUC2Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-Pds1Δdestruction box(383)
Plasmid#39848DepositorInsertPds1 (PTTG1 Human)
UseTagsEYFPExpressionMammalianMutationΔdestruction box = ΔR61-N68PromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJKW1813
Plasmid#174003PurposeExpresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeastDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry2-sgC9cen
Plasmid#198326PurposeExpresses a guide RNA against a repetitive locus found in the pericentromere of human chromosome 9, with mCherry2 reporterDepositorInsertChr9-CEN guide RNA
UseTagsExpressionMammalianMutationPromoterhU6Available sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK158_mCherry-4.1G-deltaCTD-hardened
Plasmid#46360DepositorInsert4.1G (EPB41L2 Human)
UseTagsmCherryExpressionMammalianMutationDeleted amino acids 886-1005, and modified for si…PromoterCMVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_C1 I3
Plasmid#61397PurposeExpresses GFP-tagged Inhibitor-3 in mammalian cellsDepositorInsertInhibitor-3 (PPP1R11 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIW086
Plasmid#134132PurposeBacterial expression of His-TEV-Ska1 and Ska2DepositorInsertHis-TEV-human Ska1 ; human Ska2
UseTagsHis-TEVExpressionBacterialMutationK183A K184APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVenus_Lys-less-Cdc20 (1620)
Plasmid#39849DepositorInsertCdc20 (CDC20 Human)
UseTagsVenusExpressionMammalianMutationLys-less = all Lys converted to ArgPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5-MIS12-LAP-BUB1
Plasmid#115904PurposeUsed for expression of siRNA-resistant MIS12-BUB1 from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertMIS12-BUB1 (MIS12 Human)
UseTagsEYFPExpressionMammalianMutationBUB1 fused to MIS12PromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo S100a10 in situ probe
Plasmid#45603DepositorInserts100a10 in situ probe (S100a10 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 21-275 of BC025044PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
Venus-Cdc20 R132A (1768)
Plasmid#26059DepositorInsertCdc20 (CDC20 Human)
UseTagsVenusExpressionMammalianMutationMutation in the Mad2 binding site, R132APromoterAvailable sinceAug. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
1kb Zfp521 promoter reporter
Plasmid#104188Purposeluciferase reporter to assay Zfp521 promoter activationDepositorInsertZfp521 promoter - 1 Kb (Zfp521 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoternoneAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046938
Plasmid#145599PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTK157_mCherry-4.1G-CTD
Plasmid#46359DepositorInsert4.1G C-terminal domain (EPB41L2 Human)
UseTagsmCherryExpressionMammalianMutationC-terminal domain from 886-1005 amino acidsPromoterCMVAvailable sinceJuly 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Foxp2 in situ probe
Plasmid#45620DepositorInsertFoxp2 in situ probe (Foxp2 Mouse)
UseIn situTagsExpressionMutationfragment contains bp#1709-2142 of AF339106PromoterAvailable sinceJuly 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST
Plasmid#175017PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertOK-HEFwtED
UseTagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGN33
Plasmid#131105PurposeAn integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutantsDepositorInsertP-furA102tetO-pip
UseTagsExpressionBacterialMutationthe furA102 promoter was mutated with the inserti…PromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEM01
Plasmid#135414PurposeExpression of Hygromycin resistance from CMV promoterDepositorInsertCMVpr hph ADH1t
UseTagsExpressionBacterial and YeastMutationPromoterCMVprAvailable sinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Cux2 in situ probe
Plasmid#45623DepositorInsertCux2 in situ probe (Cux2 Mouse)
UseIn situTagsExpressionMutationfragment contains nt 1069-1694 on NM_007804PromoterAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046831
Plasmid#145671PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;MycExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Ncerulean-PHdomain
Plasmid#66784PurposeExpression of PIP2 binding protein in sea urchin embryosDepositorInsertPLCdelta (PLCD1 Human)
UseSea urchin, zebrafish, xenopusTagsCeruleanExpressionMammalianMutationonly contains PH domainPromoterSP6Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
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NmCitrine-PHdomain
Plasmid#66783PurposeExpression of PIP2 binding protein in sea urchin embryosDepositorInsertPLCdelta (PLCD1 Human)
UseSea urchin, zebrafish, xenopusTagsmCitrineExpressionMammalianMutationonly contains PH domainPromoterSP6Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-3
Plasmid#126054PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x3 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-8A/S55D
Plasmid#114032PurposeInducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell linesDepositorInserteGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 8A/S55DPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo Crym in situ probe
Plasmid#45601DepositorInsertCrym in situ probe (Crym Mouse)
UseIn situTagsExpressionMutationfragment contains nt 756-1191 of NM_016669PromoterAvailable sinceJune 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVRa37_3390
Plasmid#49697DepositorInsertECF37_3390
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQE9-His-p97deltaD2(K251A)
Plasmid#17230DepositorInsertp97 (Vcp Mouse)
UseTagsHisExpressionBacterialMutationchange codon 457 to stop codon, change lysine 25…PromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHR-PUM.HD-mCherry-Cry2WT
Plasmid#240102PurposeMammalian lentiviral vector for expressing the mCherry-tagged Pumilio homology domain fused to the light-inducible oligomerization domain CRY2.DepositorInsertPumilio homology domain
UseLentiviralTagsmCherry and Cry2ExpressionBacterial and MammalianMutationPromoterSFFV promoterAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
NP-11-63
Plasmid#234358PurposeExpressing and purifying R.rubrum rubisco using a his-sumo tagDepositorInsertR.rubrum rubisco
UseSynthetic BiologyTagsN-term SUMO tagExpressionMutationPromoterT7Available sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
MPS1 Human
Plasmid#232149PurposeMPS1 from Human codon optimized for S. cerevisiae in pUCIDTDepositorInsertMPS1 (TTK Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
ECMAB - pET22b Ag43 160N 6H Mms6
Plasmid#225539PurposeConstruction of extracellular magnetite accumulating bacterial systems.DepositorInsertsIron binding protein
Autotransporter protein
UseTagsnoExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
LVDP 2P.2.EGFP
Plasmid#231905PurposeFor the production of SARS-CoV-2 virus-like particles (VLPs) in the '2-plasmid' system and packaging EGFP mRNA into the VLPsDepositorUseLentiviralTagsExpressionMutationR203M in N proteinPromoterCMV, EF-1aAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
EYFP-Parkin
Plasmid#210292Purposemitophagy markerDepositorInsertEYFP-Parkin
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mActA-FKBP-mCherry
Plasmid#210295PurposeActuAtor for FKBP-FRB CIDDepositorInsertmActA-FKBP-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
ECFP-FRB-GiantinS
Plasmid#210296PurposeGolgi marker and anchor for FKBP-FRB CIDDepositorInsertECFP-FRB-GiantinS
UseTagsECFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EYFP-FKBP-DN-N-WASP
Plasmid#210301Purposecontrol for targeted actin nucleationDepositorInsertEYFP-FKBP-DN-N-WASP
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EYFP-FKBP-N-WASP
Plasmid#210302Purposetargeted actin nucleationDepositorInsertEYFP-FKBP-N-WASP
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only