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We narrowed to 18,759 results for: nls

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Showing: 3441 - 3480 of 18759 results

  4. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

    Plasmid
    #107301
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
    Use
    CRISPR
    Tags
    FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107301-plasmid-map-sequence-id-204785

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  9. pCM33

    Plasmid
    #261120
    Purpose
    For C-terminal tagging a protein of interest with VC155 (split-YFP) and mCherry expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    rgef-1p::AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    rgef-1
    Available since
    Oct. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    261120-plasmid-map-sequence-id-523184

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  10. pCM39

    Plasmid
    #261126
    Purpose
    For C-terminal a tagging protein of interest with VN173 (split-YFP) and mTagBFP expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    myo-3p::AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    myo-3
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    261126-plasmid-map-sequence-id-520972

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  11. pCM38

    Plasmid
    #261125
    Purpose
    For C-terminal a tagging protein of interest with VC155 (split-YFP) and mCherry expression under myo-3 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    myo-3p::AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    myo-3
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261125-plasmid-map-sequence-id-520103

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  12. pCM64

    Plasmid
    #261134
    Purpose
    For C-terminal a tagging protein of interest with VC155 (split-YFP) and mCherry expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    elt-2p::AID::nls::mcherry::2a::AID::VC155::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    elt-2 minimal promoter
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261134-plasmid-map-sequence-id-520101

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  13. pCM70

    Plasmid
    #261135
    Purpose
    For N-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression under elt-2 minimal promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    elt-2p::AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    elt-2 minimal promoter
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261135-plasmid-map-sequence-id-520100

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  14. pCM34

    Plasmid
    #261121
    Purpose
    For C-terminal tagging a protein of interest with VN173 (split-YFP) and mTagBFP expression under rgef-1 promoter expression. Can be integrated at single-copy into genome using RMCE.
    Depositor
    John Calarco
    Publication
    Martin et al bioRxiv 2025.02.28.640866
    Insert
    rgef-1p::AID::nls::mTagbfp2::2a::AID::VN173::tbb-2 3' in pLF3FShC
    Expression
    Worm
    Promoter
    rgef-1
    Available since
    Aug. 31, 2026
    Availability
    Academic Institutions and Nonprofits only
    261121-plasmid-map-sequence-id-520093

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  15. deltaVP1 + VP2C-MIOX

    Plasmid
    #166680
    Purpose
    Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Publication
    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-
    Inserts
    VP2C-MIOX
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available since
    April 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    166680-plasmid-map-sequence-id-329927

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  18. RANLS-IETD-BNES

    Plasmid
    #60972
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A and ddRFP B
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pCMV-ACBEmax

    Plasmid
    #139106
    Purpose
    Mammalian ACBEmax expression vector (pSI-632)
    Depositor
    Nozomu Yachie
    Publication
    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    139106-plasmid-map-sequence-id-273273

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  24. pcDNA3-Myc:P4:gs:nls:ABI

    Plasmid
    #135985
    Purpose
    Expresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P4 coiled-coil fused to the ABI domain
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135985-plasmid-map-sequence-id-268595

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  26. pAAV_hSyn_TALEBB(NI)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

    Plasmid
    #47452
    Purpose
    AAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NI_C63)-SID4X
    Use
    AAV; Tale
    Tags
    2A_phiLOV2.1
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47452-plasmid-map-sequence-id-72981

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  27. pAAV_hSyn_TALEBB(NN)-NLS-VP64_2A_GFP_WPRE_bGHpA

    Plasmid
    #47445
    Purpose
    AAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature
    Insert
    TALE BB(N136_0.5NN_C63)-VP64
    Use
    AAV; Tale
    Tags
    2A_GFP
    Expression
    Mammalian
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available since
    Oct. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    47445-plasmid-map-sequence-id-72977

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  28. MSP1594_2x_NLS

    Plasmid
    #110625
    Purpose
    Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    St1Cas9 LMD-9
    None
    Use
    CRISPR and Synthetic Biology
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    110625-plasmid-map-sequence-id-208000

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  29. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

    Plasmid
    #114079
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114079-plasmid-map-sequence-id-231032

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  32. pMZ-176

    Plasmid
    #195424
    Purpose
    Transient, constitutive expression of synZiFTR: ZF1-p65
    Depositor
    Ahmad Khalil
    Publication
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pUb - 3XFLAG - NLS - ZF1-3 (ZF1) - p65 - RbGpA
    Expression
    Mammalian
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195424-plasmid-map-sequence-id-385003

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  34. 868 pSG5L HA NLS PTEN G129R

    Plasmid
    #10935
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    G129R
    Available since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 3441 - 3480 of 18759 results