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We narrowed to 6,273 results for: nls

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Showing: 3441 - 3480 of 6273 results

  4. hnRNPA1_allW_D262V-FLAG-IRES-eGFP

    Plasmid
    #234621
    Purpose
    Mammalian expression of full-length hnRNPA1 D262V with all aromatic amino acids in the C-terminal LCD mutated to W keeping the hexapeptide fibril core (SYNDFG) and the PY-NLS intact.
    Depositor
    Tanja Mittag
    Article
    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2
    Insert
    hnRNPA1 allW D262V (HNRNPA1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Famillial ALS mutation D262V, all aromatic amino …
    Promoter
    CMV
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    234621-plasmid-map-sequence-id-464954

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  7. pCUU

    Plasmid
    #159747
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159747-plasmid-map-sequence-id-313815

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  8. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid
    #196720
    Purpose
    Tet-inducible expression of Cas9-T2A-TagBFP
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196720-plasmid-map-sequence-id-389756

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  9. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

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  10. pCMV-ABE-P48R

    Plasmid
    #161816
    Purpose
    Expresses ABE-P48R in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    161816-plasmid-map-sequence-id-320046

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  11. HaloTag-LacI

    Plasmid
    #232636
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232636-plasmid-map-sequence-id-462868

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  12. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid
    #196722
    Purpose
    Tet-inducible expression of Cas9-T2A-miRFP670nano
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196722-plasmid-map-sequence-id-389804

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  13. pHygRB12

    Plasmid
    #244899
    Purpose
    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attB site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244899-plasmid-map-sequence-id-485995

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  14. pHygGB16

    Plasmid
    #244896
    Purpose
    hygR selection nls-GFP marked rapid RMCE integration vector with an attB site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244896-plasmid-map-sequence-id-485985

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  15. pHygGP16

    Plasmid
    #244903
    Purpose
    hygR selection nls-GFP marked rapid RMCE integration vector with an attP site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244903-plasmid-map-sequence-id-485493

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  16. pHygRP12

    Plasmid
    #244905
    Purpose
    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attP site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244905-plasmid-map-sequence-id-485485

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  18. pb-CAGGS-dCas9-blasto

    Plasmid
    #218555
    Purpose
    Encodes CAG promoter-driven dCas9 with a C-terminal bipartite SV40 NLS, along with blasticidin resistance
    Depositor
    Alejandro Chavez
    Insert
    dCas9
    Use
    CRISPR; Piggybac transposition
    Tags
    2xSV40NLS
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218555-plasmid-map-sequence-id-431930

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  20. PHGDH

    Plasmid
    #38841
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHGDH (PHGDH Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid
    #68336
    Purpose
    Campylobacter lari CF89-12 Cas9
    Depositor
    Feng Zhang
    Insert
    ClCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68336-plasmid-map-sequence-id-119558

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  24. pHygRB2

    Plasmid
    #244897
    Purpose
    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attB site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244897-plasmid-map-sequence-id-485986

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  25. pHyGB6

    Plasmid
    #244894
    Purpose
    hygR selection nls-GFP marked rapid RMCE integration vector with an attB site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244894-plasmid-map-sequence-id-485981

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  26. pHygGP6

    Plasmid
    #244902
    Purpose
    hygR selection nls-GFP marked rapid RMCE integration vector with an attP site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244902-plasmid-map-sequence-id-485494

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  27. pHygRP2

    Plasmid
    #244904
    Purpose
    hygR selection nls-Scarlet marked rapid RMCE integration vector with an attP site for RMHE
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Recombination mediated cassette exchange; recombi…
    Expression
    Worm
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244904-plasmid-map-sequence-id-485487

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  28. pdCas9-tdTomato

    Plasmid
    #232631
    Purpose
    catalytically inactive dCas9 for localization; labeled with tdTomato
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV (TATA box removed)
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232631-plasmid-map-sequence-id-462150

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  36. PX458-3xHA-en31FnCas9

    Plasmid
    #201949
    Purpose
    Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Debojyoti Chakraborty
    Article
    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:
    Insert
    3xHA-NLS-en31FnCas9-T2A-EGFP
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    G1243T, E1369R, E1603H on FnCas9
    Promoter
    Cbh
    Available Since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    201949-plasmid-map-sequence-id-405303

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  37. TDP-REGv2 Triple Cryptic Cre recombinase in pTwist-CMV

    Plasmid
    #216162
    Purpose
    Expresses Cre recombinase in cells lacking TDP-43 function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    Cre recombinase with N-terminal SV40 NLS and C-terminal c-Myc NLS
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216162-plasmid-map-sequence-id-437755

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  40. pYEE

    Plasmid
    #159750
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159750-plasmid-map-sequence-id-313819

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Showing: 3441 - 3480 of 6273 results