We narrowed to 7,680 results for: CCH

Showing: 4301 - 4350 of 7680 results

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pNeoXTR f1

Plasmid

#69158

Purpose

mouse targeting vector

Depositor

David Feldser

Article

Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

Inserts

DTA
NeoR
GFP
AmpR

Use

Mouse Targeting

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fSNAP-3xHA

Plasmid

#188935

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fSNAP-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pIM23

Plasmid

#74643

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

MET25

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB8786

Plasmid

#126920

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCLX-UBI-mCherry

Plasmid

#114275

Purpose

Ubiquitous expression of the mCherry living color

Depositor

Patrick Salmon

Article

Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

Insert

mCherry

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Osh1

Plasmid

#58835

Purpose

Expresses GFP-Tagged Osh1 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Osh1 (SWH1, OSH1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

PHO5

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pHACK(Gal4)-DONR(T2A-Flp1)

Plasmid

#194770

Purpose

To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.

Depositor

Chun Han

Article

Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

Insert

T2A-Flp1

Use

CRISPR

Expression

Insect

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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DT035A

Plasmid

#159397

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Promoter

tac promoter

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-I-SceI

Plasmid

#165041

Purpose

Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

I-SceI

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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ESCRT-II

Plasmid

#17633

Depositor

James Hurley

Article

Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

Insert

pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pHES836

Plasmid

#89195

Purpose

Expresses mKate2 under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-mKate2

Expression

Yeast

Promoter

pZ

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pNeoXTR f2

Plasmid

#69159

Purpose

mouse targeting vector

Depositor

David Feldser

Article

Robles-Oteiza et al Nat Commun. 2015 Nov 5;6:8783. doi: 10.1

Inserts

DTA
NeoR
GFP
AmpR

Use

Mouse Targeting

Available Since

Dec. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pET21c-eIF4A

Plasmid

#37230

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF1 (TIF1 Budding Yeast)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
p404-Grx5-GFP1-10

Plasmid

#91952

Purpose

Expresses GFP1-10 in the mitochondrial matrix

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

Grx5-GFP1-10

Expression

Yeast

Promoter

GAP

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pZZMP154

Plasmid

#231760

Purpose

Mammalian expression of LZ-SPO11

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

LZ-SPO11 (Spo11 Mouse, Budding Yeast)

Tags

Flag-LeucineZipper

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pMAL728

Plasmid

#169152

Purpose

Encodes OptoQ-AMP6

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pSMF2

Plasmid

#112819

Purpose

Expresses wild-type Rpb1 in yeast cells

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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LHP306 - Ub - no Lys

Plasmid

#32150

Depositor

Linda Hicke

Article

Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Expression

Yeast

Mutation

No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

Promoter

CUP1

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pRS426-GFP-Osh2-PH

Plasmid

#71373

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pTCW012

Plasmid

#83527

Purpose

Cloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminator

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

pFUS1J2

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Atg24 1B

Plasmid

#197046

Purpose

His6 tagged Atg24 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Reinhart et al Biochemistry. 2022 Apr 14. doi: 10.1021/

Insert

Atg24

Tags

6His-TEV

Expression

Bacterial

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCUPI NPD-GFP

Plasmid

#1191

Depositor

Susan Lindquist

Article

Patino et al Science 1996 Aug 2;273(5275):622-6.

Insert

N-terminus of Sup35 (SUP35 Budding Yeast)

Use

Cup1 promoter

Tags

GFP

Expression

Yeast

Mutation

fused N-terminal Sup35 (~1-253aa) to GFP

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pPOM033_P7_3"His5

Plasmid

#216458

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''his5

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM026_P2_pNMT1

Plasmid

#216451

Purpose

Regulatable promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter nmt1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM019_P2_pRPL2102

Plasmid

#216444

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rpl2102

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM015_P2_pADH1

Plasmid

#216440

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter adh1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM013_P2_pENO101

Plasmid

#216438

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter eno101

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
UBC13-PGEX4T

Plasmid

#25464

Depositor

Cynthia Wolberger

Article

Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

Insert

Ubc13 (UBC13, MMS2 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pCCL-ReDNAP (Y427A)

Plasmid

#52346

Purpose

CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

Recoded TP-DNAP

Expression

Bacterial and Yeast

Promoter

REV1

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-AVT1 chr I

Plasmid

#41898

Purpose

Yeast expression of AVT1.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

AVT1 (AVT1 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS101

Plasmid

#24631

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

FAA2 (FAA2 Budding Yeast)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
M2660 ura3::LYS2 Disruptor Converter

Plasmid

#51678

Purpose

Yeast marker swap plasmid for converting URA3 to LYS2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LYS2 (LYS2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBN449

Plasmid

#129555

Purpose

GAL4-induced expression of codon-optimised FLP recombinase with unfused mNeongreen (mNG) as a marker in C. elegans

Depositor

Peter Askjaer

Article

Ayuso et al MicroPubl Biol. 2019 Jan 29;2019:10.1791

Insert

15xUAS-∆pes-10-Flp-SL2

Tags

mNeonGreen

Expression

Worm

Promoter

UAS-∆pes-10

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-pomMet26

Plasmid

#107448

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met26 (met26 Fission Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-pomMet16

Plasmid

#107452

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met16 (met16 Fission Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRE57I-GNAc

Plasmid

#170637

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

Insert

Mini-Tn6677, GNAc cassette, lacI

Expression

Bacterial

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTYF-mCMV/GfaABC1D-EGFP

Plasmid

#19976

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMVGfaABC1D-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pZA2[PLac:GFP]

Plasmid

#60778

Purpose

Contains Plac driving expression of GFP

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

yemGFP

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-URA-GAL1-fCLIP-3xHA

Plasmid

#188936

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-3xHA

Expression

Yeast

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV10-3xFLAG-prs-MCP1

Plasmid

#184341

Purpose

Express yeast MCP1 in Expi293F cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Yeast MCP1 (MCP1 Budding Yeast)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
YIplac211-MNN9-msGFP2

Plasmid

#177105

Purpose

Expresses Mnn9-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

MNN9 (MNN9 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX2TK-Osh2-PH

Plasmid

#71370

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pIM700

Plasmid

#74639

Purpose

alias pFA6a-yTagRFP-T-natNT2 - vector for C-terminal tagging of yeast genes with yeast-adapted TagRFP-T and nourseothricin selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

none

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
EC2_7_dCas9_HC_sgRNA

Plasmid

#163711

Purpose

High copy plasmid with dCas9 and sgRNA expression cassettes

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

dCas9

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX2TK-Num1-PH

Plasmid

#71369

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
M3938 trp1::ADE2 Disruptor Converter

Plasmid

#51673

Purpose

Yeast marker swap plasmid for converting TRP1 to ADE2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

ADE2 (ADE2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Promoter

ADE2

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-3XHA-HALO-Kanamycin

Plasmid

#188933

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Kanamycin

Expression

Yeast

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHES822

Plasmid

#89193

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Expression

Yeast

Promoter

pZ

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,680 results for: CCH