163,606 results

1. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

   Plasmid

   #216250

   Purpose

   HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   HDR template for human SEC61B (SEC61B Human, HDR template, Synthetic)
   

   Use

   CRISPR; Hdr template

   Tags

   BSD.P2A.miniIAA7.3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAS9-mCherry-Frame +0

   Plasmid

   #66939

   Purpose

   CRISPaint frame selector +0

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   gRNA frame +0
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1227 pcDNA3 Myr HA Akt2

   Plasmid

   #9016

   Depositor

   William Sellers

   Insert

   Akt2 (AKT2 Human)
   

   Use

   Tags

   HA and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

   Plasmid

   #223125

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

   Plasmid

   #216244

   Purpose

   Express AtAFB2.F74A mutant with mCherry tag for AID2

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   AtAFB2.F74A-mCherry
   

   Use

   CRISPR, TALEN, and Unspecified ; Human safe harbo…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   F74A mutation

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. iCas

   Plasmid

   #84232

   Purpose

   Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

   Depositor

   Meng How Tan

   Article

   Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

   Insert

   Cas9
   

   Use

   Tags

   2A-OFP co-expression and ERT2-ERT2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP12918 - pAAV-AiE0459m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2918)

   Plasmid

   #224016

   Purpose

   AiE0459m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK345 (Hygro-P2A-mAID-mClover)

   Plasmid

   #121179

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mAID-mClover
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SF-SON IRES Puro

   Plasmid

   #214402

   Purpose

   Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

   Depositor

   Tanja Kuhlmann

   Article

   Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. GluA2 (px330:2-guideCas9)

    Plasmid

    #169420

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBXNH3CA_MBP

    Plasmid

    #132700

    Purpose

    expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Maltose binding Protein
    

    Use

    Tags

    Avi and His

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCer-C3-Plk1-K82R

    Plasmid

    #68134

    Purpose

    Expression of Cerulean-tagged, kinase-dead Plk1 in mammalian cells

    Depositor

    Catherine Lindon

    Insert

    Polo-like kinase 1 (PLK1 Human)
    

    Use

    Tags

    Cerulean

    Expression

    Mammalian

    Mutation

    K82R kinase-dead version

    Promoter

    CMV

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-myc-Cdc42-Q61L

    Plasmid

    #12974

    Depositor

    Gary Bokoch

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIB-HIS-TEV-scCAK1

    Plasmid

    #177014

    Purpose

    Generate baculovirus for insect cell expression of scCAK1 with N-terminal Polyhistidine-TEV

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CAK1 cyclin-dependent protein kinase-activating kinase CAK1 (CAK1 Budding Yeast)
    

    Use

    Tags

    Polyhistidine-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMD2-VSVGmut

    Plasmid

    #182229

    Purpose

    Expresses VSVGmut protein in mammalian cells for lentiviral pseudotyping

    Depositor

    Michael Birnbaum

    Article

    Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

    Insert

    VSVGmut
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47Q, R354A

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. iNos-eGFP

    Plasmid

    #207251

    Purpose

    Reporter for expression of eGFP under control of iNos promoter

    Depositor

    Michelle Farkas

    Article

    Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

    Insert

    iNos Promoter (Nos2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172318

    Purpose

    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-dCry-V5His

    Plasmid

    #136241

    Purpose

    Protein expression of D. melanogaster (Dm) cytochrome with V5 and His tags

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    DmCry
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMK349 (BSD-P2A-mAID-mCherry)

    Plasmid

    #121183

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pmCherry-N1-cofilin

    Plasmid

    #186750

    Purpose

    Expresses wildtype mCherry-tagged human cofilin in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

    Insert

    Cofilin-1 (CFL1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. Mucolipin1-pHcRed C1

    Plasmid

    #62959

    Purpose

    full length human Mucolipin-1 cloned into pHcRed1 C1

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Use

    Tags

    HcRed1

    Expression

    Mammalian

    Mutation

    Mucolipin-1 has 1 silent mutation compared to the…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLV-NT-3/D15A

    Plasmid

    #73035

    Purpose

    Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Insert

    NT-3/D15A (NTF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated Aspartic acid in position 15 of the matur…

    Promoter

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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23. Mucolipin1 D471-472K-pHcRed C1

    Plasmid

    #62961

    Purpose

    full length human Mucolipin-1 D471/472K cloned into pHcRed1 C1

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Use

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    D471/472K (and two silent mutations T77 ACA inst…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pCI-neo-sOVA

    Plasmid

    #25098

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    soluble ovalbumin
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEGFP VARP

    Plasmid

    #42312

    Depositor

    Thierry Galli

    Article

    Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

    Insert

    VARP (ANKRD27 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

26. PSD95-TS:YFP

    Plasmid

    #42225

    Depositor

    Roger Tsien

    Article

    Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

    Insert

    PSD95
    

    Use

    Tags

    TS:YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Ube2v2

    Plasmid

    #31430

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2V2 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

28. pMiniT:SEP-GluA2 TKIT donor

    Plasmid

    #169421

    Purpose

    Donor DNA for TKIT knockin of SEP to GluA2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Super ecliptic pHluorin (SEP)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CL20_mEGFP_EEF2_SMARCA4

    Plasmid

    #205808

    Purpose

    Express mEGFP-tagged fusion protein, EEF2_SMARCA4 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    EEF2_SMARCA4 (EEF2, SMARCA4 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

    Plasmid

    #175529

    Purpose

    Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Cox8a-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge