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pDONR-SUMO1
Plasmid#114388PurposepDONR vector with SUMO1 geneDepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionMammalianMutationQ29H compared with NCBI reference NP_003343.1PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEb
Plasmid#193832PurposeExpress TadCBEb (with SpCas9) in mammalian cellsDepositorInsertTadA-CDb-SpCas9 D10A-2xUGI
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-Lmo2
Plasmid#60666PurposePiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoterDepositorInsertLmo2 (Lmo2 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP ArgSen delta Rad23b UbL
Plasmid#184703PurposeLentiviral transfer plasmid for expression of GFP ArgSen delta Rad23b UbLDepositorInsertGFP ArgSen delta Rad23b UbL
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
sgCTRL-hygro
Plasmid#199639PurposesgRNA control; induces CAS9 cutting in an intergenic regionDepositorInsertN/A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pk_CyRPA-Cd4-bio-His
Plasmid#126841PurposeExpression of recombinant P. knowlesi protein in mammalian cellsDepositorInsertCyRPA (PKH_052740 Plasmodium knowlesi)
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-ONL
Plasmid#65713PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-ONL)DepositorInsert7xTcf, minCMV, 3xNLS, ONL
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PtW-eSpCas9
Plasmid#133357PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsHA-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-α1L9'S-nAchR
Plasmid#20684DepositorInsertnicotinic acetylcholine receptors (Chrna1 Mouse)
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
Myc-Foxo1-WT 6KR
Plasmid#17558DepositorInsertFoxo1 6KR (Foxo1 Mouse)
UseTagsMycExpressionMammalianMutationK242R, K246R, K259R, K262R, K271R, K291RPromoterCMVAvailable sinceOct. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEY61
Plasmid#191058Purposeacr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)DepositorInsertacr-2(3.7k) (acr-2 Nematode)
UseTagsExpressionWormMutationNonePromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-miR-148a
Plasmid#65052PurposeExpression of miR148a in mammalian cellsDepositorInserthsa-miR-148a (pre-miRNA) (MIR148A Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJT112_GalL_nCDA1-xBE3
Plasmid#141318PurposeExpressing base editor nCDA1-xBE3 in yeast cellsDepositorInsertnCDA1-xBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
p-miR-216a
Plasmid#65054PurposeExpression of miR216a in mammalian cellsDepositorInserthsa-miR-216a (pre-miRNA) (MIR216A Human)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
aav-U6-broccoli-hsp68-mCherry-MCS-pA
Plasmid#195411PurposeAAV9 based enhancer activity assayDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC234r-ccdB/TPK2
Plasmid#176179Purposeyeast MoClo level-0 part plasmid type 234r containing toxic gene dropout cassette (ccdB and TPK2)DepositorInsertdropout cassette containing toxic genes ccdB and TPK2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1820
Plasmid#154321PurposeMulti-cassette for SapTrapDepositorInsertGFP^SEC (LoxP)^AID*::3xFLAG
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
AtS/MAR10 insulator sequence (GB3458)
Plasmid#160638PurposepEGB3a1 AtS/MAR10DepositorInsertAtS/MAR10 insulator sequence
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJW1759
Plasmid#154315PurposeNT Donor-cassette for SapTrapDepositorInsert30aa linker::TEV::AID*::3xFLAG for NT slot
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
625_HOXD13 in pmiRGlo
Plasmid#78129Purposeluciferase reporter for miRNA activityDepositorInsertHOXD13 (HOXD13 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation3'UTR containing miRNA binding sitesPromoterPGKAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nuc-Orange Nano-lantern(Ca2+)/pcDNA3
Plasmid#71349PurposeExpress Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian celDepositorInsertOrange Nano-lantern(Ca2+)_H2B
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
QuasAr-P2A-GCaMP
Plasmid#154082PurposeIndependent expression of QuasAr and GCaMP under human synapsin 1 promoterDepositorInsertQuasAr-P2A-GCaMP6f
UseAAVTagsExpressionMammalianMutationChanged Tyrosine 327 to AlaninePromoterhuman synapsin 1Available sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1806
Plasmid#154319PurposeNT Donor-cassette for SapTrapDepositorInsertlinker::3xMyc for NT slot
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1805
Plasmid#154318PurposeCT Donor-cassette for SapTrapDepositorInsertlinker::3xMyc for CT slot
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1348
Plasmid#154311PurposeNT Donor-cassette for SapTrapDepositorInsert30aa linker for NT slot
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1822
Plasmid#154322PurposeMulti-cassette for SapTrapDepositorInsert30aa linker::GFP
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mito-Cyan Nano-lantern(Ca2+)/pcDNA3
Plasmid#71345PurposeExpress Mito-Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian celDepositorInserthOTC_Cyan Nano-lantern(Ca2+)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-β1L9'S-nAchR
Plasmid#20685DepositorInsertnicotinic acetylcholine receptors (Chrnb1 Mouse)
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(1-114)
Plasmid#99513PurposeTruncated C-terminal AID degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(1-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTagsExpressionMutationinternal inversion of nucleotides 265-312 of the …PromoterAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR FOXP3 WWRR
Plasmid#16364DepositorInsertFOXP3 (FOXP3 Human)
UseTagsExpressionBacterialMutationWWRR: T359W, N361W, E399R, E401RPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBS-AMV-γ1L9'S-nAchR
Plasmid#20686DepositorInsertnicotinic acetylcholine receptors (Chrng Mouse)
UseTagsAMV and pA 50ExpressionBacterialMutationLeucine at 9' position in M2 region changed …PromoterAvailable sinceApril 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENTR FOXP3 WRR
Plasmid#16365DepositorInsertFOXP3 (FOXP3 Human)
UseTagsExpressionBacterialMutationWRR: N361W, E399R, E401RPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pENTR FOXP3 EARR
Plasmid#16367DepositorInsertFOXP3 (FOXP3 Human)
UseTagsExpressionBacterialMutationEARR: N361E, H365A, E399R, E401RPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)
Plasmid#238185PurposeLentiviral vector for overexpression KLF4 ORF from a EF1a promoterDepositorInsertKLF4 (KLF4 Synthetic, Human)
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)
Plasmid#238181PurposeLentiviral vector for overexpression KLF5 ORF from a EF1a promoterDepositorInsertKLF5 (KLF5 Synthetic, Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)
Plasmid#238184PurposeLentiviral vector for overexpression KLF5 ORF from a EF1a promoterDepositorInsertKLF5 (KLF5 Synthetic, Human)
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)
Plasmid#238167PurposeLentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertNon-targeting sgRNA
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)
Plasmid#238182PurposeLentiviral vector for overexpression KLF4 ORF from a EF1a promoterDepositorInsertKLF4 (KLF4 Synthetic, Human)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWF079_LVt-EF1a-TCRGibson_humanTRAC
Plasmid#233448PurposeGibson assembly of TCRB-P2A-TCRaDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SIV_Rev
Plasmid#236244Purpose3rd generation SIV-based lentiviral packaging plasmid; Contains SIV RevDepositorInsertSIV Rev
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKI-P2A-GFP-DOX-N
Plasmid#229407PurposeLentiviral or overpexression of cDNADepositorInsertMKI-P2A-GFP
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTRE3GAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKI-P2A-GFP
Plasmid#229405PurposeLentiviral or overpexression of cDNADepositorInsertMKI-P2A-GFP
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterEFSAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
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AAVS1_dCas9-XTEN-KRAB-p2A-mCherry
Plasmid#222107PurposeDonor vector for CRISPRi integration at AAVS1 with an mCherry fluorophoreDepositorInsertCRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
UseAAV and CRISPRTagsHA and P2A-mCherryExpressionMammalianMutationD10A, H840APromoterAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_dCas9-XTEN-KRAB-p2A-GFP
Plasmid#222108PurposeDonor vector for CRISPRi integration at AAVS1 with a GFP fluorophoreDepositorInsertCRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)
UseAAV and CRISPRTagsHA and P2A-GFPExpressionMammalianMutationD10A, H840APromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 WT
Plasmid#185632Purposeexpression of GST-tagged wild-type PML B-box1 domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationnonePromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 C4C4
Plasmid#185640Purposeexpression of GST-tagged mutant PML B-box1 H155C/H161C domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationH155C H161CPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 W157E
Plasmid#185641Purposeexpression of GST-tagged mutant PML B-box1 W157E domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationW157EPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PML B-box1 F158E
Plasmid#185642Purposeexpression of GST-tagged mutant PML B-box1 F158E domain in bacterial cellsDepositorInsertpromyelocytic leukemia protein (PML Human)
UseTagsGSTExpressionBacterialMutationF158EPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only
