We narrowed to 16,881 results for: SHE

1. pFastBacMam2_N_His8_eGFP_TEV

   Plasmid

   #178806

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   His8, eGFP, TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pet28a_Synthetic_Human_H2A.1

   Plasmid

   #42634

   Depositor

   Joe Landry

   Insert

   Human H2A.1 (H2AC13 Synthetic, Human)
   

   Use

   Tags

   Untagged

   Expression

   Bacterial

   Mutation

   Codon Optimized

   Promoter

   T7

   Available since

   Feb. 25, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pExp-Trx

   Plasmid

   #112577

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Trx (thioredoxin (trxA) from E. coli fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8xHis-TrxA and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT-TODestFLAGHAhTTP

   Plasmid

   #48738

   Purpose

   Expresses hTTP

   Depositor

   Thomas Tuschl

   Insert

   TTP (ZFP36 Human)
   

   Use

   Tags

   FLAG, HA, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, 2x Tet

   Available since

   June 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTBY1-T4uvsY

   Plasmid

   #228214

   Purpose

   Expression in BL21(DE3) of T4 UvsY-like recombination mediator

   Depositor

   Stephen Benkovic

   Insert

   uvsY (uvsY Escherichia phage T4)
   

   Use

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_221

   Plasmid

   #169142

   Purpose

   Constitutive expression of EGFP and sgRNAs targeted to EGFP for measurement of activity of AsCas12a\EnAsCas12a.

   Depositor

   John Doench, David Root

   Insert

   EGFP , EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pExp-MsyB

   Plasmid

   #112570

   Purpose

   Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag

   Depositor

   Marko Hyvönen

   Type

   Empty backbone

   Use

   Tags

   8His and Strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AbVec2.0-mIghg2c

   Plasmid

   #127159

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotype

   Depositor

   Hedda Wardemann

   Insert

   Ighg2c (Ighg2c Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV IE1 gene promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CMV-iRFP713(V256C)-P2A-EGFP

   Plasmid

   #198071

   Purpose

   The AAV vector for iRFP713(V256C) and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP713(V256C)
   

   Use

   AAV

   Tags

   P2A-EGFP

   Expression

   Mutation

   V256C mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGHE-PdNIP2-1

    Plasmid

    #108447

    Purpose

    PdNIP2-1 gene from Date Palm (Phoenix dactylifera) cloned in pGHE. Can be used for Xenopus oocyte assay. The vector has Xenopus globin 3UTR and 5UTR, pT7, and polyA.

    Depositor

    Richard Bélanger

    Insert

    PdNIP2-1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pT7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pExp-mCherry

    Plasmid

    #112569

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-RfxCas13d-Blast-BFP

    Plasmid

    #196726

    Purpose

    Sortable and selectable RfxCas13d.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pExp-Bla

    Plasmid

    #112561

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-Bla and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-link-ymScarletI-SpHis5

    Plasmid

    #219844

    Purpose

    tagging vector to tag genes with ymScarletI and a HIS5 gene

    Depositor

    Bas Teusink

    Insert

    ymScarletI-SpHis5
    

    Use

    Tags

    ymScarletI

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pExp-IF2

    Plasmid

    #112567

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and IF2 (domain I of translation initiation factor IF-2 from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-IF2 and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB CAG-sfGFP-T2A-iCreERT2

    Plasmid

    #177309

    Purpose

    CAG-sfGFP-T2A-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP-T2A-iCreERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-ABE8e-NRCH

    Plasmid

    #165416

    Purpose

    Expresses the base editor ABE8e-NRCH in mammalian cells

    Depositor

    David Liu

    Insert

    ABE8e-NRCH
    

    Use

    Tags

    BP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-STAT6-IRES-Neo

    Plasmid

    #35482

    Depositor

    Axel Nohturfft

    Insert

    STAT6 (STAT6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-Foxo4

    Plasmid

    #17549

    Depositor

    Domenico Accili

    Insert

    FoxO4 (FOXO4 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. nuc-mCherry-pTRIP

    Plasmid

    #163520

    Purpose

    Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

    Depositor

    Thomas Weber

    Insert

    nucleus targeted mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV.TBG.PI.FLPo.rBG

    Plasmid

    #236288

    Purpose

    AAV vector expressing FLPo from TBG promoter

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    FLPo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TBG

    Available since

    May 20, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid

    #177308

    Purpose

    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCAG-N2cL

    Plasmid

    #100812

    Purpose

    express rabies virus CVS N2c strain polymerase gene (N2cL)

    Depositor

    Ian Wickersham

    Insert

    rabies virus CVS N2c strain polymerase gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-N2cN

    Plasmid

    #100801

    Purpose

    express rabies virus CVS N2c strain nucleoprotein gene (N2cN)

    Depositor

    Ian Wickersham

    Insert

    rabies virus CVS N2c strain nucleoprotein gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-N2cP

    Plasmid

    #100808

    Purpose

    express rabies virus CVS N2c strain phosphoprotein gene (N2cP)

    Depositor

    Ian Wickersham

    Insert

    rabies virus CVS N2c strain phosphoprotein gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. tet-pLKO.puro_shGFP

    Plasmid

    #110421

    Purpose

    shGFP control, silence GFP gene, doxycycline inducible, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO (RNA PolIII)

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. RABEP1A-c019

    Plasmid

    #175477

    Purpose

    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    RABEP1 (RABEP1 Human)
    

    Use

    Tags

    TEV-TwinStrep

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET32a-Trx-His6-TEV_SacB

    Plasmid

    #164588

    Purpose

    Bacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.

    Depositor

    Ernst Schonbrunn

    Insert

    TEV-SacB
    

    Use

    Tags

    Trx-His6 followed by TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEGFP-centrin-1

    Plasmid

    #72641

    Purpose

    The plasmid encodes centrin-1 with an N-terminal EGFP tag

    Depositor

    Michel Bornens

    Insert

    centrin-1 (CETN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJA54 HLA-A*03:01

    Plasmid

    #180451

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*03:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCDNA_Lifeact-GFP_NLS-mCherry

    Plasmid

    #69058

    Purpose

    Dual Fluorescent Reporter for F-actin and Nuclei

    Depositor

    Olivier Pertz

    Insert

    LifeAct-GFP_IRES_NLS-mCherry
    

    Use

    Tags

    Lifeact-GFP, NLS-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-syn-DIO-axon-jGCaMP8f

    Plasmid

    #220531

    Purpose

    For Cre-dependent enriched expression of GCaMP8f in axons

    Depositor

    Lin Tian

    Insert

    Axon-DIO-GCaMP8f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AbVec2.0-mIghg3

    Plasmid

    #127160

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg3 (Ighg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSV40-mCherry-P2A-Hygro

    Plasmid

    #106478

    Purpose

    Expression of mCherry and Hygromycin resistance gene from SV40 promoter

    Depositor

    Alex Hewitt

    Inserts

    mCherry
    Hygromycin B resistance
    

    Use

    Tags

    Expression

    Mammalian and Plant

    Mutation

    Promoter

    SV40 promoter

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBABE.is4-mOpn

    Plasmid

    #74410

    Purpose

    Expresses mouse Osteopontin from transcript tv4 in mammalian cells

    Depositor

    Georgios Stathopoulos

    Insert

    mouse Opn (Spp1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pT2/SV-Venus-SIINFEKL

    Plasmid

    #111624

    Purpose

    Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies

    Depositor

    Jon Yewdell

    Insert

    Venus-LEQLE-SIINFEKL-TEW
    

    Use

    Tags

    LEQLE-SIINFEKL-TEW

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. AbVec_IGHC

    Plasmid

    #183701

    Purpose

    Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGHG1 (IGHG1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AbVec_IGKC

    Plasmid

    #183702

    Purpose

    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGKC (IGKC )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCEV-G2-Km ymNeongreen

    Plasmid

    #193959

    Purpose

    TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast

    Depositor

    Bas Teusink

    Insert

    ymNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AbVec_IGLC

    Plasmid

    #183703

    Purpose

    Encode human Ig lambda chain constant region. For cloning variable region and mAb expression.

    Depositor

    David Nemazee

    Insert

    IGLC2 (IGLC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLIX403-ccdB-G418

    Plasmid

    #158561

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pLIX403-EYFP-ccdB-Hygro

    Plasmid

    #158555

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pTCF158 Human beta-2 microglobulin

    Plasmid

    #180457

    Purpose

    E. coli expression of class I MHC light chain

    Depositor

    John Altman

    Insert

    Human beta-2 microglobulin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCIG3 Rab5CA

    Plasmid

    #78268

    Purpose

    Rab5 Constitutively active (Q79L) expression

    Depositor

    Felicia Goodrum

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    CMV, Lentiviral LTR

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pTCF236 HLA-B*40:01

    Plasmid

    #180463

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*40:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    Feb. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pTCF7 HLA-B*07:02

    Plasmid

    #180446

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-B*07:02
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pExp-sfGFP

    Plasmid

    #112573

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge