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pCAGGS-chGrb2/eDHFR(69K6)-iRFP713

Plasmid

#178854

Purpose

Mammalian expression of Grb2-eDHFR(69K6) chimera fused to iRFP713

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

Grb2(1-59)-eDHFR(69K6)-Grb2(152-216)-iRFP713 (GRB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Grb2: Y160E

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP670-eDHFR(69K6)-G-alpha-s

Plasmid

#172105

Purpose

Mammalian expression of Gαs fused to miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)-Gαs (GNAS Human)

Use

Tags

Expression

Mammalian

Mutation

Gαs: C3S

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-Stx3 L165A/E166A-2xMyc/His

Plasmid

#99743

Purpose

Expresses human Stx3 locked into the open conformation with a C-term myc-myc-his tag in mammalian cells.

Depositor

Thomas Weimbs

Article

Giovannone et al Mol Biol Cell. 2017 Oct 15;28(21):2843-2

Insert

Stx3 (STX3 Human)

Use

Tags

myc-myc-his

Expression

Mammalian

Mutation

Changed both L165 and E166 to Alanine

Promoter

CMV

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2462

Plasmid

#97064

Purpose

Expressing Sulfolobus solfataricus orf SSO2462 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2462

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2452

Plasmid

#97059

Purpose

Expressing Sulfolobus solfataricus orf SSO2452 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2452

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF126

Plasmid

#97021

Purpose

Expressing Pyrobaculum spherical virus orf126 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 126

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDEHISSIRV119

Plasmid

#97054

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 119 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 119

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDEHISSIRV55

Plasmid

#97055

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 55 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 55

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA5006

Plasmid

#97016

Purpose

Expressing Pseudomonas aeruginosa orf PA5006 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P aeruginosa orf PA5006

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEANHISECArdB

Plasmid

#96967

Purpose

Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

E. coli ArdB

Use

Tags

N-ter TEV cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA0856

Plasmid

#97014

Purpose

Expressing Pseudomonas aeruginosa orf PA0856 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P aeruginosa orf PA0856

Use

Tags

N-ter TEV cleavable 6HIs

Expression

Mutation

N-terminal deletion (AA 1-34)

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA4631

Plasmid

#97017

Purpose

Expressing Pseudomonas aeruginosa orf PA4631 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Nucleoside-diphosphate-sugar epimerase

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISSSO2373

Plasmid

#97058

Purpose

Expressing Sulfolobus solfataricus orf SSO2373 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2373

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISPApqsL

Plasmid

#97020

Purpose

Expressing Pseudomonas aeruginosa pqsL protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative FAD-dependent monooxygenase

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAPPFK

Plasmid

#97003

Purpose

Expressing MRSA PPFK protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative phosphofructokinase

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISSO1404

Plasmid

#97056

Purpose

Expressing Sulfolobus solfataricus orf SSO1404 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO1404

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISPSVORF239

Plasmid

#97025

Purpose

Expressing Pyrobaculum spherical virus orf239 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 239

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISPSVORF165A

Plasmid

#97024

Purpose

Expressing Pyrobaculum spherical virus orf165a protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 165a

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISPSVORF137

Plasmid

#97023

Purpose

Expressing Pyrobaculum spherical virus orf137 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 137

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEAHISMRSA1376

Plasmid

#97002

Purpose

Expressing MRSA sar1376 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative 4-oxalocrotonate tautomerase

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge