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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,198 results for: e.coli

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Showing: 401 - 450 of 1198 results

  12. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Article
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86846-plasmid-map-sequence-id-172845

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  14. pAD-GamS

    Plasmid
    #99246
    Purpose
    Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.
    Depositor
    Jeff Hasty
    Article
    Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102
    Insert
    gamS (with C-terminal 6xHis tag)
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T7lac
    Available Since
    Oct. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    99246-plasmid-map-sequence-id-339990

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  15. pIRE4-Bpa

    Plasmid
    #155342
    Purpose
    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.
    Depositor
    Irene Coin
    Article
    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15
    Insert
    humanized EBpaRS/BstYam
    Expression
    Mammalian
    Promoter
    PGK/U6
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155342-plasmid-map-sequence-id-310519

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  19. pBUN279

    Plasmid
    #60674
    Purpose
    integration of M. smegmatis ald gene in Mycobacterium using mycobacteriophage L5 attB site
    Depositor
    Raul Barletta
    Article
    Feng et al J Bacteriol. 2002 Sep;184(18):5001-10.
    Insert
    ald
    Use
    E.coli-mycobacterium integration-proficient vector
    Available Since
    July 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. ARK2aa

    Plasmid
    #81013
    Purpose
    E.coli BW25113 + JBEI-12050 (pBbA5c-MevTo-BBa1002-pTrc-Mkco-aphA, JBx_049165 ) + JBEI-9314 (pTrc99a-PMDsc, JBx_039366)
    Depositor
    Taek Soon Lee
    Article
    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10
    Inserts
    MevTo-BBa1002-pTrc-Mkco-aphA
    and PMD
    Promoter
    LacUV5 and Trc promoter
    Available Since
    Aug. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    81013-plasmid-map-sequence-id-153394

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  27. pET32-sLatherin

    Plasmid
    #61685
    Purpose
    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion
    Depositor
    Brian Smith
    Article
    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20
    Insert
    Latherin (LATH horse)
    Tags
    HIS and Trx-Tag thioredoxin protein
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  28. cr3Ec

    Plasmid
    #184860
    Purpose
    cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system
    Depositor
    Patrick Sobetzko
    Article
    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:
    Insert
    Cas9, gam, bet, exo
    Tags
    Cas9 ssrA degradation tag
    Expression
    Bacterial
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    184860-plasmid-map-sequence-id-363182

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  36. pHL 1454

    Plasmid
    #53019
    Purpose
    AmpR + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Insert
    fimB
    Use
    Synthetic Biology
    Tags
    RBS(st7)
    Expression
    Bacterial
    Promoter
    pLlac0-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 401 - 450 of 1198 results