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Showing: 401 - 450 of 848 results

  1. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113699
    Purpose
    Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113699-plasmid-map-sequence-id-229551

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  2. pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct

    Plasmid
    #156452
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTG
    Depositor
    Elphege Nora
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    RAD21, AID (Rad21 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156452-plasmid-map-sequence-id-310171

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  3. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-G60A)

    Plasmid
    #176144
    Purpose
    EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Gly60 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176144-plasmid-map-sequence-id-353152

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  4. pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-R65A)

    Plasmid
    #176143
    Purpose
    EGFP fused the N-terminus of POLB, linked by T2A to N-terminus Myc-tagged SIRT6 with the mutation Arg65Ala, a mutation in the PAM site used by SIRT6-KO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    PolB-T2A-SIRT6 (POLB Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Mutation in Arg65 to Ala, and a mutation in the P…
    Promoter
    EF1A
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176143-plasmid-map-sequence-id-353151

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  5. pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

    Plasmid
    #156433
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    SORORIN, AID (Cdca5 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156433-plasmid-map-sequence-id-310157

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  7. pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

    Plasmid
    #175549
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.
    Depositor
    Elzo de Wit
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    WAPL (Wapl Mouse)
    Use
    Mouse Targeting
    Tags
    AID[71-114]-eGFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175549-plasmid-map-sequence-id-345088

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  8. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid
    #176141
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutation in Asp256 to Ala, a…
    Promoter
    EF1A
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176141-plasmid-map-sequence-id-353041

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  9. 3` CC: Hygro – GFP-N – loxP – mScarlet

    Plasmid
    #219564
    Purpose
    3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    3` CC: Hygro – GFP-N – loxP – mScarlet
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    EF1-a
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219564-plasmid-map-sequence-id-438335

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  12. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131461
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131461-plasmid-map-sequence-id-305184

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  13. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

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  14. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131463
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A G34R, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available since
    July 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131463-plasmid-map-sequence-id-305001

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  15. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid
    #67277
    Purpose
    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.
    Depositor
    Jannik Elverløv-Jakobsen
    Article
    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do
    Inserts
    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    Use
    Tags
    mTQ2 blue fluorescent and red flourescent gene ka…
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67277-plasmid-map-sequence-id-121189

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  27. pJS145

    Plasmid
    #179159
    Purpose
    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.
    Depositor
    Iva Greenwald
    Article
    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11
    Insert
    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    Use
    Tags
    Expression
    Worm
    Mutation
    Flexon replacing 2nd intron of 2xNLS::GFP
    Promoter
    rps-27p
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    179159-plasmid-map-sequence-id-352801

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  28. pFA0057

    Plasmid
    #131784
    Purpose
    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.
    Depositor
    Frank Albert
    Article
    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.
    Insert
    20mer GFP cassette guide (gGFP) and 5' sgRNA
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131784-plasmid-map-sequence-id-258729

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  29. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

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  34. pZBTB5-donor

    Plasmid
    #112303
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZBTB5 with GFP
    Depositor
    Kevin White
    Insert
    ZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112303-plasmid-map-sequence-id-228415

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  35. pZFX-donor

    Plasmid
    #112366
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFX with GFP
    Depositor
    Kevin White
    Insert
    ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112366-plasmid-map-sequence-id-229714

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  36. pZSCAN18-donor

    Plasmid
    #113821
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZSCAN18 with GFP
    Depositor
    Kevin White
    Insert
    ZSCAN18 homology arms flanking EGFP-IRES-Neo cassette (ZSCAN18 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    113821-plasmid-map-sequence-id-241248

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  37. pZNF561-donor

    Plasmid
    #113801
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF561 with GFP
    Depositor
    Kevin White
    Insert
    ZNF561 homology arms flanking EGFP-IRES-Neo cassette (ZNF561 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113801-plasmid-map-sequence-id-229885

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  38. pZNF700-donor

    Plasmid
    #112385
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF700 with GFP
    Depositor
    Kevin White
    Insert
    ZNF700 homology arms flanking EGFP-IRES-Neo cassette (ZNF700 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112385-plasmid-map-sequence-id-229845

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  39. pZNF169-donor

    Plasmid
    #112376
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF169 with GFP
    Depositor
    Kevin White
    Insert
    ZNF169 homology arms flanking EGFP-IRES-Neo cassette (ZNF169 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112376-plasmid-map-sequence-id-229740

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  40. pZFP1-donor

    Plasmid
    #112374
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFP1 with GFP
    Depositor
    Kevin White
    Insert
    ZFP1 homology arms flanking EGFP-IRES-Neo cassette (ZFP1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112374-plasmid-map-sequence-id-229736

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  41. pZNF227-donor

    Plasmid
    #112368
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF227 with GFP
    Depositor
    Kevin White
    Insert
    ZNF227 homology arms flanking EGFP-IRES-Neo cassette (ZNF227 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112368-plasmid-map-sequence-id-229716

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  42. pOTX1-donor

    Plasmid
    #112364
    Purpose
    CRISPR donor plasmid to tag human transcription factor OTX1 with GFP
    Depositor
    Kevin White
    Insert
    OTX1 homology arms flanking EGFP-IRES-Neo cassette (OTX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112364-plasmid-map-sequence-id-229691

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  43. pZNF175-donor

    Plasmid
    #112347
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF175 with GFP
    Depositor
    Kevin White
    Insert
    ZNF175 homology arms flanking EGFP-IRES-Neo cassette (ZNF175 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112347-plasmid-map-sequence-id-229660

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  44. pZNF354C-donor

    Plasmid
    #112339
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF354C with GFP
    Depositor
    Kevin White
    Insert
    ZNF354C homology arms flanking EGFP-IRES-Neo cassette (ZNF354C Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112339-plasmid-map-sequence-id-228576

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  45. pZNF347-donor

    Plasmid
    #112326
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF347 with GFP
    Depositor
    Kevin White
    Insert
    ZNF347 homology arms flanking EGFP-IRES-Neo cassette (ZNF347 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112326-plasmid-map-sequence-id-228548

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  49. pFnCpf1GG

    Plasmid
    #131010
    Purpose
    Backbone plasmid for generating CRISPR arrays for FnCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of FnCas12a
    GFP expression cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131010-plasmid-map-sequence-id-257701

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  50. pAsCpf1GG

    Plasmid
    #131011
    Purpose
    Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of AsCas12a
    GFP expression cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131011-plasmid-map-sequence-id-258106

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Showing: 401 - 450 of 848 results