Addgene

We narrowed to 12,549 results for: LIC

Showing: 4451 - 4500 of 12549 results

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p404-Grx5-GFP1-10

Plasmid

#91952

Purpose

Expresses GFP1-10 in the mitochondrial matrix

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

Grx5-GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pQTEV-RAB17

Plasmid

#34812

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

RAB17 (RAB17 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin B2

Plasmid

#10911

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin B2 (CCNB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pDX_init containing Sb-loop

Plasmid

#132697

Purpose

pDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Sb_loop

Use

M13 phagmid vector

Tags

Expression

Mutation

Promoter

lac

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pNCS Citrine

Plasmid

#91761

Purpose

Bacterial expression of a fluorescent protein, Citrine

Depositor

Erik Rodriguez, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Insert

Citrine

Use

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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mTFP1-Lifeact-7

Plasmid

#54749

Purpose

Localization: Actin, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pWS5392-CRISPRai-HIS3

Plasmid

#182831

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - HIS3 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5393-CRISPRai-TRP1

Plasmid

#182832

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - TRP1 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5395-CRISPRai-LYS2

Plasmid

#182834

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - LYS2 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5399-CRISPRai-ZeoR

Plasmid

#182838

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS5391-CRISPRai-LEU2

Plasmid

#182830

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - LEU2 marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti MS2-p65-HSF1 sfGFP

Plasmid

#171051

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

MS2-p65-HSF1_sfGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-PA(WT)-Vav2

Plasmid

#86977

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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p5E-CMVmin

Plasmid

#80802

Purpose

minimal cytomegalovirus immediate early enhancer/promoter (CMVmin) from pcDNA3

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

minimal cytomegalovirus immediate early enhancer/promoter

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pQTEV-TAGLN2

Plasmid

#31569

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

transgelin 2 (TAGLN2 Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pGL2_171

Plasmid

#199103

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pWKS1 ORI plus NGS barcode 17, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pNB778

Plasmid

#60154

Purpose

Green click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Use

Tags

Expression

Yeast

Mutation

Promoter

LEU1pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin D1

Plasmid

#8962

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D1 (CCND1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
EZ-L439

Plasmid

#169146

Purpose

Encodes OptoINVRT7 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

Insert

VP16-EL222

Use

Tags

SV40 NLS

Expression

Yeast

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427V

Plasmid

#81044

Purpose

mammalian expression of LOV2 I427V

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427V

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427V

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
R-G324D-(Kin-8)

Plasmid

#28179

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Use

Tags

Expression

Mammalian

Mutation

G324D

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pTopo pLox hPGK-Puro pA pLox

Plasmid

#171048

Purpose

Empty donor template for CRISPR/Cas9 targeting of TERT RE's

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriEx-mCherry-LOV2 V416I

Plasmid

#81045

Purpose

mammalian expression of LOV V416I

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV V416I

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416I

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEpic

Plasmid

#84372

Purpose

3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
SGL40C.EFS.E2Crimson

Plasmid

#100894

Purpose

Lentiviral gRNA delivery

Depositor

Dirk Heckl

Article

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Insert

E2Crimson

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-ReDNAP (Y427A)

Plasmid

#52346

Purpose

CEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

Recoded TP-DNAP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

REV1

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pQTEV-PEPD

Plasmid

#31328

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

Insert

peptidase D (PEPD Human)

Use

Tags

His and TEV

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4TO OM-eLIR

Plasmid

#189004

Purpose

mitophagy-inducing adaptor protein

Depositor

Atsushi Hoshino

Article

Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

Insert

OM-eLIR

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_PflaB-iRFPBb

Plasmid

#118237

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven iRFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven iRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL12-GCaMP6f-pHuji bb118

Plasmid

#158981

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to pHuji fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-pHuji

Use

Tags

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833 a…

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMrfp

Plasmid

#59773

Purpose

Derived from pCM62; ampR, tetR, PBAD - rfp

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-XI2

Plasmid

#176156

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Hp MTAN

Plasmid

#69802

Purpose

Expression of H. pylori J99 methylthioadenosine nucleosidase

Depositor

Vern Schramm

Article

Wang et al Biochemistry. 2012 Sep 4;51(35):6892-4.

Insert

Methylthioadenosine nucleosidase

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pACBB-eGFP

Plasmid

#32551

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Mutation

Promoter

Consitutive lac

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pET19b_4CL + STS

Plasmid

#139790

Purpose

expression of biosynthetic enzymes 4CL and STS

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL
STS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-uniRapR-P-Vav2(DPZ)

Plasmid

#91875

Purpose

Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Insert

Vav2 (VAV2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MCV-HF

Plasmid

#32057

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-HF genome

Use

Tags

Expression

Mutation

Promoter

none from vector. Insert contains native promoter…

Available since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-P2A-CFP no-pA

Plasmid

#80827

Purpose

P2A followed by CFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-CFP no pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTR1-GPD-TRPC

Plasmid

#190103

Purpose

Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection

Depositor

Perng-Kuang Chang

Article

Chang et al Appl Environ Microbiol. 2012 Nov;78(21):

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFZifPZ431

Plasmid

#185849

Purpose

Expressing artificial trans-activator in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Article

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>ZifPZ43>MED3>PABF1

Use

Tags

Expression

Yeast

Mutation

None

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCoofy50

Plasmid

#55189

Purpose

Mammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC.

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Use

Tags

8x His, FLAG, HA, PreScission site (3C protease s…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKL13-MgtC bb118

Plasmid

#158982

Purpose

Constitutive expression (sigma 70 promoter) of mgtC protein from S. Typhimurium in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

mgtC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Bba_J23118 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8379

Plasmid

#126913

Purpose

Plasmid containing integration cassette at X-4 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and XdCrtYB

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB8381

Plasmid

#126915

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and tHMG1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS MCK CRE

Plasmid

#12529

Depositor

Ronald Kahn

Article

Bruning et al Mol Cell. 1998 Nov . 2(5):559-69.

Insert

Cre recombinase

Use

Cre/Lox; Transgenic construct

Tags

Expression

Mutation

Cre is driven by the Muscle Creatine Kinase promo…

Promoter

Available since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMUT2-pCaf-mCherry

Plasmid

#192860

Purpose

pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Nanobody-CadC fusion protein and cognate promoter inducible by caffeine

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Myo 9b

Plasmid

#134907

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Reinhard et al EMBO J. 1995 Feb 15;14(4):697-704.

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PTEN-mut (negative control)

Plasmid

#86646

Purpose

expression of control shRNA

Depositor

Jaime Modiano

Article

Lin et al Mol Cancer. 2007 Feb 14;6:16.

Insert

PTEN (control)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

June 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 HA Sam68 G178E

Plasmid

#17689

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

G178E

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pKM061

Plasmid

#134174

Purpose

Bacterial expression of GST-HJURP

Depositor

Iain Cheeseman

Article

McKinley et al Cell. 2014 Jul 17;158(2):397-411. doi: 1

Insert

HJURP (HJURP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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