We narrowed to 13,271 results for: cDNA

1. ODD-Luciferase-pcDNA3

   Plasmid

   #18965

   Depositor

   William Kaelin

   Article

   Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

   Insert

   ODD-Firefly Luciferase (HIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HIF1a ODD sequence fused in frame to luciferase O…

   Promoter

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)GαoA-91-NLuc

   Plasmid

   #207151

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant A of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Promoter

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)GαoB-91-NLuc

   Plasmid

   #207152

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes transcript variant B of GNAO tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Dominique Schols, Tom Van Loy

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)G

   Promoter

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-ccdB-3xFLAG

   Plasmid

   #214984

   Purpose

   Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag

   Depositor

   Wade Harper

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1

   Plasmid

   #159130

   Purpose

   expresses BACH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   BACH1 (BACH1 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-DN-hCUL3-FLAG

   Plasmid

   #15820

   Depositor

   Wade Harper

   Article

   Jin et al Methods Enzymol. 2005;399:287-309. doi:

   Insert

   Cullin3 (CUL3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Dominant negative: N418

   Promoter

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. RelA cFlag pcDNA3

   Plasmid

   #20012

   Depositor

   Stephen Smale

   Article

   Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

   Insert

   RelA (Rela Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Akt-STOPS

   Plasmid

   #175006

   Purpose

   Akt Substrate-based Tandem Occupancy Peptide Sponge. Genetically encoded for perturbing cellular Akt kinase activity.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-STOPS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/VChR1-EYFP

   Plasmid

   #20955

   Purpose

   Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

   Insert

   volvox channelrhodopsin-1
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-hTICAM1-VenusFlag

    Plasmid

    #197632

    Purpose

    Human TRIF protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    TICAM1 (TICAM1 Human)
    

    Use

    Tags

    Venus-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3/Myc-DNMT3A

    Plasmid

    #35521

    Purpose

    Mammalian expression of human DNMT3A with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3A (DNMT3A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-PM-ExRai-AktAR2

    Plasmid

    #184050

    Purpose

    Plasma membrane-targeted ExRai-AktAR2

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    PM-ExRai-AktAR2
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-erExRai-AKAR2

    Plasmid

    #161756

    Purpose

    ExRai-AKAR2 targeted to the cytosolic face of the ER membrane.

    Depositor

    Jin Zhang

    Article

    Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

    Insert

    CYP450-ExRai-AKAR2
    

    Use

    Tags

    N-terminal 27 amino acids from CYP450

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF151 pcDNA3.1(+)SOD1WT

    Plasmid

    #26397

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-3xFLAG-V5-ccdB

    Plasmid

    #87064

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1436 pcDNA3 Flag HA

    Plasmid

    #10792

    Purpose

    pcDNA3 vector with added Flag, HA tags

    Depositor

    William Sellers

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-Dio-GFP

    Plasmid

    #198279

    Purpose

    Cre reporter for mammalian cells; double-floxxed-EGFP

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Dio-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-FluoSTEP-AKAR

    Plasmid

    #181844

    Purpose

    Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-AKAR
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDNA-3xFLAG-Pontin

    Plasmid

    #51635

    Purpose

    Mammalian expression of 3xFLAG tagged pontin, N-terminal.

    Depositor

    Steven Artandi

    Article

    Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

    Insert

    Pontin (RUVBL1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-RAB-EKARev

    Plasmid

    #118474

    Purpose

    ddRFP-based single-color biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-EKARev
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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