We narrowed to 59,825 results for: Gene
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Plasmid#102916Purposeexpresses ABE6.3 in mammalian cellsDepositorInsertABE6.3
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-4
Plasmid#72679PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC74
Plasmid#62342PurposesgRNA + 1x COM with COM-KRAB effector for mammalian cellsDepositorInsertssgRNA + 1x COM binding module
COM-KRAB
UseLentiviralTagsKRABExpressionMammalianMutationTargets sv40 promoter, sequence: GAATAGCTCAGAGGCC…PromoterCMV and U6Available SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYZ1389
Plasmid#215348PurposeNla CRISPRi Delivery VectorDepositorInsertNla CRISPRi Genes (Cas5, Cas8c, Cas7)
UseCRISPR; Nla i delivery vectorAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-TT'
Plasmid#37823DepositorInsertPromotorless gfp reporter gene
Available SinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ExRai-AMPKAR
Plasmid#192446PurposeGenetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.DepositorInsertExRai-AMPKAR
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tagExpressionMammalianPromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-oChIEF-tdTomato
Plasmid#50977PurposeAAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITRDepositorInsertChIEF-tdTomato
UseAAVTagstdTomatoMutationChIEF gene is mammalian codon optimizedPromoterhuman synapsin promoterAvailable SinceFeb. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Fos-CreERT2
Plasmid#194643PurposeExpresses Cre-ERT2 under the control of c-Fos promoterDepositorInsertFos-CreERT2
UseAAVExpressionMammalianAvailable SinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Nopt
Plasmid#231704PurposeMammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.DepositorInsertVSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)
UseVsv expressionExpressionMammalianMutationhuman codon-optimizedPromoterCMVAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
TagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…Available SinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Syn-ATP
Plasmid#51819PurposeOptical reporter of presynaptic ATPDepositorInsertChimeric Synaptophysin-mCherry-Luciferase
ExpressionMammalianMutationWithin WT luciferase gene, changed Threonine 214 …PromoterCMVAvailable SinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX-GT
Plasmid#26198DepositorInsertEnhanced green fluorescent protein, TVA (eGFP Mouse, TVA is a mouse codon optimized version of an avian gene.)
UseAAV and Cre/LoxMutationTVA mammalian codon optimizedAvailable SinceSept. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
P4 cosmid
Plasmid#196335PurposeP4 cosmid with CRISPR-Cas9 systemDepositorInsertgenes from phage P4, CRISPR-Cas9 system
UseCRISPRExpressionBacterialAvailable SinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pG108-K - AhpC-Glow
Plasmid#185135PurposeBacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers BS2 is cloned under the Bacteroides thetaiotaomicron ahpC promoterDepositorInsertsGlow gene
Bacteroides thetaiotaomicron ahpC promoter
UsePg106ExpressionBacterialAvailable SinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-fFuCas9-HTBNLS-hph
Plasmid#121092PurposeMultigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 systemDepositorInsertCas9 and hygromycin resist gene
UseCRISPRExpressionBacterialPromotergpdA;trpCAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
Adeno Cas9
Plasmid#64072PurposeAdenoviral vector for the expression of the Cas9DepositorTypeEmpty backboneUseAdenoviralTagsFLAGPromoterU6 and CBhAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKCcas9dO
Plasmid#62552PurposeHigh efficiency Streptomyces genome editing by CRISPR/Cas9 systemDepositorInsertsScocas9
sgRNA ()
act-orf4 homology arm
UseCRISPR; Bacterial genome editingExpressionBacterialPromoterj23119 and ptipAAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRN11
Plasmid#84455Purposeshuttle plasmid for sarA P1-mCherry expressionDepositorInsertmCherry
UseShuttle vector e.coli-s.aureus, expression of sgf…Available SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIVTRup
Plasmid#101362PurposeHighly efficient in vitro transcription. Empty backbone.DepositorInsert5'UTR-EcoRV site-3'UTR-pAtrack(55 pA)
UseFor cloning a gene for in vitro transcription pur…PromoterT7Available SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Basic-hCYP26A1P-E4-luciferase
Plasmid#135592PurposeShort form DNA fragment of human CYP26A1gene promoter (E4) in pGL3-Basic-luc vectorDepositorInsertShort form promoter of human CYP26A1 gene (E4)
PromoterHuman CYP26A1 promoter (Short Form, E4)Available SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev
Plasmid#118474PurposeddRFP-based single-color biosensor for monitoring ERK activity.DepositorInsertRAB-EKARev
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable SinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.8
Plasmid#102917Purposeexpresses ABE7.8 in mammalian cellsDepositorInsertABE7.8
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
YE1-BE4max-NG
Plasmid#138159PurposeC-to-T base editorDepositorInsertrAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
TagsNLSExpressionMammalianMutationWithin rAPOBEC1 W90Y + R126E; within Cas9 D10A + …PromoterCMVAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZa pBAD csgA CBDcex pLac Ara csgGEF
Plasmid#226378PurposePlasmid carries csgA and csgG,E,F genes of curli operonDepositorInsertcsgA and csgG,E,F genes of curli operon
ExpressionBacterialPromoterpBADAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iChR2++
Plasmid#98172Purposeartificial anion conducting channelrhodopsin (aACR). Activation with blue light. Codon optimized for mammalian expression.DepositorInsertSynthetic construct iChR2++ gene
TagsmCherryExpressionMammalianMutationA59S, E83N, E90Q, E101S, Q117R, E123S, T159C, V24…PromoterCMV (+enhancer)Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSIE1
Plasmid#136355PurposeSelection by Inducible EffectorsDepositorInsertsType VI effector bfe1
Type VI effector bte1
BT_4676
tetR
UseUnspecifiedMutationATG removed to add signal sequence before genePromoterP1TDP-GHO23Available SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFC333
Plasmid#87844PurposeAMA1 plasmid with Aspergillus optimized Cas9 and ble selection markerDepositorInsertsCas9
ble (bleomycin resistance marker)
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialPromoterAspergillus nidulans tef1 promoter and Aspergillu…Available SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo
Plasmid#128455Purposelentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance geneDepositorInsertEf1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR
Plasmid#31222DepositorInsertCD4-tdTom (CD4 Aequorea victoria, Human, Fly)
TagsCD4 and tdTomatoExpressionInsectMutationFusion of signal peptide of Drosophila Akh gene, …Available SinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only