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pDest-624 (attR4-attR2)

Plasmid

#161879

Purpose

Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R5R4-pHygTK

Plasmid

#141059

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

HygTK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R5R4-pNeo

Plasmid

#141058

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R5R4-pPuroTK

Plasmid

#141060

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

PuroTK

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-S4-UD-mCherr

Plasmid

#140307

Purpose

Expresses Flag-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

contains only the SH4 and UD regions fused to mCh…

Promoter

CMV

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XHA-Src-Y530EEI

Plasmid

#140322

Purpose

Expresses HA-tagged human Src-Y530EEI in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X HA

Expression

Mammalian

Mutation

Y530EEI

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S32

Plasmid

#140309

Purpose

Expresses Flag-tagged human Src-S32 in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

contains only the SH3 and SH2 domains

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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p1146-pCSDest-AcrIIC3Nme-mCherry

Plasmid

#126202

Purpose

Mammalian expression of AcrIIC3Nme fused to mCherry

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

codon-optimized AcrIIC3Nme

Use

Tags

FLAG/NLS and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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JK305: pMVP/Blast-DEST

Plasmid

#121855

Purpose

pMVP destination vector, empty expression vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JL104: pMVP/Neo-DEST

Plasmid

#121857

Purpose

pMVP destination vector, empty expression vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Pmvp gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCWX-R4DESTR2-PGK-Tk:Sh

Plasmid

#114321

Purpose

Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Zeocin/Bleomycin resistance and Acyclovir conditional ablation

Depositor

Patrick Salmon

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARF3-V5-His6

Plasmid

#67412

Purpose

Expression of hArf3 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF4-V5-His6

Plasmid

#67413

Purpose

Expression of hArf4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF5-V5-His6

Plasmid

#67414

Purpose

Expression of hArf5 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL1-V5-His6

Plasmid

#67416

Purpose

Expression of hArl1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL2-V5-His6

Plasmid

#67417

Purpose

Expression of hArl2 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL5A-V5-His6

Plasmid

#67420

Purpose

Expression of hArl5 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

bp 70 C to T; silent mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL8A-V5-His6

Plasmid

#67424

Purpose

Expression of hArl8 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4D-V5-His6

Plasmid

#67426

Purpose

Expression of hArl4D with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL14-V5-His6

Plasmid

#67427

Purpose

Expression of hArl14 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

bp 172 C to T; aa L58F

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only