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Showing: 2821 - 2850 of 167150 results

  9. pAG Lenti CMV Empty Puro

    Plasmid
    #236083
    Purpose
    Empty lentiviral backbone
    Depositor
    Melina Fan
    Type
    Empty backbone
    Use
    Lentiviral
    Promoter
    CMV
    Available Since
    April 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236083-plasmid-map-sequence-id-467823

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  10. PC1575

    Plasmid
    #232088
    Purpose
    Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.
    Depositor
    Pranam Chatterjee
    Article
    Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi
    Insert
    uAb with cloning Esp3I restriction site
    Tags
    IRES-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232088-plasmid-map-sequence-id-464073

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  18. pPolI_SARS-CoV-2_NLuc

    Plasmid
    #183953
    Purpose
    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter
    Depositor
    AJ te Velthuis
    Article
    Vial et al bioRxiv 2021.06.28.450211
    Insert
    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    Expression
    Mammalian
    Promoter
    Mammalian Pol I
    Available Since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    183953-plasmid-map-sequence-id-365335

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  23. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

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  24. pET30a-His6-LOXCAT

    Plasmid
    #140084
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140084-plasmid-map-sequence-id-311164

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  26. lenti sgRNA(MS2)_zeo backbone

    Plasmid
    #61427
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    61427-plasmid-map-sequence-id-322387

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  27. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

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  29. pCMV-15F11-HA-Halo

    Plasmid
    #129592
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the HaloTag. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-HaloTag (15F11-HA scFv-HaloTag)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129592-plasmid-map-sequence-id-254268

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Showing: 2821 - 2850 of 167150 results