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Plasmid#176203PurposeDouble INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization systemDepositorInsertINBox-mCherry-INBox-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG.mCherry-INBox(AAA)-eDHFR
Plasmid#176207PurposeINBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization systemDepositorInsertmCherry-INBoxAAA-eDHFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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SAE2-mGarnet
Plasmid#175240PurposeBacterial expression and purification, combined with SAE1 to form the E1 enzyme heterodimerDepositorInsertUBA2 (UBA2 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FKBP-EGFP-RanGAP
Plasmid#175236PurposeBacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycinDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-RanGAP
Plasmid#175235PurposeBacterial expression and purification, high affinity SUMOylation substrateDepositorInsertRANGAP1 (RANGAP1 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH191
Plasmid#172149PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*, Y184*); [strong promoter eG, strong RB…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH190
Plasmid#172148PurposeAccessory Plasmid for PACE of pylBCD variantsDepositorInsertPpsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT
UseTagsExpressionBacterialMutationgIII(P29*); [strong promoter eG, strong RBS SD8] …PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAraQCas-BsaI
Plasmid#170633PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD/His-D-iLight
Plasmid#170270PurposeExpression of iLight with 6xHis N-terminal tag in bacteria.DepositorInsertiLight
UseTags6xHis tagExpressionBacterialMutationSee CommentsPromoteraraBADAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27Linear-2, PolyU(0)
Plasmid#166994PurposeTests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1, PolyU(0)
Plasmid#166992PurposeTests for the impact of a synthetic linear tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination module:PolyU(0)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(1)
Plasmid#166988PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLNYtRNA promoter.DepositorInsertTermination module:PolyU(1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLY, Linear-3, PolyU(4)
Plasmid#166989PurposeTests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.DepositorInsertTermination module:PolyU(4)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
GLN, Linear-3, PolyU(4)
Plasmid#166985PurposeTests for the impact of 4 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.DepositorInsertTermination module:PolyU(4)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A,C4A,C5A,C7A-RFP
Plasmid#166842PurposeExpresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsRFPExpressionYeastMutationG2A, C4A, C5A, C7APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A,C4A,C5A,C7A-GFP
Plasmid#166839PurposeExpresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationG2A, C4A, C5A, C7APromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEL2082
Plasmid#155162PurposeExpresses (Strep-MlaF_Δ[248-269])-(6xHis-TEV-MlaB)DepositorInsert(MlaFΔ[248-269])-(6xHis-TEV-MlaB)
UseTagsExpressionBacterialMutationdeleted amino acids 248-269 in MlaFPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1514
Plasmid#155147PurposeExpresses MlaFEDCB operon with MlaF C-terminus deleted [1-246], His-tag on MlaDDepositorInsertmlaF(1-246)-mlaE-[6xHis-2xQH-TEV-mlaD]-mlaCB operon
UseTagsExpressionMutationPromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1965
Plasmid#155152PurposeExpresses MlaF(1-246)-(6 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
UseTagsExpressionMutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1966
Plasmid#155153PurposeExpresses MlaF(1-246)-(10 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
UseTagsExpressionMutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEL1967
Plasmid#155154PurposeExpresses MlaF(1-246)-(14 aa linker)-GCN4DepositorInsertmlaF(1-246)-GCN4_Dimerization_Domain
UseTagsExpressionMutationdeleted amino acids 247-269 in MlaF; added dimeri…PromoteraraBADAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1_PolyU(1)
Plasmid#159479PurposeTests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(1)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1_PolyU(2)
Plasmid#159480PurposeTests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(2)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Linear-1_PolyU(3)
Plasmid#159481PurposeTests for the impact of 3 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.DepositorInsertTermination Module:Linear-1_PolyU(3)
UseTagsExpressionMammalianMutationPromoterU6-27Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
OA-1052B (Ubiq-Fluc_Ubiq-Rluc)
Plasmid#132426PurposeExpress Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoterDepositorInsertsUbiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-ydfHp-GFPuv
Plasmid#139079PurposeLow phosphate inducible promoter ydfHp for GFP expressionDepositorInsertydfHp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phnCp-GFPuv
Plasmid#139080PurposeLow phosphate inducible promoter phnCp for GFP expressionDepositorInsertphnCp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-tktBp-GFPuv
Plasmid#139081PurposeLow phosphate inducible promoter tktBp for GFP expressionDepositorInserttktBp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-asrp-GFPuv
Plasmid#139082PurposeLow phosphate inducible promoter asrp for GFP expressionDepositorInsertasrp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yegHp-GFPuv
Plasmid#139084PurposeLow phosphate inducible promoter yegHp for GFP expressionDepositorInsertyegHp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-ytfKp-GFPuv
Plasmid#139085PurposeLow phosphate inducible promoter ytfKp for GFP expressionDepositorInsertytfKp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-amnp-GFPuv
Plasmid#139070PurposeLow phosphate inducible promoter amnp for GFP expressionDepositorInsertamnp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-mipAp-GFPuv
Plasmid#139071PurposeLow phosphate inducible promoter mipAp for GFP expressionDepositorInsertmipAp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoEp-GFPuv
Plasmid#139074PurposeLow phosphate inducible promoter phoEp for GFP expressionDepositorInsertphoEp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoHp1-GFPuv
Plasmid#139075PurposeLow phosphate inducible promoter phoHp1 for GFP expressionDepositorInsertphoHp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-phoUp-GFPuv
Plasmid#139076PurposeLow phosphate inducible promoter phoUp for GFP expressionDepositorInsertphoUp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-pstSp-GFPuv
Plasmid#139077PurposeLow phosphate inducible promoter pstSp for GFP expressionDepositorInsertpstSp-GFPuv
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB HTB_hMSH6_T1219D
Plasmid#135466PurposeExpression human MSH6_T1219D mutant protein using baculovirusDepositorInserthuman MSH6_T1219D (MSH6 Human)
UseTagsHis-tagExpressionInsectMutationchanged threonine to aspartic acidPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNIC28-BSA4-S15
Plasmid#127821PurposeProduction of recombinant human ribosomal protein S15DepositorInsertRPS15 (RPS15 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNH-TrxT-G-S10
Plasmid#127829PurposeProduction of recombinant human ribosomal protein S10DepositorInsertRPS10 (RPS10 Human)
UseTagsHis6 - Thioredoxin - TEVExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-4xUAS
Plasmid#125149PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in fly cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI126
Plasmid#126066PurposeModule 1 vector of PlaMiNGo toolkit for expression of plastid targeted GFP1-10 in plant cellDepositorInsertChloroplastic FNR transit peptide
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoter'long' CaMV35SAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI127
Plasmid#126067PurposeModule 1 vector of PlaMiNGo toolkit for expression of mitochondria targeted GFP1-10 in plant cellDepositorInsertmitochondria Rieske iron sulphur protein (1-100 aa)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoter'long' CaMV35SAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI177
Plasmid#126077PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP1-10
UseBinary, module 1Tagslinker (GTGGGGS)ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI129
Plasmid#126210PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI161
Plasmid#126211PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11
UseBinary, module 1TagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_W22HDHFR_WTTS
Plasmid#91241Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationW22HPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_F31VDHFR_WTTS
Plasmid#91227Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationF31VPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only