We narrowed to 9,339 results for: UTY

1. APEX2 - Actin in pEGFP

   Plasmid

   #66172

   Purpose

   APEX2 fused to the N-terminus of human beta actin

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 - human beta Actin (ACTB Human, Synthetic)
   

   Use

   Tags

   Flag epitope tag (DYKDDDDK)

   Expression

   Mammalian

   Mutation

   APEX2 contains 4 mutations relative to wt soybean…

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nsp6-mCherry

   Plasmid

   #165133

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp6 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-HA-Flag-natT1R2 ECD-MHC

   Plasmid

   #113957

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG, Signal/leader sequence from influenza hemag…

   Expression

   Mammalian

   Mutation

   residues 22-568 fused to a transmembrane tether

   Promoter

   cmv

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sec61β-Flag-APEX2-SOPP3

   Plasmid

   #229072

   Purpose

   Expresse APEX2-SOPP3 in ER in mammalian cell

   Depositor

   Weijun Pan

   Article

   Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

   Insert

   Express APEX2-SOPP3 in the ER (APEX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcBac1-BacMam-4x-PyOtR

   Plasmid

   #217364

   Purpose

   Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   4 copies of pyrrolysyl-tRNA mutant PyOtR
   

   Use

   Baculoviral

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Nsp13-mCherry

   Plasmid

   #165136

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp13 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV-hSyn-DIO-mCReP

   Plasmid

   #167503

   Purpose

   Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

   Depositor

   Nicole Calakos

   Article

   Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

   Insert

   Ppp1r15b (Ppp1r15b Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-ITR-PytR(A2.1)-mCherry

   Plasmid

   #204869

   Purpose

   Expresses pyrrolysyl tRNA variant "A2.1" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   M. mazei pyrrolysyl tRNA mutant "A2.1"
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4G, T65C, T66C, T67T

   Promoter

   U6

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5-TEX264-deltaLRR-cSSH

   Plasmid

   #220210

   Purpose

   Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

   Depositor

   Kristijan Ramadan

   Article

   Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

   Insert

   TEX264 (TEX264 Human)
   

   Use

   Tags

   Strep-strep-HA

   Expression

   Mammalian

   Mutation

   c.4-75del / p.2-24del

   Promoter

   CMV/TetO

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTP434

    Plasmid

    #104167

    Purpose

    Bacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labeling

    Depositor

    Dirk Görlich

    Article

    Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

    Insert

    Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
    

    Use

    Tags

    14x Histidine tag and NEDD8 from Brachypodium dis…

    Expression

    Bacterial

    Mutation

    3x Cysteines located at bps 493-495, 520-522, and…

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-shLuc.mKO2

    Plasmid

    #85224

    Purpose

    shLuc (Target TTACGCTGAGTACTTCGA) for silencing luciferase gene as a control and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. H2B-GFP-Aurora B WT

    Plasmid

    #184042

    Purpose

    Expresses H2B-GFP fused to Aurora B WT

    Depositor

    Lienhard Schmitz

    Article

    Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

    Insert

    AURKB (AURKB Human)
    

    Use

    Tags

    EGFP and H2B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330-COSMC-KO

    Plasmid

    #80008

    Purpose

    gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    COSMC (C1GALT1C1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-FLAG-Tev_ACTA1 (alpha-actin)

    Plasmid

    #188452

    Purpose

    Express in mammalian cells human alpha-actin (ACTA1) with N-terminal 6xHis tag, FLAG tag, and TEV protease site.

    Depositor

    Roberto Dominguez

    Article

    Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

    Insert

    ACTA1 (ACTA1 Human)
    

    Use

    Tags

    6xHis-FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDYT002

    Plasmid

    #186550

    Purpose

    Lineage tracing vector (with barcoded Target Sites and triple sgRNAs

    Depositor

    Jonathan Weissman

    Article

    Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

    Insert

    14-bp random integration barcode and three target sites
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Nsp7-EGFP

    Plasmid

    #165111

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2

    Plasmid

    #183352

    Purpose

    Flp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    TVA, EGFP and tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEMS2113

    Plasmid

    #49138

    Purpose

    AAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    ssAAV-Ple67-emGFP-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FEV

    Available since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAAV-ITR-EcYtR(NGS-6)-mCherry

    Plasmid

    #217363

    Purpose

    Expresses E. coli tyrosine tRNA variant "NGS-6" and a wild-type mCherry reporter; can be packaged into AAV

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

    Insert

    E. coli tyrosine tRNA mutant, "NGS-6"
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMEXneo-TrkB

    Plasmid

    #190203

    Purpose

    To express the Human TrkB receptor under the Moloney mouse sarcoma virus LTR

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

    Insert

    TrkB (NTRK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney mouse sarcoma virus right LTR

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOGS539_GLuc-ns

    Plasmid

    #226719

    Purpose

    Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

    Depositor

    Gautam Dantas

    Article

    Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

    Insert

    Gaussia Luciferase
    

    Use

    Tags

    HA tag

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PL-5LTR-GW-A

    Plasmid

    #138480

    Purpose

    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSEMS-Tom20-mEGFP-reHaloTagS

    Plasmid

    #200189

    Purpose

    Tom20 with reversible HaloTagS variant for fluorescent labeling

    Depositor

    Jacob Piehler

    Article

    Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

    Insert

    Tom20 (TOMM20 Human)
    

    Use

    Tags

    mEGFP and reHaloTagS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-A3A(N57Q)-BE3

    Plasmid

    #158157

    Purpose

    T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

    Depositor

    Keith Joung

    Article

    Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

    Insert

    6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
    

    Use

    Tags

    6xHis tag on N-terminus

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTarget: Ptaq-RFP_PlacIq-GFP

    Plasmid

    #192280

    Purpose

    RFP expressed under constitutive promoter Ptaq and GFP expressed under constitutive promoter Placiq

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Inserts

    RFP
    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacIq and Ptaq

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMH-SFB-USP7

    Plasmid

    #99393

    Purpose

    Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged USP7

    Depositor

    Michael Huen

    Article

    An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    USP7 (USP7 Human)
    

    Use

    Tags

    SFB (S protein-FLAG-Streptavidin binding peptide)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEMS1991

    Plasmid

    #49122

    Purpose

    AAV plasmid with FEV (Ple67) promoter driving expression of iCre. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    ssAAV-Ple67-iCre-WPRE
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    FEV

    Available since

    May 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pGBT9-Hook3

    Plasmid

    #198525

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook3 (HOOK3 Human)
    

    Use

    Tags

    GAL4-DNA binding domain (BD) fragment

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTetON-BMP2/7

    Plasmid

    #137913

    Purpose

    Inducible mammalian co-expression vector for human bone morphogenetic protein 2 and 7 cDNAs (2 transcriptional units, divergent)

    Depositor

    Georg Feichtinger

    Article

    Feichtinger et al Eur Cell Mater. 2014 Feb 19;27:166-84; d

    Inserts

    BMP2 (BMP2 Human)
    BMP7 (BMP7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKLV2-U6gRNA5(Pou5f1-g2)-PGKpuroBFP-W

    Plasmid

    #105036

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pou5f1 (Pou5f1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAdCMV/V5-DEST-STAT3C-3xFlag

    Plasmid

    #99264

    Purpose

    Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

    Depositor

    Alejandro Adam

    Article

    Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

    Insert

    Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)
    

    Use

    Adenoviral

    Tags

    3x-Flag

    Expression

    Mutation

    A662C N664C

    Promoter

    CMV

    Available since

    Jan. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Human USP7-3X FLAG

    Plasmid

    #225334

    Purpose

    Lentiviral expression of human USP7 with 3x FLAG tag in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    USP7 (USP7 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHRIG-PRAS40

    Plasmid

    #53599

    Purpose

    lentiviral expression of constitutively active PRAS40 and EGFP

    Depositor

    Heng Zhao

    Article

    Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1

    Insert

    PRAS40 (AKT1S1 Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP and myc

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pENTR-AmCyan

    Plasmid

    #138481

    Purpose

    For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    AmCyan
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AAV pCAG-mRuby3-WPRE

    Plasmid

    #107744

    Purpose

    Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

    Depositor

    Rylan Larsen

    Article

    Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

    Insert

    mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLV[Exp]-Puro-EF-1α>{H2B-RFP}

    Plasmid

    #201121

    Purpose

    RFP expression in cells under EF-1α promoter; cytoplasmic and histone-bound, nuclear

    Depositor

    Ori Bar-Nur

    Article

    Zvick et al Stem Cell Reports. 2022 Sep 13;17(9):194

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    No

    Promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Nsp14-mCherry

    Plasmid

    #165137

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp14 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. human ETV6 gRNA-1

    Plasmid

    #133353

    Purpose

    human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

    Depositor

    Linzhao Cheng

    Article

    Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

    Insert

    ETV6 (ETV6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLINK cyclin T1 (1-280) myc (K93D) (P#580)

    Plasmid

    #14611

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) K93D (CCNT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K93D. Deficient in binding to CDK9. Inactive in 3…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pLPC-Progerin-CS

    Plasmid

    #69064

    Purpose

    encodes Progerin with a substitution of cysteine in CaaX-motif by a serine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

    Insert

    Progerin (LMNA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Progerin with substitution of cysteine in CaaX-mo…

    Promoter

    CMV

    Available since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

41. pX330-MGAT1-KO

    Plasmid

    #80009

    Purpose

    gRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.

    Depositor

    Sriram Neelamegham

    Article

    Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

    Insert

    MGAT1 (MGAT1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pET28-HADH2

    Plasmid

    #67863

    Purpose

    bacterial expression of SDR5C1 (HSD17B10), full coding sequence + N-term. His tag (thrombin site)

    Depositor

    Walter Rossmanith

    Article

    Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

    Insert

    HADH2 (HSD17B10 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJM186A

    Plasmid

    #218127

    Purpose

    Hybrid circuit displaying hsCRBN bait and driving expression of gIII

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Inserts

    CRBN (CRBN Human)
    M13 pIII
    

    Use

    Tags

    434cI_RR69

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pRRL-SFFV-rtTA3-IRES-Luc-P2A-mtagBFP

    Plasmid

    #176027

    Purpose

    construct expresses the reverse tet-responsive transactivator 3 (rtTA3) together with Luciferase (Luc) for bioluminescence in vivo imaging, and mTaqBFP for enriching transgenic cells by flow cytometry

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    rtTA3
    Luc2
    mtagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Nsp13 K289A-EGFP

    Plasmid

    #165130

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp13 K289A (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. EGFP-Nsp8

    Plasmid

    #165114

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp8 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMEXneo-TrkC

    Plasmid

    #190202

    Purpose

    To express the Human TrkC receptor under the Moloney mouse sarcoma virus LTR.

    Depositor

    Pantelis Tsoulfas

    Article

    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.

    Insert

    TrkC (NTRK3 Human)
    

    Use

    Eucaryotic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney mouse sarcoma virus LTR

    Available since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pC1-IMS-oROS-HT

    Plasmid

    #216419

    Purpose

    Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. FusionRed-Fibrillarin-7

    Plasmid

    #56118

    Purpose

    Localization: Nucleoli, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    R36Q, S66F

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

50. pCSCRET2(#323)

    Plasmid

    #184058

    Purpose

    cyclofen-inducible CRE activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

    Insert

    6myc-CRE-ERT2
    

    Use

    Synthetic Biology

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge