We narrowed to 13,583 results for: TIM

1. pNB753

   Plasmid

   #60148

   Purpose

   Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   GrLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   LEU1pr

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. EKAR2G_design2_mTFP_105_Venus_157

   Plasmid

   #40000

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

3. EKAR2G_design1_mTFP_175_Venus_wt

   Plasmid

   #39828

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

4. EKAR2G_design1_mTFP_227_Venus_wt

   Plasmid

   #39833

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

5. EKAR2G_design1_mTFP_159_Venus_wt

   Plasmid

   #39823

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

6. XE95 mFZ6-CS2+

   Plasmid

   #16802

   Depositor

   Randall Moon

   Article

   Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

   Insert

   mFZ6-CS2+ (Fzd6 Mouse)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pRSET FLIPE intramol-10u

   Plasmid

   #13550

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

   Insert

   FLIP-E intramol A207R (gltI E. coli)
   

   Use

   Tags

   EYFP

   Expression

   Bacterial

   Mutation

   A207R, ECFP insert in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. XE105 XPKC alpha-myc-CS2+

   Plasmid

   #16745

   Depositor

   Randall Moon

   Article

   Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

   Insert

   XPKC-alpha-myc (prkca Frog)
   

   Use

   Xenopus expression

   Tags

   c-myc

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-CAG-Grx1-RoTq-On

   Plasmid

   #234467

   Purpose

   Mammalian expression of Grx1-RoTq-On

   Depositor

   Gary Yellen

   Article

   Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

   Insert

   Grx1-RoTq-On
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pG0A0-1-1-PBAD-dCas9

    Plasmid

    #208815

    Purpose

    Expresses arabinose-inducible dCas9, codon-optimized for Salmonella

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    Arabinose-inducible dCas9 that is codon optimized for expression in Salmonella
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinsoe-inducible promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-His-E1GFP

    Plasmid

    #233885

    Purpose

    Bacterial Expression of recombinant His-tagged E1GFP

    Depositor

    Francesco Cardarelli

    Article

    De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

    Insert

    E1GFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    T65S and T203Y compared to EGFP

    Promoter

    T7 promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS413-TEF1pr-CFAPorig

    Plasmid

    #221092

    Purpose

    FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS413-TEF1pr-NFAPorig

    Plasmid

    #221091

    Purpose

    FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS415-TEF1pr-NFAPorig

    Plasmid

    #221086

    Purpose

    FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS415-TEF1pr-CFAPorig

    Plasmid

    #221087

    Purpose

    FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDIV302

    Plasmid

    #204973

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and nat1 resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    nat1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid

    #227645

    Purpose

    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

    Depositor

    Richard Murray

    Article

    Kapasiawala et al bioRxiv 2023.03.22.533877

    Insert

    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUB_SM-RLuc0---MS2

    Plasmid

    #210572

    Purpose

    Expresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpins

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-nonoptimal Renilla luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    UBC human

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUB_SM-RLuc100---MS2

    Plasmid

    #210573

    Purpose

    Expresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpins

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-optimal Renilla luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    UBC human

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1_SM-FLuc0-malat1

    Plasmid

    #210580

    Purpose

    Expresses SM(FLAG)-nonoptimal Firefly - 3'UTR malat1 triple helix

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-nonoptimal FLuc - 3'UTR malat1 triple helix
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1_SM-FLuc100-malat1

    Plasmid

    #210581

    Purpose

    Expresses SM(FLAG)-optimal Firefly luciferase - 3'UTR malat1 triple helix

    Depositor

    Olivia Rissland

    Article

    Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

    Insert

    Spaghetti monster (FLAG)-optimal FLuc - 3'UTR malat1 triple helix
    

    Use

    Luciferase

    Tags

    Spaghetti monster (FLAG)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUCmini-UGCG-EGFP

    Plasmid

    #225878

    Purpose

    Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

    Depositor

    Yohei Sato

    Article

    Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRSETA-qmTQ2-ATP

    Plasmid

    #225247

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imaging

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-ATP
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRSETA-qmTQ2-glucose

    Plasmid

    #225252

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucose

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-glucose
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSETA-qmTQ2-cAMP

    Plasmid

    #225249

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. A99-ELP

    Plasmid

    #170760

    Purpose

    A99 laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    A99-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AG73-ELP

    Plasmid

    #170762

    Purpose

    AG73 laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    AG73-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EF1m-ELP

    Plasmid

    #170763

    Purpose

    EF1m laminin α1 peptide fused to I30-ELP (VPGIG)30

    Depositor

    Andrew MacKay

    Article

    Truong et al Biomacromolecules. 2024 Jul 8;25(7):4001

    Insert

    EF1m-ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSpyA-GFP

    Plasmid

    #220189

    Purpose

    sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pEM7 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pEM7

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSpyB-GFP

    Plasmid

    #220190

    Purpose

    sgRNA expression vector - pBG35 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG35

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge