19,181 results

1. pAAV-Ef1a-sRGECO

   Plasmid

   #137125

   Purpose

   Viral expression of sRGECO

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   sRGECO
   

   Use

   AAV

   Tags

   Expression

   Mutation

   E217D

   Promoter

   EF1a

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Dronpa3-N1

   Plasmid

   #54682

   Purpose

   Localization: N1 Cloning Vector, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   Dronpa3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pcDNA3/RGS-UBC12

   Plasmid

   #20080

   Depositor

   Edward Yeh

   Article

   Gong et al J Biol Chem. 1999 Apr 23;274(17):12036-4

   Insert

   UBC12 (UBE2M Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pMOS023: Peredox NADH/NAD+ sensor (cytosolic)

   Plasmid

   #163060

   Purpose

   Peredox NADH/NAD+ sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Peredox
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Creb1

   Plasmid

   #154942

   Purpose

   Expresses Creb1 in mammalian cells

   Depositor

   Georgios Stathopoulos

   Insert

   Creb1 (Creb1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti CMV/TO GFP-MDC1 (779-2)

   Plasmid

   #26285

   Depositor

   Eric Campeau

   Insert

   Mediator of DNA damage checkpoint 1 (MDC1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p5154

   Plasmid

   #175390

   Purpose

   High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)

   Depositor

   Michael Wiles

   Article

   Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting; Cloning vector, to be used to de…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSGNluc

   Plasmid

   #186705

   Purpose

   A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

   Depositor

   Maria Leptin, Baubak Bajoghli

   Article

   Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

   Inserts

   GFP
   luciferase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-p62

   Plasmid

   #28027

   Purpose

   Mammalian expression of HA tagged human p62

   Depositor

   Qing Zhong

   Article

   Fan et al Autophagy. 2010 Jul 22. 6(5):.

   Insert

   p62 (SQSTM1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-cdc20

    Plasmid

    #11594

    Depositor

    Marc Kirschner

    Article

    Pfleger et al Genes Dev. 2001 Sep 15. 15(18):2396-407.

    Insert

    cdc20 (CDC20 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMOS008: GCaMP6F calcium sensor (cytosolic)

    Plasmid

    #163045

    Purpose

    GCaMP6F calcium sensor (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    GCaMP6F
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CMV-LAMP1-HaloTag

    Plasmid

    #164209

    Purpose

    Expresses HaloTag tagged LAMP1 under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mPiezo2-CMV-Sport6

    Plasmid

    #81073

    Purpose

    mouse Piezo2

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Nat Commun. 2015 May 26;6:7223. doi: 10.

    Insert

    Piezo2 (please see depositor comments below) (Piezo2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TIGRE docking site targeting vector

    Plasmid

    #61580

    Purpose

    Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti6.3/EV

    Plasmid

    #120848

    Purpose

    Control vector, 20nt random sequence between gateway recombination sites

    Depositor

    Jan Rehwinkel

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pgRNA-humanized

    Plasmid

    #44248

    Purpose

    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1/Myc-His(-)-HSulf-2

    Plasmid

    #13004

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 (SULF2 Human)
    

    Use

    Tags

    6x His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOpen-MMLV_RT (lack H)

    Plasmid

    #165556

    Purpose

    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. GPD-roGFP

    Plasmid

    #49436

    Purpose

    Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

    Depositor

    Paul Schumacker

    Article

    Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

    Insert

    GPD-roGFP2
    

    Use

    Adenoviral

    Tags

    Glycerol phosphate dehydrogenase, 5' to roGF…

    Expression

    Mammalian

    Mutation

    C48S, Q80R, S147C, and Q204C in Clontech's G…

    Promoter

    CMV

    Available since

    Dec. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SunTagng22aa

    Plasmid

    #106438

    Purpose

    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGWB517

    Plasmid

    #74859

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Gamillus/ pcDNA3

    Plasmid

    #124837

    Purpose

    mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

    Depositor

    Takeharu Nagai

    Article

    Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

    Insert

    Gamillus
    

    Use

    Tags

    No tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. FTO-dCas9

    Plasmid

    #134781

    Purpose

    Expression fusion protein FTO-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    FTO and inactive dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. bZiPro

    Plasmid

    #86846

    Purpose

    express NS2B-NS3 protease in E.coli

    Depositor

    Dahai Luo

    Article

    Zhang et al Science. 2016 Dec 8. pii: aai9309.

    Inserts

    NS3 1-177
    NS2B45-96
    

    Use

    Tags

    a hexahistidine tag and a thrombin cleavage site

    Expression

    Bacterial

    Mutation

    NS2B cofactor region (residues 45–96) and NS3 pro…

    Promoter

    T7

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pREDawn-AmpR-MCS

    Plasmid

    #188978

    Purpose

    For red light-induced bacterial expression of gene of interest (AmpR)

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMSCV-Blasticidin

    Plasmid

    #75085

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-N1-TFE3

    Plasmid

    #38120

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFE3 (TFE3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDTA-TK

    Plasmid

    #22677

    Depositor

    Robert Benezra

    Article

    Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

    Insert

    DTA and TK
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    pDTA-TK dual selection plasmid for targeting vect…

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Pet28a_Synthetic_Human_H2A.1

    Plasmid

    #42634

    Depositor

    Joe Landry

    Insert

    Human H2A.1 (H2AC13 Synthetic, Human)
    

    Use

    Tags

    Untagged

    Expression

    Bacterial

    Mutation

    Codon Optimized

    Promoter

    T7

    Available since

    Feb. 25, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. mCherry-Smoothened1-N-18

    Plasmid

    #55134

    Purpose

    Localization: Cilium, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Smoothened1
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge