We narrowed to 7,009 results for: mme

1. pETDuet1-LanCL2-H264A-C-Ter-His

   Plasmid

   #154187

   Purpose

   To express LanCL2-H264A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His264->Ala264

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-15b-LanCL1-H219A

   Plasmid

   #154190

   Purpose

   To express LanCL1-H219A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His219->Ala219

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-15b-LanCL1-C322A

   Plasmid

   #154191

   Purpose

   To express LanCL1-C322A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Cys322->Ala322

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMAL-p2x-LanCL2

   Plasmid

   #154192

   Purpose

   To express MBP-LanCL2 in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Maltose binding protein (MBP)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pX458-IAP-del-gRNA1

   Plasmid

   #185002

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   IAP-del-gRNA1 (Akp3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX458-IAP-del-gRNA2

   Plasmid

   #185003

   Purpose

   expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   IAP-del-gRNA2 (Akp3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KTK_095

   Plasmid

   #180524

   Purpose

   dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KTK_324

   Plasmid

   #180566

   Purpose

   Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFUW-TetO-NMI

   Plasmid

   #178446

   Purpose

   doxycycline-inducible expression of Human NMI in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   NMI (NMI Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFUW-TetO-BATF2

    Plasmid

    #178453

    Purpose

    doxycycline-inducible expression of Human BATF2 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

    Insert

    BATF2 (BATF2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSIN-Gapdh

    Plasmid

    #175938

    Purpose

    knockout mouse Gapdh

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Gapdh gRNA (Gapdh Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SIN40C.SFFV.mTagBFP2.miR-125b-2

    Plasmid

    #169311

    Purpose

    Constitutive overexpression of miR-125b-2 with mTagBFP2 as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    miR-125b-2 (MIR125B2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS313_ATP3G919C

    Plasmid

    #120255

    Purpose

    Expression of ATP3-7 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    G919 changed to C; Alanine 307 changed to Proline

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS313_ATP3mut

    Plasmid

    #120256

    Purpose

    Expression of ATP3-67 in yeast

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

    Insert

    ATP3 (ATP3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 304 changed to Asparagine; Alanine 307…

    Promoter

    ATP3

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBB0238-TEV-His12

    Plasmid

    #137038

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0238 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66635.2
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV-IRES-GFP-MD2 (F126A)-HA

    Plasmid

    #133067

    Purpose

    Retroviral expression of mouse MD2 (F126A) tagged with HA

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    MD2 (F126A) (Ly96 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    F126A

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMSCV-IRES-GFP-MD2 (C95A)-HA

    Plasmid

    #133066

    Purpose

    Retroviral expression of mouse MD2 (C95A) tagged with HA

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    MD2 (C95A) (Ly96 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    C95A

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSR357.12

    Plasmid

    #125107

    Purpose

    Expression of narL(1-134aa)-ydfI(129-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)134
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    A84T - see depositor comments below and Q183K-see…

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDDGFP-Leu2d-GgKDELR2 R159K

    Plasmid

    #123626

    Purpose

    Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg159Lys

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDDGFP-Leu2d-GgKDELR2 H12A

    Plasmid

    #123619

    Purpose

    Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    His12Ala

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDDGFP-Leu2d-GgKDELR2 R47A

    Plasmid

    #123620

    Purpose

    Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg47Ala

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDDGFP-Leu2d-GgKDELR2 R47K

    Plasmid

    #123621

    Purpose

    Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg47Lys

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDDGFP-Leu2d-GgKDELR2 E127A

    Plasmid

    #123622

    Purpose

    Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Glu127Ala

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDDGFP-Leu2d-GgKDELR2 E127Q

    Plasmid

    #123623

    Purpose

    Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Glu127Gln

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDDGFP-Leu2d-GgKDELR2 Y158F

    Plasmid

    #123624

    Purpose

    Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Tyr158Phe

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDDGFP-Leu2d-GgKDELR2 R159A

    Plasmid

    #123625

    Purpose

    Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg159Ala

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SMR3B-bio-His

    Plasmid

    #52183

    Purpose

    Expresses full-length Submaxillary gland androgen-regulated protein 3B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SMR3B (SMR3B Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTYB-pLAC-I68S/I73S

    Plasmid

    #48728

    Purpose

    Bacterial expression of Lacritin Proteins with Mutation at protein 68 and 73 from Isoleucine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Isoleucine 68 and 73 to Serine

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pTYB-pLAC-L108S

    Plasmid

    #48725

    Purpose

    Bacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to Serine

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Leucine 108 to Serine

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pTYB1-pLAC-N71

    Plasmid

    #48720

    Purpose

    Bacterial expression of Lacritin protein with 70 AA removed from the N-terminus

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Deleted first 71 amino acids of mature lacritin w…

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pTYB1-pLAC-N45

    Plasmid

    #48718

    Purpose

    Bacterial expression of Lacritin protein with 45 AA removed from N terminus

    Depositor

    Gordon Laurie

    Article

    Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

    Insert

    Lacritin (LACRT Human)
    

    Use

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Deleted first 45 amino acids of mature lacritin w…

    Promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

32. pBp-FGFR2c-E471Q

    Plasmid

    #45704

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    E471Q

    Promoter

    Available since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pEGFP-N1/PD-L1

    Plasmid

    #121478

    Purpose

    expression of human PD-L1 in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. paGFP-Omp25

    Plasmid

    #69598

    Purpose

    expresses GFP-labeled mitochondrial outer membrane protein 25

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    paGFP-Omp25 (Synj2bp Rat)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    photoactivatable GFP fused to rat Omp25 C-terminus

    Promoter

    CMV

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ICAM5-bio-His

    Plasmid

    #51759

    Purpose

    Expresses full-length Intercellular adhesion molecule 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ICAM5 (ICAM5 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-hSyn-FLEX-TeLC-P2A-dTomato

    Plasmid

    #159102

    Purpose

    Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

    Depositor

    Sandeep Datta

    Article

    Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

    Insert

    TeLC-P2A-NLS-dTomato
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLVX-Flag-MIB1-IRES-Puro

    Plasmid

    #140240

    Purpose

    Lentiviral vector expressing flag-tagged MIB1

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Insert

    Flag-MIB1 (MIB1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CRISPR-SB

    Plasmid

    #177936

    Purpose

    Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

    Depositor

    Roland Rad

    Article

    Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

    Type

    Empty backbone

    Use

    CRISPR; Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pT3-myr-AKT-HA

    Plasmid

    #31789

    Depositor

    Xin Chen

    Article

    Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3-HA-BHLHE40

    Plasmid

    #110154

    Purpose

    Expression of human BHLHE40 in mammalian cells

    Depositor

    Daniele Guardavaccaro

    Article

    Kim et al Mol Cell Biol. 2014 Nov 15;34(22):4177-8

    Insert

    BHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge